神經(jīng)膠質(zhì)纖維酸性蛋白(GFAP)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)
ELISA Kit for Glial Fibrillary Acidic Protein (GFAP)
Intermediate Filament Protein
- 編號SEA068Mu
- 物種Mus musculus (Mouse，小鼠) 相同的名稱，不同的物種。
- 實(shí)驗(yàn)方法雙抗夾心
- 反應(yīng)時(shí)長3h
- 檢測范圍15.6-1,000pg/mL
- 靈敏度最小可檢測劑量小于等于6.0pg/mL.
- 樣本類型serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下載 英文說明書 中文說明書
- 規(guī)格 48T96T 96T*5 96T*10 96T*100
- 價(jià)格 ¥ 2722 ¥ 3888 ¥ 17496 ¥ 33048 ¥ 272160
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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實(shí)驗(yàn)結(jié)果圖
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標(biāo)準(zhǔn)曲線圖
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通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
本試劑盒用于檢測神經(jīng)膠質(zhì)纖維酸性蛋白(GFAP)，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來源的限制，不可能完成對所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應(yīng)。
回收率
分別于定值血清及血漿樣本中加入一定量的神經(jīng)膠質(zhì)纖維酸性蛋白(GFAP)（加標(biāo)樣品），重復(fù)測定并計(jì)算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 80-102 | 83 |
| EDTA plasma(n=5) | 87-96 | 92 |
| heparin plasma(n=5) | 85-98 | 88 |
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進(jìn)行定量檢測,每份樣本連續(xù)測定20 次，分別計(jì)算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進(jìn)行定量測定，每個樣本使用同一試劑盒重復(fù)測定8次，分別計(jì)算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的神經(jīng)膠質(zhì)纖維酸性蛋白(GFAP)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中神經(jīng)膠質(zhì)纖維酸性蛋白(GFAP)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 88-101% | 91-98% | 78-101% | 78-92% |
| EDTA plasma(n=5) | 88-101% | 94-102% | 78-94% | 92-105% |
| heparin plasma(n=5) | 99-105% | 88-105% | 96-105% | 96-104% |
穩(wěn)定性
經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實(shí)驗(yàn)室的環(huán)境條件需盡量保持一致，尤其是實(shí)驗(yàn)室內(nèi)溫度、濕度及溫育條件。其次由同一實(shí)驗(yàn)員來進(jìn)行操作可減少人為誤差。
實(shí)驗(yàn)流程
1. 實(shí)驗(yàn)前標(biāo)準(zhǔn)品、試劑及樣本的準(zhǔn)備；
2. 加樣（標(biāo)準(zhǔn)品及樣本）100µL，37°C孵育1小時(shí)；
3. 吸棄，加檢測溶液A100µL，37°C孵育1小時(shí)；
4. 洗板3次；
5. 加檢測溶液B100µL，37°C孵育30分鐘；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分鐘；
8. 加終止液50µL，立即450nm讀數(shù)。
實(shí)驗(yàn)原理
將神經(jīng)膠質(zhì)纖維酸性蛋白(GFAP)抗體包被于96孔微孔板中，制成固相載體，向微孔中分別加入標(biāo)準(zhǔn)品或標(biāo)本，其中的神經(jīng)膠質(zhì)纖維酸性蛋白(GFAP)與連接于固相載體上的抗體結(jié)合，然后加入生物素化的神經(jīng)膠質(zhì)纖維酸性蛋白(GFAP)抗體，將未結(jié)合的生物素化抗體洗凈后，加入HRP標(biāo)記的親和素，再次徹底洗滌后加入TMB底物顯色。TMB在過氧化物酶的催化下轉(zhuǎn)化成藍(lán)色，并在酸的作用下轉(zhuǎn)化成最終的黃色。顏色的深淺和樣品中的神經(jīng)膠質(zhì)纖維酸性蛋白(GFAP)呈正相關(guān)。用酶標(biāo)儀在450nm波長下測定吸光度(O.D.值)，計(jì)算樣品濃度。
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Mus musculus (Mouse，小鼠) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| RPA068Mu03 | 神經(jīng)膠質(zhì)纖維酸性蛋白(GFAP)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA068Mu02 | 神經(jīng)膠質(zhì)纖維酸性蛋白(GFAP)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA068Mu01 | 神經(jīng)膠質(zhì)纖維酸性蛋白(GFAP)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA068Mu04 | 神經(jīng)膠質(zhì)纖維酸性蛋白(GFAP)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA068Mu03 | 神經(jīng)膠質(zhì)纖維酸性蛋白(GFAP)多克隆抗體 | WB; IHC; ICC; IP. |
| PAA068Mu02 | 神經(jīng)膠質(zhì)纖維酸性蛋白(GFAP)多克隆抗體 | WB; IHC; ICC; IP. |
| PAA068Mu01 | 神經(jīng)膠質(zhì)纖維酸性蛋白(GFAP)多克隆抗體 | WB; IHC; ICC; IP. |
| PAA068Mu04 | 神經(jīng)膠質(zhì)纖維酸性蛋白(GFAP)多克隆抗體 | WB; IHC; ICC; IP. |
| MAA068Mu22 | 神經(jīng)膠質(zhì)纖維酸性蛋白(GFAP)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA068Mu23 | 神經(jīng)膠質(zhì)纖維酸性蛋白(GFAP)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA068Mu21 | 神經(jīng)膠質(zhì)纖維酸性蛋白(GFAP)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA068Mu24 | 神經(jīng)膠質(zhì)纖維酸性蛋白(GFAP)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA068Mu25 | 神經(jīng)膠質(zhì)纖維酸性蛋白(GFAP)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA068Mu27 | 神經(jīng)膠質(zhì)纖維酸性蛋白(GFAP)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA068Mu28 | 神經(jīng)膠質(zhì)纖維酸性蛋白(GFAP)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA068Mu29 | 神經(jīng)膠質(zhì)纖維酸性蛋白(GFAP)單克隆抗體 | WB; IHC; ICC; IP. |
| SEA068Mu | 神經(jīng)膠質(zhì)纖維酸性蛋白(GFAP)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| MEA068Mu | 神經(jīng)膠質(zhì)纖維酸性蛋白(GFAP)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,小樣本) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA068Mu | 神經(jīng)膠質(zhì)纖維酸性蛋白(GFAP)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| KSA068Mu01 | 神經(jīng)膠質(zhì)纖維酸性蛋白(GFAP)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| Journal of Occupational Health | Effects of Exposure to 1-Bromopropane on Astrocytes and Oligodendrocytes in Rat Brain [Pubmed: 23183024] |
| Morfovirtual 2014 | EFECTO PROTECTOR DEL TRATAMIENTO CON NEUROEPO SOBRE LA GLIA DE LA CORTEZA CEREBRAL EN RATONES TRANSGéNICOS CON LA ENFERMEDAD DE ALZHEIMER. [Sld:Source] |
| Annals of Anatomy | Effect of memantine, a NMDA receptor blocker, on ethambutol-induced retinal injury [PubMed: 26704355] |
| Mediators of Inflammation | IL-4 Inhibits IL-1β-Induced Depressive-Like Behavior and Central Neurotransmitter Alterations [PubMed: 26417153] |
| Neurochem Res | Protective Effect of Oral Hesperetin Against Unilateral Striatal 6-Hydroxydopamine Damage in the Rat [PubMed: 26700436] |
| Cell Mol Neurobiol | Hypericum Perforatum Hydroalcoholic Extract Mitigates Motor Dysfunction and is Neuroprotective in Intrastriatal 6-Hydroxydopamine Rat Model of Parkinson’s Disease [PubMed: 26119304] |
| Sleep and Biological Rhythms | Relationship of orexin (hypocretin) system and astrocyte activation in Parkinson's disease with hypersomnolence [Doi: 10.1111] |
| Cellular and Molecular Neurobiology | Hypericum Perforatum?Hydroalcoholic Extract Mitigates Motor Dysfunction and is Neuroprotective in Intrastriatal 6-Hydroxydopamine Rat Model of Parkinson’s Disease [Pubmed:26119304] |
| Ann Anat | Effect of memantine: A NMDA receptor blocker, on ethambutol-induced retinal injury [Pubmed:26704355] |
| United States Patent Application | ESTROGENIC COMPONENTS FOR USE IN THE TREATMENT OF NEUROLOGICAL DISORDERS [y2016:0101116.html] |
| Clinical Research | Human plasma glial fibrillary acidic protein and heat shock protein 27 concentrations in acute moderate-intensity aerobic exercise with different duration [a825e1e27b0221df9a627769c0f614432e92.pdf] |
| Medical Journal of Indonesia | Comparison of GFAP and HSP27 concentrations in acute moderate-intensity aerobic exercise of different duration [2031884] |
| Peptides. | Carnosine ameliorates cognitive deficits in streptozotocin-induced diabetic rats: Possible involved mechanisms. [pubmed:27777064] |
| european journal of pharmacology | Garlic active constituent s-allyl cysteine protects against lipopolysaccharide-induced cognitive deficits in the rat: Possible involved mechanisms. [pubmed:27915041] |
| european journal of pharmacology | Troxerutin exerts neuroprotection in 6-hydroxydopamine lesion rat model of Parkinson's disease: Possible involvement of PI3K/ERβ signaling. [pubmed:28284752] |
| biomedicine & pharmacotherapy | Acetyl-l-carnitine protects dopaminergic nigrostriatal pathway in 6-hydroxydopamine-induced model of Parkinson's disease in the rat. [pubmed:28199883] |
| J Endocrinol.? | Estrogen receptors and estetrol-dependent neuroprotective actions: a pilot study. [pubmed:27799463] |
| IOS Press Content Library | An Intranasal Formulation of Erythropoietin (Neuro-EPO) Prevents Memory Deficits and Amyloid Toxicity in the APPSwe Transgenic Mouse Model of Alzheimer's … [articles:journal-of-alzheimers-disease] |
| Tier?rztliche Hochschule Hannover | Evaluierung von Biomarkern im Liquor cerebrospinalis bei paraplegischen Hunden mit Ru?ckenmarksverletzung [:] |
| Nigerian Journal of Clinical Practice | Utilization of Glial Fibrillary Acidic Protein and Galectin-3 in the Diagnosis of Cerebral Infarction Patients with Normal Cranial Tomography [pubmed:28406123] |
| Cytokine | Soy isoflavone genistein attenuates lipopolysaccharide-induced cognitive impairments in the rat via exerting anti-oxidative and anti-inflammatory effects [pubmed:29102164] |
| Biomedicine & pharmacotherapy | Berberine ameliorates intrahippocampal kainate-induced status epilepticus and consequent epileptogenic process in the rat: Underlying mechanisms [pubmed:28061403] |
| Experimental gerontology | The anti-aging protein klotho alleviates injury of nigrostriatal dopaminergic pathway in 6-hydroxydopamine rat model of Parkinson's disease: Involvement of PKA/CaMKII/CREB signaling. [pubmed:29107062] |
| Pesticide Biochemistry and Physiology | Acrolein-mediated neurotoxicity in growing Wistar male rats [Pubmed:30033014] |
| Journal of Perinatal Medicine | Mechanisms of death in structurally normal stillbirths [Pubmed: 30231013] |
| Metabolic?Brain?Disease | Berberine ameliorates lipopolysaccharide-induced learning and memory deficit in the rat: insights into underlying molecular mechanisms [Pubmed: 30456649] |
| Metabolic?Brain?Disease | Trigonelline protects hippocampus against intracerebral Aβ (1–40) as a model of Alzheimer's disease in the rat: insights into underlying mechanisms [Pubmed: 30421246] |
| 博士論文 | INSTITUTO DE CIENCIAS BáSICAS Y PRECLíNICAS “VICTORIA DE GIRóN” DEPARTAMENTO DE HISTOLOGíA [] |
| Medicina | The Administration of the New Pyrimidine Derivative—4-{2-[2-(3, 4-Dimethoxyphenyl)-Vinyl]-6-Ethyl-4-Oxo-5-Phenyl-4H-Pyrimidine-1-Il} Benzsulfamide Restores the?… [] |
| Open Access Macedonian Journal of Medical Sciences | Inhibition of Receptor for Advanced Glycation End Products as New Promising Strategy Treatment in Diabetic Retinopathy [Pubmed: 32165929] |
| NEUROPSYCHOBIOLOGY | Diosgenin Attenuates Cognitive Impairment in Streptozotocin-Induced Diabetic Rats: Underlying Mechanisms [Pubmed: 32526752] |
| JAMA Surgery | Intraoperative dexmedetomidine for prevention of postoperative delirium in elderly patients with mild cognitive impairment [Pubmed: 28593326] |
| Acta Histochemica | Quercetin mitigates monosodium glutamate-induced excitotoxicity of the spinal cord motoneurons in aged rats via p38 MAPK inhibition [Pubmed: 32622428] |
| stanbul Med J | The Predictive Role of Neurobiochemical Markers in Multiple Sclerosis. [] |
| Basic and Clinical Neuroscience | Paeonol Protects Against Intrastriatal 6-Hydroxydopamine Rat Model of Parkinson's Disease [33995926] |
| pharmacology online | PHARMACOLOGICAL SCREENING OF A POTENTIALLY EFFECTIVE COMPOUND FOR THE TREATMENT OF CHRONIC TRAUMATIC ENCEPHALOPATHY??- [] |
| Neuropeptides | The bee venom active compound melittin protects against bicuculline-induced seizures and hippocampal astrocyte activation in rats [34808488] |
| Neural Plast | P2Y2 Receptor Mediated Neuronal Regeneration and Angiogenesis to Affect Functional Recovery in Rats with Spinal Cord Injury [Pubmed:35154311] |
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