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視黃醇結(jié)合蛋白4(RBP4)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)

ELISA Kit for Retinol Binding Protein 4 (RBP4)

PRBP; RBP; Plasma retinol-binding protein; Retinol Binding Protein 4, Plasma

  • 視黃醇結(jié)合蛋白4(RBP4)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) 產(chǎn)品包裝(模擬)
  • 視黃醇結(jié)合蛋白4(RBP4)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) 產(chǎn)品包裝(模擬)
  • 視黃醇結(jié)合蛋白4(RBP4)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) 實(shí)驗(yàn)結(jié)果圖
  • SEA929Mu.jpg 標(biāo)準(zhǔn)曲線圖
  • Certificate 通過(guò)ISO 9001、ISO 13485質(zhì)量體系認(rèn)證

特異性

本試劑盒用于檢測(cè)視黃醇結(jié)合蛋白4(RBP4),經(jīng)檢測(cè)與其它相似物質(zhì)無(wú)明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來(lái)源的限制,不可能完成對(duì)所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測(cè),因此本試劑盒有可能與未經(jīng)檢測(cè)的其它物質(zhì)有交叉反應(yīng)。

精密度

精密度用樣品測(cè)定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差:取同批次試劑盒對(duì)低、中、高值定值樣本進(jìn)行定量檢測(cè),每份樣本連續(xù)測(cè)定20 次,分別計(jì)算不同濃度樣本的平均值及SD值。
批間差:選取3個(gè)不同批次的試劑盒分別對(duì)低、中、高值定值樣本進(jìn)行定量測(cè)定,每個(gè)樣本使用同一試劑盒重復(fù)測(cè)定8次,分別計(jì)算不同濃度樣本的平均值及SD值。
批內(nèi)差: CV<10%
批間差: CV<12%

穩(wěn)定性

經(jīng)測(cè)定,試劑盒在有效期內(nèi)按推薦溫度保存,其活性降低率小于5%。
為減小外部因素對(duì)試劑盒破壞前后檢測(cè)值的影響,實(shí)驗(yàn)室的環(huán)境條件需盡量保持一致,尤其是實(shí)驗(yàn)室內(nèi)溫度、濕度及溫育條件。其次由同一實(shí)驗(yàn)員來(lái)進(jìn)行操作可減少人為誤差。

實(shí)驗(yàn)流程

1. 實(shí)驗(yàn)前標(biāo)準(zhǔn)品、試劑及樣本的準(zhǔn)備;
2. 加樣(標(biāo)準(zhǔn)品及樣本)100µL,37°C孵育1小時(shí);
3. 吸棄,加檢測(cè)溶液A100µL,37°C孵育1小時(shí);
4. 洗板3次;
5. 加檢測(cè)溶液B100µL,37°C孵育30分鐘;
6. 洗板5次;
7. 加TMB底物90µL,37°C孵育10-20分鐘;
8. 加終止液50µL,立即450nm讀數(shù)。

實(shí)驗(yàn)原理

將視黃醇結(jié)合蛋白4(RBP4)抗體包被于96孔微孔板中,制成固相載體,向微孔中分別加入標(biāo)準(zhǔn)品或標(biāo)本,其中的視黃醇結(jié)合蛋白4(RBP4)與連接于固相載體上的抗體結(jié)合,然后加入生物素化的視黃醇結(jié)合蛋白4(RBP4)抗體,將未結(jié)合的生物素化抗體洗凈后,加入HRP標(biāo)記的親和素,再次徹底洗滌后加入TMB底物顯色。TMB在過(guò)氧化物酶的催化下轉(zhuǎn)化成藍(lán)色,并在酸的作用下轉(zhuǎn)化成最終的黃色。顏色的深淺和樣品中的視黃醇結(jié)合蛋白4(RBP4)呈正相關(guān)。用酶標(biāo)儀在450nm波長(zhǎng)下測(cè)定吸光度(O.D.值),計(jì)算樣品濃度。

相關(guān)產(chǎn)品

編號(hào) 適用物種:Mus musculus (Mouse,小鼠) 應(yīng)用(僅供研究使用,不用于臨床診斷!)
RPA929Mu01 視黃醇結(jié)合蛋白4(RBP4)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APA929Mu01 視黃醇結(jié)合蛋白4(RBP4)活性蛋白 Cell?culture;?Activity?Assays.
RPA929Mu02 視黃醇結(jié)合蛋白4(RBP4)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAA929Mu02 視黃醇結(jié)合蛋白4(RBP4)多克隆抗體 WB; IHC; ICC; IP.
PAA929Mu01 視黃醇結(jié)合蛋白4(RBP4)多克隆抗體 WB; IHC; ICC; IP.
FAA929Mu01 抗視黃醇結(jié)合蛋白4(RBP4)多克隆抗體 Flow cytometry.
LAA929Mu81 視黃醇結(jié)合蛋白4(RBP4)多克隆抗體(異硫氰酸熒光素標(biāo)記) WB; IHC; ICC; IF.
LAA929Mu71 視黃醇結(jié)合蛋白4(RBP4)多克隆抗體(生物素標(biāo)記) WB; IHC; ICC.
SEA929Mu 視黃醇結(jié)合蛋白4(RBP4)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA929Mu 視黃醇結(jié)合蛋白4(RBP4)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) FLIA Kit for Antigen Detection.
KSA929Mu01 視黃醇結(jié)合蛋白4(RBP4)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) Main materials for "Do It (ELISA Kit) Yourself".

參考文獻(xiàn)

雜志 參考文獻(xiàn)
Diabetes Technology & Therapeutics Proteomic Identification of Human Urinary Biomarkers in Diabetes Mellitus Type 2 [Liebert: 20100078]
Journal of Pharmaceutical and Biomedical Analysis Effect of high dose thiamine on the levels of urinary protein biomarkers in diabetes mellitus type 2 [PubMed: 21130593]
Evid Based Complement Alternat Med Quercetin Protects against Cadmium-Induced Renal Uric Acid Transport System Alteration and Lipid Metabolism Disorder in Rats [PubMed: PMC3368504]
Journal of Endocrinology Combined galanin with insulin improves insulin sensitivity of diabetic rat muscles [Pubmed:24501381]
J Endocrinol Central alarin ameliorated insulin resistance of adipocytes in type 2 diabetic rats [Pubmed:25240061]
Peptides Effect of endogenous galanin on glucose transporter 4 expression in cardiac muscle of type 2 diabetic rats [Pubmed:25445608]
Obesity Surgery Long-term Effect of Ileal Transposition on Adipokine Serum Level in Zucker (Orl)-Lepr fa Fatty Rats [Pubmed:25697126]
Clin Exp Nephrol Differentially expressed urinary biomarkers in children with idiopathic nephrotic syndrome [PubMed: 26351173]
Experimental and Therapeutic Medicine Expression of retinol binding protein 4 and nuclear factor-??B in diabetic rats with atherosclerosis and the intervention effect of pioglitazone [Pubmed:27446311]
Experimental and Therapeutic Medicine Expression?of?retinol?binding?protein?4?and?nuclear?factor-κB in?diabetic?rats?with?atherosclerosisand the?intervention?effect?of?pioglitazone. [pubmed:27446311]
Journal of Chromatography A Application of a new procedure for liquid chromatography/mass spectrometry profiling of plasma amino acid-related metabolites and untargeted shotgun proteomics to identify mechanisms and biomarkers of calcific aortic stenosis [S0021967317311858]
BMC Cancer Screening for immune-potentiating antigens from hepatocellular carcinoma patients after radiofrequency ablation by serum proteomic analysis [Pubmed:29386009]
Nutrition &?Diabetes Lack of pronounced changes in the expression of fatty acid handling proteins in adipose tissue and plasma of morbidly obese humans [Pubmed:29335416]
Molecular?Medicine?Reports RBP4 regulates trophoblastic cell proliferation and invasion via the PI3K/AKT signaling pathway [Pubmed:30015949]
Relationship between retinol and risk of diabetic retinopathy: a case-control study []
Scientific Reports Retinol binding protein 4 abundance in plasma and tissues is related to body fat deposition in cattle [Pubmed: 31147589]
Potassium supplementation blunts the effects of high salt intake on serum retinol‐binding protein 4 levels in healthy individuals []
Docosahexaenoic acid (DHA), a nutritional supplement, modulates steroid insensitivity in asthma []
Eur J Nutr The dietary inflammatory index is associated with anti-and pro-inflammatory adipokines in Brazilian schoolchildren [33575861]
Eur J Pediatr Pro-and anti-inflammatory adipokines are associated with cardiometabolic risk markers in Brazilian schoolchildren [33834274]
Sci Rep Ileal transposition helps to regulate plasma hepatokine levels in obese Zucker (Crl: ZUC (ORL)-Lepr fa) rats [33833309]
Int Arch Occup Environ Health Biomarkers of cadmium exposure and renal function in estuarine adult villagers [34773507]
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