補體成分3(C3)檢測試劑盒(酶聯(lián)免疫吸附試驗法)
ELISA Kit for Complement Component 3 (C3)
ASP; CPAMD1; Complement Protein C3; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1; C3a anaphylatoxin; Acylation stimulating protein
- 編號SEA861Po
- 物種Sus scrofa; Porcine (Pig,豬) 相同的名稱,不同的物種。
- 實驗方法雙抗夾心
- 反應時長3h
- 檢測范圍3.12-200ng/mL
- 靈敏度最小可檢測劑量小于等于1.33ng/mL.
- 樣本類型Serum, plasma and other biological fluids
- 下載 英文說明書 中文說明書
- 規(guī)格 48T96T 96T*5 96T*10 96T*100
- 價格 ¥ 3175 ¥ 4536 ¥ 20412 ¥ 38556 ¥ 317520
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特異性
本試劑盒用于檢測補體成分3(C3),經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應。
由于受到技術(shù)及樣本來源的限制,不可能完成對所有相關(guān)或相似物質(zhì)交叉反應檢測,因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應。
回收率
分別于定值血清及血漿樣本中加入一定量的補體成分3(C3)(加標樣品),重復測定并計算其均值,回收率為測定值與理論值的比率。
樣本 | 回收率范圍(%) | 平均回收率(%) |
serum(n=5) | 92-101 | 95 |
EDTA plasma(n=5) | 98-105 | 102 |
heparin plasma(n=5) | 78-94 | 88 |
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差:取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次,分別計算不同濃度樣本的平均值及SD值。
批間差:選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定,每個樣本使用同一試劑盒重復測定8次,分別計算不同濃度樣本的平均值及SD值。
批內(nèi)差: CV<10%
批間差: CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的補體成分3(C3),并倍比稀釋成1:2,1:4,1:8,1:16的待測樣本,線性范圍即為稀釋后樣本中補體成分3(C3)含量的測定值與理論值的比率。
樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
serum(n=5) | 99-105% | 98-105% | 82-90% | 91-98% |
EDTA plasma(n=5) | 89-102% | 80-94% | 87-101% | 78-90% |
heparin plasma(n=5) | 89-99% | 89-103% | 91-98% | 82-103% |
穩(wěn)定性
經(jīng)測定,試劑盒在有效期內(nèi)按推薦溫度保存,其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響,實驗室的環(huán)境條件需盡量保持一致,尤其是實驗室內(nèi)溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。
實驗流程
1. 實驗前標準品、試劑及樣本的準備;
2. 加樣(標準品及樣本)100µL,37°C孵育1小時;
3. 吸棄,加檢測溶液A100µL,37°C孵育1小時;
4. 洗板3次;
5. 加檢測溶液B100µL,37°C孵育30分鐘;
6. 洗板5次;
7. 加TMB底物90µL,37°C孵育10-20分鐘;
8. 加終止液50µL,立即450nm讀數(shù)。
實驗原理
將補體成分3(C3)抗體包被于96孔微孔板中,制成固相載體,向微孔中分別加入標準品或標本,其中的補體成分3(C3)與連接于固相載體上的抗體結(jié)合,然后加入生物素化的補體成分3(C3)抗體,將未結(jié)合的生物素化抗體洗凈后,加入HRP標記的親和素,再次徹底洗滌后加入TMB底物顯色。TMB在過氧化物酶的催化下轉(zhuǎn)化成藍色,并在酸的作用下轉(zhuǎn)化成最終的黃色。顏色的深淺和樣品中的補體成分3(C3)呈正相關(guān)。用酶標儀在450nm波長下測定吸光度(O.D.值),計算樣品濃度。
增值服務
相關(guān)產(chǎn)品
編號 | 適用物種:Sus scrofa; Porcine (Pig,豬) | 應用(僅供研究使用,不用于臨床診斷!) |
RPA861Po01 | 補體成分3(C3)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
PAA861Po01 | 補體成分3(C3)多克隆抗體 | WB; IHC; ICC; IP. |
MAA861Po21 | 補體成分3(C3)單克隆抗體 | WB; IHC; ICC; IP. |
SEA861Po | 補體成分3(C3)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
LMA861Po | 補體成分3(C3)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
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