P選擇素(SELP)檢測試劑盒(酶聯(lián)免疫吸附試驗法)
ELISA Kit for P-Selectin (SELP)
CD62P; Selectin, Platelet; PSEL; GMP140; LECAM3; GRMP; GMRP; PADGEM; Leukocyte-endothelial cell adhesion molecule 3; Platelet activation dependent granule-external membrane
- 編號SEA569Rb
- 物種Oryctolagus cuniculus (Rabbit，兔) 相同的名稱，不同的物種。
- 實驗方法雙抗夾心
- 反應時長3h
- 檢測范圍1.56-100ng/mL
- 靈敏度最小可檢測劑量小于等于0.55ng/mL.
- 樣本類型Serum, plasma, tissue homogenates and other biological fluids
- 下載 英文說明書 中文說明書
- 規(guī)格 48T96T 96T*5 96T*10 96T*100
- 價格 ¥ 2873 ¥ 4104 ¥ 18468 ¥ 34884 ¥ 287280
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產品包裝(模擬)
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產品包裝(模擬)
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實驗結果圖
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標準曲線圖
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通過ISO 9001、ISO 13485質量體系認證
特異性
本試劑盒用于檢測P選擇素(SELP)，經檢測與其它相似物質無明顯交叉反應。
由于受到技術及樣本來源的限制，不可能完成對所有相關或相似物質交叉反應檢測，因此本試劑盒有可能與未經檢測的其它物質有交叉反應。
回收率
分別于定值血清及血漿樣本中加入一定量的P選擇素(SELP)（加標樣品），重復測定并計算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 80-96 | 89 |
| EDTA plasma(n=5) | 82-101 | 88 |
| heparin plasma(n=5) | 86-104 | 99 |
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內差：取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定，每個樣本使用同一試劑盒重復測定8次，分別計算不同濃度樣本的平均值及SD值。
批內差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內加入適量的P選擇素(SELP)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中P選擇素(SELP)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 98-105% | 81-92% | 78-104% | 87-105% |
| EDTA plasma(n=5) | 88-96% | 83-101% | 98-105% | 92-101% |
| heparin plasma(n=5) | 86-95% | 87-104% | 80-97% | 96-103% |
穩(wěn)定性
經測定，試劑盒在有效期內按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。
實驗流程
1. 實驗前標準品、試劑及樣本的準備；
2. 加樣（標準品及樣本）100µL，37°C孵育1小時；
3. 吸棄，加檢測溶液A100µL，37°C孵育1小時；
4. 洗板3次；
5. 加檢測溶液B100µL，37°C孵育30分鐘；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分鐘；
8. 加終止液50µL，立即450nm讀數(shù)。
實驗原理
將P選擇素(SELP)抗體包被于96孔微孔板中，制成固相載體，向微孔中分別加入標準品或標本，其中的P選擇素(SELP)與連接于固相載體上的抗體結合，然后加入生物素化的P選擇素(SELP)抗體，將未結合的生物素化抗體洗凈后，加入HRP標記的親和素，再次徹底洗滌后加入TMB底物顯色。TMB在過氧化物酶的催化下轉化成藍色，并在酸的作用下轉化成最終的黃色。顏色的深淺和樣品中的P選擇素(SELP)呈正相關。用酶標儀在450nm波長下測定吸光度(O.D.值)，計算樣品濃度。
增值服務
相關產品
| 編號 | 適用物種：Oryctolagus cuniculus (Rabbit，兔) | 應用(僅供研究使用，不用于臨床診斷！) |
| RPA569Rb01 | P選擇素(SELP)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA569Rb51 | P選擇素(SELP)多克隆抗體 | WB; IHC; ICC; IP. |
| PAA569Rb01 | P選擇素(SELP)多克隆抗體 | WB; IHC; ICC; IP. |
| MAA569Rb21 | P選擇素(SELP)單克隆抗體 | WB; IHC; ICC; IP. |
| SEA569Rb | P選擇素(SELP)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA569Rb | P選擇素(SELP)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻
| 雜志 | 參考文獻 |
| Journalof Vascular Research | Effect of Hirulog-Like Peptide on Balloon Catheter Injury-Induced Neointimal Formation in Femoral Arteries of Minipigs and Relationship with Inflammatory Mediators [Karger: 000257340] |
| Basic Research in Cardiology | Secretome of apoptotic peripheral blood cells (APOSEC) attenuates microvascular obstruction in a porcine closed chest reperfused acute myocardial infarction model: role of platelet aggregation and vasodilation [PubMed: 22899170] |
| BioMed research international | Endothelial gene expression and molecular changes in response to radiosurgery in in vitro and in vivo models of cerebral arteriovenous malformations [Pubmed: 24199192] |
| Free Radical Biology and Medicine | Involvement of nitric oxide with activation of Toll-like receptor 4 signaling in mice with dextran sodium sulfate-induced colitis [Pubmed:24992835] |
| Materials Science and Engineering: C | MRI-compatible Nb–60Ta–2Zr alloy used for vascular stents: Haemocompatibility and its correlation with protein adsorption [Pubmed:25063132] |
| Blood Coagul Fibrinolysis.? | Chemical sympathectomy attenuates inflammation, glycocalyx shedding and coagulation disorders in rats with acute traumatic coagulopathy. [Pubmed:25325345] |
| Inflammation.? | Systemic and Flap Inflammatory Response Associates with Thrombosis in Flap Venous Crisis [Pubmed:25448261] |
| Endocrine | Effects of glucagon-like peptide-1 on advanced glycation endproduct-induced aortic endothelial dysfunction in streptozotocin-induced diabetic rats: possible roles of Rho kinase- and AMP kinase-mediated nuclear factor κB signaling pathways [Pubmed:26758998] |
| Microbial Pathogenesis | Protective effects of sinomenine against LPS-induced inflammation in piglets. [pubmed:28757275] |
| Nutrition?&?Metabolism | L-Carnitine Supplementation Increases Trimethylamine-N-Oxide but not Markers of Atherosclerosis in Healthy Aged Women [Pubmed: 30485835] |
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| JOURNAL OF CONTROLLED RELEASE | Polycaprolactone vascular graft with epigallocatechin gallate embedded sandwiched layer-by-layer functionalization for enhanced antithrombogenicity and anti … [Pubmed: 31982435] |
| OXIDATIVE MEDICINE AND CELLULAR LONGEVITY | The Inhibition of P-Selectin Reduced Severe Acute Lung Injury in Immunocompromised Mice [Pubmed: 32377309] |
| blood advances | The binding of autotaxin to integrins mediates hyperhomocysteinemia-potentiated platelet activation and thrombosis [34559203] |
| Vaccine | The effect of ChAdOx1 nCov-19 vaccine on arterial thrombosis development and platelet aggregation in female rats [Pubmed:35183388] |
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| Experimental and Therapeutic Medicine | Construction of a rabbit model with vinorelbine administration via peripherally inserted central catheter and dynamic monitoring of changes in phlebitis and thrombosis [Pubmed:35126715] |
