組織因子(TF)檢測試劑盒(酶聯(lián)免疫吸附試驗法)
ELISA Kit for Tissue Factor (TF)
CD142; FIII; F3; TFA; Thromboplastin; Coagulation Factor III
- 編號SEA524Rb
- 物種Oryctolagus cuniculus (Rabbit，兔) 相同的名稱，不同的物種。
- 實驗方法雙抗夾心
- 反應(yīng)時長3h
- 檢測范圍6.25-400pg/mL
- 靈敏度最小可檢測劑量小于等于2.63pg/mL.
- 樣本類型plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下載 英文說明書 中文說明書
- 規(guī)格 48T96T 96T*5 96T*10 96T*100
- 價格 ¥ 2873 ¥ 4104 ¥ 18468 ¥ 34884 ¥ 287280
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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實驗結(jié)果圖
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標(biāo)準(zhǔn)曲線圖
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通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
本試劑盒用于檢測組織因子(TF)，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來源的限制，不可能完成對所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應(yīng)。
回收率
分別于定值血清及血漿樣本中加入一定量的組織因子(TF)（加標(biāo)樣品），重復(fù)測定并計算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| sodium citrate plasma(n=5) | 89-105 | 98 |
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進(jìn)行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進(jìn)行定量測定，每個樣本使用同一試劑盒重復(fù)測定8次，分別計算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的組織因子(TF)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中組織因子(TF)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| sodium citrate plasma(n=5) | 88-104% | 90-98% | 85-96% | 94-102% |
穩(wěn)定性
經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內(nèi)溫度、濕度及溫育條件。其次由同一實驗員來進(jìn)行操作可減少人為誤差。
實驗流程
1. 實驗前標(biāo)準(zhǔn)品、試劑及樣本的準(zhǔn)備；
2. 加樣（標(biāo)準(zhǔn)品及樣本）100µL，37°C孵育1小時；
3. 吸棄，加檢測溶液A100µL，37°C孵育1小時；
4. 洗板3次；
5. 加檢測溶液B100µL，37°C孵育30分鐘；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分鐘；
8. 加終止液50µL，立即450nm讀數(shù)。
實驗原理
將組織因子(TF)抗體包被于96孔微孔板中，制成固相載體，向微孔中分別加入標(biāo)準(zhǔn)品或標(biāo)本，其中的組織因子(TF)與連接于固相載體上的抗體結(jié)合，然后加入生物素化的組織因子(TF)抗體，將未結(jié)合的生物素化抗體洗凈后，加入HRP標(biāo)記的親和素，再次徹底洗滌后加入TMB底物顯色。TMB在過氧化物酶的催化下轉(zhuǎn)化成藍(lán)色，并在酸的作用下轉(zhuǎn)化成最終的黃色。顏色的深淺和樣品中的組織因子(TF)呈正相關(guān)。用酶標(biāo)儀在450nm波長下測定吸光度(O.D.值)，計算樣品濃度。
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Oryctolagus cuniculus (Rabbit，兔) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| RPA524Rb02 | 組織因子(TF)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA524Rb01 | 組織因子(TF)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA524Rb52 | 組織因子(TF)多克隆抗體 | WB; IHC; ICC; IP. |
| PAA524Rb51 | 組織因子(TF)多克隆抗體 | WB; IHC; ICC; IP. |
| PAA524Rb01 | 組織因子(TF)多克隆抗體 | WB; IHC; ICC; IP. |
| LAA524Rb71 | 組織因子(TF)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| MAA524Rb21 | 組織因子(TF)單克隆抗體 | WB; IHC; ICC; IP. |
| SEA524Rb | 組織因子(TF)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA524Rb | 組織因子(TF)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| PSA524Rb01 | 組織因子(TF)抗體對 | ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays. |
| KSA524Rb01 | 組織因子(TF)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
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| BioMed research international | Endothelial gene expression and molecular changes in response to radiosurgery in in vitro and in vivo models of cerebral arteriovenous malformations [Pubmed: 24199192] |
| The Journal of Biological Chemistry | Sphingolipid Pathway Regulates Innate Immune Responses at the Fetomaternal Interface during Pregnancy [Pubmed:25505239] |
| Hippokratia | Thrombosis in Hemodialysis Patients; Their Association with Tissue Factor and Tissue Factor Pathway Inhibitor [PubMed: 20011089] |
| shock | Noble-Collip Drum Trauma Induces Disseminated Intravascular Coagulation But Not Acute Coagulopathy of Trauma-Shock [PubMed: 25423126] |
| Perfusion | Ex vivo simulation of cardiopulmonary bypass with human blood for hemocompatibility testing [PubMed: 26243277] |
| Molecular Nutrition & Food Research | Trans‐fatty acid promotes thrombus formation in mice by aggravating antithrombogenic endothelial functions via Toll‐like receptors [PubMed: 25546502] |
| Food Funct | The effects of polysaccharides from the root of Angelica sinensis on tumor growth and iron metabolism in H22-bearing mice [Pubmed:26757699] |
| Blood Cells, Inflammation and Infection | Nebulized Heparin Attenuates Pulmonary Coagulopathy and Inflammation through Alveolar Macrophages in a Rat Model of Acute Lung Injury [pubmed:29202212] |
| Thrombosis?andHaemostasis | Nebulized anti-coagulants as a therapy for acute lung injury and acute respiratory distress syndrome [Pubmed: 29202212] |
| Journal of Thrombosis and Haemostasis | Effects of nebulized antithrombin and heparin on inflammatory and coagulation alterations in an acute lung injury model in rats [Pubmed: 31755229] |
| Mediators Inflamm | Amelioration of Coagulation Disorders and Inflammation by Hydrogen-Rich Solution Reduces Intestinal Ischemia/Reperfusion Injury in Rats through NF-κB … [Pubmed: 32587471] |
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