軸突生長誘向因子1(Ntn1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)
ELISA Kit for Netrin 1 (Ntn1)
NTN1L; Epididymis tissue protein Li 131P
- 編號SEB827Ra
- 物種Rattus norvegicus (Rat，大鼠) 相同的名稱，不同的物種。
- 實驗方法雙抗夾心
- 反應(yīng)時長3h
- 檢測范圍15.6-1,000pg/mL
- 靈敏度最小可檢測劑量小于等于5.7pg/mL.
- 樣本類型Serum, plasma, tissue homogenates and other biological fluids
- 下載 英文說明書 中文說明書
- 規(guī)格 48T96T 96T*5 96T*10 96T*100
- 價格 ¥ 3032 ¥ 4332 ¥ 19494 ¥ 36822 ¥ 303240
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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實驗結(jié)果圖
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標(biāo)準(zhǔn)曲線圖
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通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
本試劑盒用于檢測軸突生長誘向因子1(Ntn1)，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來源的限制，不可能完成對所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應(yīng)。
回收率
分別于定值血清及血漿樣本中加入一定量的軸突生長誘向因子1(Ntn1)（加標(biāo)樣品），重復(fù)測定并計算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 86-98 | 92 |
| EDTA plasma(n=5) | 96-103 | 101 |
| heparin plasma(n=5) | 79-101 | 83 |
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進(jìn)行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進(jìn)行定量測定，每個樣本使用同一試劑盒重復(fù)測定8次，分別計算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的軸突生長誘向因子1(Ntn1)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中軸突生長誘向因子1(Ntn1)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 99-105% | 78-103% | 82-91% | 87-94% |
| EDTA plasma(n=5) | 85-101% | 78-104% | 78-102% | 88-101% |
| heparin plasma(n=5) | 94-101% | 86-103% | 87-101% | 80-98% |
穩(wěn)定性
經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內(nèi)溫度、濕度及溫育條件。其次由同一實驗員來進(jìn)行操作可減少人為誤差。
實驗流程
1. 實驗前標(biāo)準(zhǔn)品、試劑及樣本的準(zhǔn)備；
2. 加樣（標(biāo)準(zhǔn)品及樣本）100µL，37°C孵育1小時；
3. 吸棄，加檢測溶液A100µL，37°C孵育1小時；
4. 洗板3次；
5. 加檢測溶液B100µL，37°C孵育30分鐘；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分鐘；
8. 加終止液50µL，立即450nm讀數(shù)。
實驗原理
將軸突生長誘向因子1(Ntn1)抗體包被于96孔微孔板中，制成固相載體，向微孔中分別加入標(biāo)準(zhǔn)品或標(biāo)本，其中的軸突生長誘向因子1(Ntn1)與連接于固相載體上的抗體結(jié)合，然后加入生物素化的軸突生長誘向因子1(Ntn1)抗體，將未結(jié)合的生物素化抗體洗凈后，加入HRP標(biāo)記的親和素，再次徹底洗滌后加入TMB底物顯色。TMB在過氧化物酶的催化下轉(zhuǎn)化成藍(lán)色，并在酸的作用下轉(zhuǎn)化成最終的黃色。顏色的深淺和樣品中的軸突生長誘向因子1(Ntn1)呈正相關(guān)。用酶標(biāo)儀在450nm波長下測定吸光度(O.D.值)，計算樣品濃度。
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Rattus norvegicus (Rat，大鼠) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| RPB827Ra01 | 軸突生長誘向因子1(Ntn1)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAB827Ra01 | 軸突生長誘向因子1(Ntn1)多克隆抗體 | WB; IHC; ICC; IP. |
| MAB827Ra21 | 軸突生長誘向因子1(Ntn1)單克隆抗體 | WB; IHC; ICC; IP. |
| SEB827Ra | 軸突生長誘向因子1(Ntn1)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMB827Ra | 軸突生長誘向因子1(Ntn1)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| Transplantation Proceedings | Netrin-1: A Novel Universal Biomarker of Human Kidney Injury [PubMed: 20620466] |
| Biomarkers | Plasma netrin-1 is a diagnostic biomarker of human cancers [PubMed: PMC3143477] |
| Journal of Maternal-Fetal and Neonatal Medicine | Cord blood netrin-1 and -4 concentrations in term pregnancies with normal, restricted and increased fetal growth [Informahealthcare: Source] |
| 4 | Netrin-1 promotes adipose tissue macrophage retention and insulin resistance in obesity [Pubmed:24584118] |
| J Matern?Fetal?Neonatal Med.? | Cord blood netrin-1 and-4 concentrations in term pregnancies with normal, restricted and increased fetal growth [Pubmed:24716747] |
| Renal failure. | Urinary netrin-1 and KIM-1 as early biomarkers for septic acute kidney injury [Pubmed:25154466] |
| Stem Cell Research & Therapy | Netrin-1 acts as a non-canonical angiogenic factor produced by human Wharton’s jelly mesenchymal stem cells (WJ-MSC) [articles:10.1186] |
| Oncotarget | The effect of aspirin on circulating netrin-1 levels in humans is dependent on the inflammatory status of the vascular endothelium. [pubmed:29156815] |
| Frontiers in?Cellular?Neuroscience | Neuroprotection Exerted by Netrin-1 and Kinesin Motor KIF1A in Secondary Brain Injury following Experimental Intracerebral Hemorrhage in Rats [Pubmed:29375318] |
| Scientific Reports | Netrin-1 promotes diabetic corneal wound healing through molecular mechanisms mediated via the adenosine 2B receptor [Pubmed:29662125] |
| Family Practice and Palliative Care | Relationship of serum netrin-1 levels with breast masses [Doi: 10.22391/fppc.467700] |
| JOURNAL OF BIOLOGICAL CHEMISTRY | Role of microRNA in CB1 antagonist-mediated regulation of adipose tissue macrophage polarization and chemotaxis during diet-induced obesity [Pubmed: 30910812] |
| International Journal of Molecular?Sciences | Downregulation of the Netrin-1 Receptor UNC5b Underlies Increased Placental Angiogenesis in Human Gestational Diabetes Mellitus [Pubmed: 30897795] |
| Cancer Biomark | Serum netrin-1 as a biomarker for colorectal cancer detection [Pubmed: 32474463] |
| Níveis Elevados de Netrina-1 e IL-1β em Mulheres Idosas com SCA: Pior Prognóstico no Acompanhamento de Dois Anos [] | |
| Tuberk Toraks | Association of plasma netrin-1 level with inflammatory balance and comorbidities in patients with acute exacerbation of COPD [33853303] |
| Adv Healthc Mater | Conductive Hydrogel Conduits with Growth Factor Gradients for Peripheral Nerve Repair in Diabetics with Non‐suture Tape [Pubmed:35670309] |
