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細胞因子信號轉導抑制因子3(SOCS3)檢測試劑盒(酶聯(lián)免疫吸附試驗法)

ELISA Kit for Suppressors Of Cytokine Signaling 3 (SOCS3)

ATOD4; CIS3; Cish3; MGC71791; SOCS-3; SSI-3; SSI3; Cytokine-inducible SH2 protein 3; STAT-induced STAT inhibitor 3

  • 細胞因子信號轉導抑制因子3(SOCS3)檢測試劑盒(酶聯(lián)免疫吸附試驗法) 產(chǎn)品包裝(模擬)
  • 細胞因子信號轉導抑制因子3(SOCS3)檢測試劑盒(酶聯(lián)免疫吸附試驗法) 產(chǎn)品包裝(模擬)
  • 細胞因子信號轉導抑制因子3(SOCS3)檢測試劑盒(酶聯(lián)免疫吸附試驗法) 實驗結果圖
  • SEB684Mu.jpg 標準曲線圖
  • Certificate 通過ISO 9001、ISO 13485質(zhì)量體系認證

特異性

本試劑盒用于檢測細胞因子信號轉導抑制因子3(SOCS3),經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應。
由于受到技術及樣本來源的限制,不可能完成對所有相關或相似物質(zhì)交叉反應檢測,因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應。

精密度

精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差:取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次,分別計算不同濃度樣本的平均值及SD值。
批間差:選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定,每個樣本使用同一試劑盒重復測定8次,分別計算不同濃度樣本的平均值及SD值。
批內(nèi)差: CV<10%
批間差: CV<12%

穩(wěn)定性

經(jīng)測定,試劑盒在有效期內(nèi)按推薦溫度保存,其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響,實驗室的環(huán)境條件需盡量保持一致,尤其是實驗室內(nèi)溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。

實驗流程

1. 實驗前標準品、試劑及樣本的準備;
2. 加樣(標準品及樣本)100µL,37°C孵育1小時;
3. 吸棄,加檢測溶液A100µL,37°C孵育1小時;
4. 洗板3次;
5. 加檢測溶液B100µL,37°C孵育30分鐘;
6. 洗板5次;
7. 加TMB底物90µL,37°C孵育10-20分鐘;
8. 加終止液50µL,立即450nm讀數(shù)。

實驗原理

將細胞因子信號轉導抑制因子3(SOCS3)抗體包被于96孔微孔板中,制成固相載體,向微孔中分別加入標準品或標本,其中的細胞因子信號轉導抑制因子3(SOCS3)與連接于固相載體上的抗體結合,然后加入生物素化的細胞因子信號轉導抑制因子3(SOCS3)抗體,將未結合的生物素化抗體洗凈后,加入HRP標記的親和素,再次徹底洗滌后加入TMB底物顯色。TMB在過氧化物酶的催化下轉化成藍色,并在酸的作用下轉化成最終的黃色。顏色的深淺和樣品中的細胞因子信號轉導抑制因子3(SOCS3)呈正相關。用酶標儀在450nm波長下測定吸光度(O.D.值),計算樣品濃度。

相關產(chǎn)品

編號 適用物種:Mus musculus (Mouse,小鼠) 應用(僅供研究使用,不用于臨床診斷!)
RPB684Mu02 細胞因子信號轉導抑制因子3(SOCS3)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPB684Mu01 細胞因子信號轉導抑制因子3(SOCS3)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APB684Mu01 細胞因子信號轉導抑制因子3(SOCS3)活性蛋白 Cell?culture;?Activity?Assays.
PAB684Mu02 細胞因子信號轉導抑制因子3(SOCS3)多克隆抗體 WB; IHC; ICC; IP.
PAB684Mu01 細胞因子信號轉導抑制因子3(SOCS3)多克隆抗體 WB; IHC; ICC; IP.
LAB684Mu71 細胞因子信號轉導抑制因子3(SOCS3)多克隆抗體(生物素標記) WB; IHC; ICC.
LAB684Mu81 細胞因子信號轉導抑制因子3(SOCS3)多克隆抗體(異硫氰酸熒光素標記) WB; IHC; ICC; IF.
SEB684Mu 細胞因子信號轉導抑制因子3(SOCS3)檢測試劑盒(酶聯(lián)免疫吸附試驗法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMB684Mu 細胞因子信號轉導抑制因子3(SOCS3)等多因子檢測試劑盒(流式熒光發(fā)光法) FLIA Kit for Antigen Detection.

參考文獻

雜志 參考文獻
The Journal of Biological Chemistry Human primary keratinocytes show a restricted ability to up-regulate suppressor of cytokine signaling (SOCS)3 compared to autologous macrophages [Jcb: 280370]
The Journal of Biological Chemistry Human Primary Keratinocytes Show Restricted Ability to Up-regulate Suppressor of Cytokine Signaling (SOCS)3 Protein Compared with Autologous Macrophages [PubMed: PMC3323061]
Journal of Reproductive Immunology What is the impact of Th1/Th2 ratio, SOCS3, IL17, and IL35 levels in unexplained infertility? [Pubmed: 24368037]
The Journal of Immunology Alveolar Epithelial Cell–Derived Prostaglandin E2?Serves as a Request Signal for Macrophage Secretion of Suppressor of Cytokine Signaling 3 during Innate Inflammation [Pubmed:27183597]
Scientific Reports The FXR Agonist, Obeticholic Acid, Suppresses HCC Proliferation & Metastasis: Role of IL-6/STAT3 Signalling Pathway [pubmed:28970500]
Journal of Immunological Methods Endogenous peroxidases in sputum interfere with horse-radish peroxidase-based ELISAs. [pubmed:29154772]
Turkish?Journal Of?Medical?Sciences The impact of plasma SOCS3 levels and endometrial leukocytes on unexplained infertility [Pubmed:29914245]
Novel trajectories of Bromocriptine antidiabetic action: Leptin-IL-6/JAK2/p-STAT3/SOCS3, p-IR/p-AKT/GLUT4, PPARγ/Adiponectin, Nrf2/PARP-1, and GLP-1 [articles:10.3389]
JCI Insight Alveolar macrophage secretion of vesicular SOCS3 represents a platform for lung cancer therapeutics [Pubmed: 31619584]
European Journal of Pharmacology Olmesartan attenuates type 2 diabetes-associated liver injury: Cross-talk of AGE/RAGE/JNK, STAT3/SCOS3 and RAS signaling pathways [Pubmed: 32067934]
FASEB JOURNAL Resident alveolar macrophage‐derived vesicular SOCS3 dampens allergic airway inflammation [Pubmed: 32030817]
molecular immunology SOCS3-deficient lung epithelial cells uptaking neutrophil-derived SOCS3 worsens lung influenza infection [Pubmed: 32645550]
Experimental and Therapeutic Medicine Changes and clinical significance of serum inflammatory factors in the treatment of pregnancy hypertension syndrome with magnesium sulfate combined with … [Pubmed: 32742411]
NEUROCHEMICAL RESEARCH Modulation of the Nitric Oxide/BH4 Pathway Protects Against Irradiation-Induced Neuronal Damage [33755856]
BIOMEDICINE & PHARMACOTHERAPY Potential contribution of ELISA and LFI Assays to assessment of the oxidative stress condition based on 8-oxodG biomarker [33957564]
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