細胞因子信號轉導抑制因子3(SOCS3)檢測試劑盒(酶聯(lián)免疫吸附試驗法)
ELISA Kit for Suppressors Of Cytokine Signaling 3 (SOCS3)
ATOD4; CIS3; Cish3; MGC71791; SOCS-3; SSI-3; SSI3; Cytokine-inducible SH2 protein 3; STAT-induced STAT inhibitor 3
- 編號SEB684Mu
- 物種Mus musculus (Mouse,小鼠) 相同的名稱,不同的物種。
- 實驗方法雙抗夾心
- 反應時長3h
- 檢測范圍0.156-10ng/mL
- 靈敏度最小可檢測劑量小于等于0.059ng/mL.
- 樣本類型Tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下載 英文說明書 中文說明書
- 規(guī)格 48T96T 96T*5 96T*10 96T*100
- 價格 ¥ 2873 ¥ 4104 ¥ 18468 ¥ 34884 ¥ 287280
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特異性
本試劑盒用于檢測細胞因子信號轉導抑制因子3(SOCS3),經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應。
由于受到技術及樣本來源的限制,不可能完成對所有相關或相似物質(zhì)交叉反應檢測,因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應。
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差:取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次,分別計算不同濃度樣本的平均值及SD值。
批間差:選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定,每個樣本使用同一試劑盒重復測定8次,分別計算不同濃度樣本的平均值及SD值。
批內(nèi)差: CV<10%
批間差: CV<12%
穩(wěn)定性
經(jīng)測定,試劑盒在有效期內(nèi)按推薦溫度保存,其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響,實驗室的環(huán)境條件需盡量保持一致,尤其是實驗室內(nèi)溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。
實驗流程
1. 實驗前標準品、試劑及樣本的準備;
2. 加樣(標準品及樣本)100µL,37°C孵育1小時;
3. 吸棄,加檢測溶液A100µL,37°C孵育1小時;
4. 洗板3次;
5. 加檢測溶液B100µL,37°C孵育30分鐘;
6. 洗板5次;
7. 加TMB底物90µL,37°C孵育10-20分鐘;
8. 加終止液50µL,立即450nm讀數(shù)。
實驗原理
將細胞因子信號轉導抑制因子3(SOCS3)抗體包被于96孔微孔板中,制成固相載體,向微孔中分別加入標準品或標本,其中的細胞因子信號轉導抑制因子3(SOCS3)與連接于固相載體上的抗體結合,然后加入生物素化的細胞因子信號轉導抑制因子3(SOCS3)抗體,將未結合的生物素化抗體洗凈后,加入HRP標記的親和素,再次徹底洗滌后加入TMB底物顯色。TMB在過氧化物酶的催化下轉化成藍色,并在酸的作用下轉化成最終的黃色。顏色的深淺和樣品中的細胞因子信號轉導抑制因子3(SOCS3)呈正相關。用酶標儀在450nm波長下測定吸光度(O.D.值),計算樣品濃度。
增值服務
相關產(chǎn)品
編號 | 適用物種:Mus musculus (Mouse,小鼠) | 應用(僅供研究使用,不用于臨床診斷!) |
RPB684Mu02 | 細胞因子信號轉導抑制因子3(SOCS3)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
RPB684Mu01 | 細胞因子信號轉導抑制因子3(SOCS3)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
APB684Mu01 | 細胞因子信號轉導抑制因子3(SOCS3)活性蛋白 | Cell?culture;?Activity?Assays. |
PAB684Mu02 | 細胞因子信號轉導抑制因子3(SOCS3)多克隆抗體 | WB; IHC; ICC; IP. |
PAB684Mu01 | 細胞因子信號轉導抑制因子3(SOCS3)多克隆抗體 | WB; IHC; ICC; IP. |
LAB684Mu71 | 細胞因子信號轉導抑制因子3(SOCS3)多克隆抗體(生物素標記) | WB; IHC; ICC. |
LAB684Mu81 | 細胞因子信號轉導抑制因子3(SOCS3)多克隆抗體(異硫氰酸熒光素標記) | WB; IHC; ICC; IF. |
SEB684Mu | 細胞因子信號轉導抑制因子3(SOCS3)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
LMB684Mu | 細胞因子信號轉導抑制因子3(SOCS3)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻
雜志 | 參考文獻 |
The Journal of Biological Chemistry | Human primary keratinocytes show a restricted ability to up-regulate suppressor of cytokine signaling (SOCS)3 compared to autologous macrophages [Jcb: 280370] |
The Journal of Biological Chemistry | Human Primary Keratinocytes Show Restricted Ability to Up-regulate Suppressor of Cytokine Signaling (SOCS)3 Protein Compared with Autologous Macrophages [PubMed: PMC3323061] |
Journal of Reproductive Immunology | What is the impact of Th1/Th2 ratio, SOCS3, IL17, and IL35 levels in unexplained infertility? [Pubmed: 24368037] |
The Journal of Immunology | Alveolar Epithelial Cell–Derived Prostaglandin E2?Serves as a Request Signal for Macrophage Secretion of Suppressor of Cytokine Signaling 3 during Innate Inflammation [Pubmed:27183597] |
Scientific Reports | The FXR Agonist, Obeticholic Acid, Suppresses HCC Proliferation & Metastasis: Role of IL-6/STAT3 Signalling Pathway [pubmed:28970500] |
Journal of Immunological Methods | Endogenous peroxidases in sputum interfere with horse-radish peroxidase-based ELISAs. [pubmed:29154772] |
Turkish?Journal Of?Medical?Sciences | The impact of plasma SOCS3 levels and endometrial leukocytes on unexplained infertility [Pubmed:29914245] |
Novel trajectories of Bromocriptine antidiabetic action: Leptin-IL-6/JAK2/p-STAT3/SOCS3, p-IR/p-AKT/GLUT4, PPARγ/Adiponectin, Nrf2/PARP-1, and GLP-1 [articles:10.3389] | |
JCI Insight | Alveolar macrophage secretion of vesicular SOCS3 represents a platform for lung cancer therapeutics [Pubmed: 31619584] |
European Journal of Pharmacology | Olmesartan attenuates type 2 diabetes-associated liver injury: Cross-talk of AGE/RAGE/JNK, STAT3/SCOS3 and RAS signaling pathways [Pubmed: 32067934] |
FASEB JOURNAL | Resident alveolar macrophage‐derived vesicular SOCS3 dampens allergic airway inflammation [Pubmed: 32030817] |
molecular immunology | SOCS3-deficient lung epithelial cells uptaking neutrophil-derived SOCS3 worsens lung influenza infection [Pubmed: 32645550] |
Experimental and Therapeutic Medicine | Changes and clinical significance of serum inflammatory factors in the treatment of pregnancy hypertension syndrome with magnesium sulfate combined with … [Pubmed: 32742411] |
NEUROCHEMICAL RESEARCH | Modulation of the Nitric Oxide/BH4 Pathway Protects Against Irradiation-Induced Neuronal Damage [33755856] |
BIOMEDICINE & PHARMACOTHERAPY | Potential contribution of ELISA and LFI Assays to assessment of the oxidative stress condition based on 8-oxodG biomarker [33957564] |