非神經(jīng)元性烯醇化酶(ENO1)重組蛋白
Recombinant Enolase 1 (ENO1)
NNE; ENO1L1; MPB1; PPH; Alpha Enolase; Enolase 1; Phosphopyruvate hydratase; Plasminogen-binding protein; 2-phospho-D-glycerate hydro-lyase; C-myc promoter-binding
- 編號RPB449Bo01
- 物種Bos taurus; Bovine (Cattle,牛) 相同的名稱,不同的物種。
- 來源原核表達(dá)
- 宿主E.coli
- 內(nèi)毒素水平<1.0EU/µg(LAL法測定)
- 亞細(xì)胞定位細(xì)胞膜, 細(xì)胞質(zhì)
- 預(yù)測分子量48.9kDa
- 實(shí)際分子量48kDa(差異分析請參閱說明書)
- 片段與標(biāo)簽Met1~Lys434 with N-terminal His Tag
- 緩沖液成份20mM Tris, 150mM NaCl緩沖液(pH8.0, 含有1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose和Proclin300)
- 性狀凍干粉
- 純度> 97%
- 等電點(diǎn)6.5
-
應(yīng)用
Positive Control; Immunogen; SDS-PAGE; WB.
如果需要有生物活性的蛋白,請參見活性蛋白。 - 下載 英文說明書 中文說明書
- 規(guī)格 10μg50μg 200μg 1mg 5mg
- 價格 ¥ 1008 ¥ 2520 ¥ 5040 ¥ 15120 ¥ 37800
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序列
用法
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
儲存
避免反復(fù)凍融。2-8°C不超過一個月,-80°C不超過12個月。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定,具體方法如下:在37°C孵育48小時,沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi),在適當(dāng)?shù)臈l件下存儲,損失率低于5%。
增值服務(wù)
相關(guān)產(chǎn)品
編號 | 適用物種:Bos taurus; Bovine (Cattle,牛) | 應(yīng)用(僅供研究使用,不用于臨床診斷!) |
RPB449Bo01 | 非神經(jīng)元性烯醇化酶(ENO1)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
PAB449Bo01 | 非神經(jīng)元性烯醇化酶(ENO1)多克隆抗體 | WB; IHC; ICC; IP. |
LAB449Bo81 | 非神經(jīng)元性烯醇化酶(ENO1)多克隆抗體(異硫氰酸熒光素標(biāo)記) | WB; IHC; ICC; IF. |
LAB449Bo71 | 非神經(jīng)元性烯醇化酶(ENO1)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
參考文獻(xiàn)
雜志 | 參考文獻(xiàn) |
Analytical Chemistry | Diagnostic detection of human lung cancer-associated antigen using a gold nanoparticle-based electrochemical immunosensor [PubMed: 20557064] |
Translational Research | Proteomic analysis of fine-needle aspiration in differential diagnosis of thyroid nodules [Pubmed:27172385] |
Journal of?Proteomics | Putative salivary biomarkers useful to differentiate patients with fibromyalgia [Pubmed:29654921] |
BioMed Research International | The Diagnostic Accuracy of Combined Enolase/Cr, CA125, and CA19-9 in the Detection of Endometriosis [Pubmed: 33062681] |
Cancer Cell International | Bufalin Induced Mitochondrial Dysfunction Promotes Apoptosis of Glioma Cells by Regulating Annexin A2 and DRP1 Proteins [] |
Cancer Cell Int | Bufalin induces mitochondrial dysfunction and promotes apoptosis of glioma cells by regulating Annexin A2 and DRP1 protein expression [34376212] |
Clin Proteomics | Serum proteomics unveil characteristic protein diagnostic biomarkers and signaling pathways in patients with esophageal squamous cell carcinoma [Pubmed:35610567] |
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