纖連蛋白(FN)重組蛋白
Recombinant Fibronectin (FN)
FN1; CIG; FINC; LETS; MSF; GFND2; Anastellin; Migration-Stimulating Factor; Cold-Insoluble Globulin; Large, External, Transformation-Sensitive Protein
- 編號RPA037Ra01
- 物種Rattus norvegicus (Rat,大鼠) 相同的名稱,不同的物種。
- 來源原核表達(dá)
- 宿主E.coli
- 內(nèi)毒素水平<1.0EU/µg(LAL法測定)
- 亞細(xì)胞定位分泌, 細(xì)胞外基質(zhì)
- 預(yù)測分子量24.7kDa
- 實(shí)際分子量25kDa(差異分析請參閱說明書)
- 片段與標(biāo)簽Ser355~Ile558 with N-terminal His Tag
- 緩沖液成份PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
- 性狀凍干粉
- 純度> 97%
- 等電點(diǎn)6.0
-
應(yīng)用
Positive Control; Immunogen; SDS-PAGE; WB.
如果需要有生物活性的蛋白,請參見活性蛋白。 - 下載 英文說明書 中文說明書
- 規(guī)格 10μg50μg 200μg 1mg 5mg
- 價(jià)格 ¥ 432 ¥ 1080 ¥ 2160 ¥ 6480 ¥ 16200
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序列
用法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
儲存
避免反復(fù)凍融。2-8°C不超過一個(gè)月,-80°C不超過12個(gè)月。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定,具體方法如下:在37°C孵育48小時(shí),沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi),在適當(dāng)?shù)臈l件下存儲,損失率低于5%。
增值服務(wù)
相關(guān)產(chǎn)品
編號 | 適用物種:Rattus norvegicus (Rat,大鼠) | 應(yīng)用(僅供研究使用,不用于臨床診斷!) |
RPA037Ra01 | 纖連蛋白(FN)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
PAA037Ra01 | 纖連蛋白(FN)多克隆抗體 | WB; IHC; ICC; IP. |
FAA037Ra01 | 抗纖連蛋白(FN)多克隆抗體 | Flow cytometry. |
LAA037Ra81 | 纖連蛋白(FN)多克隆抗體(異硫氰酸熒光素標(biāo)記) | WB; IHC; ICC; IF. |
LAA037Ra71 | 纖連蛋白(FN)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
MAA037Ra21 | 纖連蛋白(FN)單克隆抗體 | WB; IHC; ICC; IP. |
FAA037Ra02 | 抗纖連蛋白(FN)單克隆抗體 | Flow cytometry. |
SEA037Ra | 纖連蛋白(FN)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
LMA037Ra | 纖連蛋白(FN)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
KSA037Ra01 | 纖連蛋白(FN)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻(xiàn)
雜志 | 參考文獻(xiàn) |
American Journal of Physiology - Renal Physiology | Advanced oxidation protein products induce mesangial cell perturbation through PKC-dependent activation of NADPH oxidase [PubMed: 19019916] |
Stem Cell Research Therapy | Protein synthesis and secretion in human mesenchymal cells derived from bone marrow, adipose tissue and Wharton's jelly [Pubmed: 24739658] |
Molecules. | Identification of compounds from the water soluble extract of Cinnamomum cassia barks and their inhibitory effects against high-glucose-induced mesangial cells. [Pubmed: 24013407] |
Journal of Ethnopharmacology | Bioactive compounds from Cornus officinalis fruits and their effects on diabetic nephropathy [ScienceDirect: S0378874114002414] |
Investigative Ophthalmology & Visual Science | Inhibition by Female Sex Hormones of Collagen Gel Contraction Mediated by Retinal Pigment Epithelial Cells [Pubmed: 24609629] |
PLoS One. | Mesenchymal stromal cell proliferation, gene expression and protein production in human platelet-rich plasma-supplemented media [Pubmed:Pmc4130592] |
Connective Tissue Research | Metabolic and cytoprotective effects of in vivo peri-patellar hyaluronic acid injections in cultured tenocytes [Pubmed:25333747] |
Laryngoscope. | Establishing principles of macromolecular crowding for in vitro fibrosis research of the vocal fold lamina propria [Pubmed:25545625] |
Biomedical Materials | In vivo bioengineered ovarian tumors based on collagen, matrigel, alginate and agarose hydrogels: a comparative study [Pubmed:25634132] |
Biomedical Materials | In vivo [PubMed: 25634132] |
American Journal of Medical and Biological Research | Impact of Nigella Sativa, Omega-3 Fatty Acids and Chromium Picolinate onNF-κB/leptin-insulin Axisin Obese Subjects with Non-alcoholic Fatty Liver Disease [Ajmbr: 3] |
Food Chem. | Comparative proteomics of milk fat globule membrane in goat colostrum and mature milk [Pubmed:27173528] |
journal of perinatal medicine | Maternal endothelial damage as a disorder shared by early preeclampsia, late preeclampsia and intrauterine growth restriction. [pubmed:27865093] |
Chinese Journal of Contemporary Neurology and Neurosurgery | Effect of panax notoginseng saponins on efficacy and hemorrhagic transformation of rt-PA intravenous thrombolysis in patients with acute ischemic stroke [Article_en:CJFDTotal-XDJB201611014] |
Scientific Reports | Serum fbronectin distinguishes the early stages of hepatocellular carcinoma [pubmed:28842594] |
Molecular Medicine Reports | Pigment epithelium?derived factor protects human glomerular mesangial cells from diabetes via NOXO1?iNOS suppression [10.3892/mmr.2017.7563] |
Cell Death & Disease | Tenomodulin is essential for prevention of adipocyte accumulation and fibrovascular scar formation during early tendon healing. [pubmed:29022912] |
Journal of?Cellular?Biochemistry | Effects of panax notoginseng saponin on the pathological ultrastructure and serum IL‐6 and IL‐8 in pulmonary fibrosis in rabbits [Pubmed:29932250] |
Biomedicine & Pharmacotherapy | Corn silk (Zea mays L.), a source of natural antioxidants with α-amylase, α-glucosidase, advanced glycation and diabetic nephropathy inhibitory activities [Pubmed: 30530231] |
Advanced?Healthcare?Materials | Impact of Hydrogel Elasticity and Adherence on Osteosarcoma Cells and Osteoblasts [Pubmed: 30838809] |
Inflammation | TNF-α Regulates ITGβ1 and SYND4 Expression in Nucleus Pulposus Cells: Activation of FAK/PI3K Signaling [Pubmed: 31111299] |
EBioMedicine | Three kinds of corneal host cells contribute differently to corneal neovascularization [] |
Renal Failure | Effects of autophagy on macrophage adhesion and migration in diabetic nephropathy [Pubmed: 31352855] |
Journal of Biological Chemistry | Growth hormone induces Notch1 signaling in podocytes and contributes to proteinuria in diabetic nephropathy [Pubmed: 31511328] |
Neurotherapeutics | Fabrication and Evaluation of a Xenogeneic Decellularized Nerve-Derived Material: Preclinical Studies of a New Strategy for Nerve Repair [Pubmed: 31758411] |
FASEB Journal | Molecular hydrogen regulates PTEN‐AKT‐mTOR signaling via ROS to alleviate peritoneal dialysis‐related peritoneal fibrosis [Pubmed: 31930571] |
Journal of Clinical Medicine | Damage-Associated Molecular Patterns and Th-Cell-Related Cytokines Released after Progressive Effort [Pubmed: 32210109] |
International Journal of Women?ˉs Health and Reproduction Sciences | Are the Circulating Levels of Copeptin and Fibronectin Dysregulated in Preeclamptic South African Black Women? [] |
Advanced glycation end-products associate with podocytopathy in type II diabetic patients [] | |
IBRO Neuroscience Reports | Early mobilization in spinal cord injury promotes changes in microglial dynamics and recovery of motor function [Pubmed:35586775] |
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