谷氨酰胺合成酶(GS)重組蛋白
Recombinant Glutamine synthetase (GS)
GLNS; GLUL; Glutamate-Ammonia Ligase; Glutamate decarboxylase
- 編號RPD761Ra01
- 物種Rattus norvegicus (Rat,大鼠) 相同的名稱,不同的物種。
- 來源原核表達
- 宿主E.coli
- 內(nèi)毒素水平<1.0EU/µg(LAL法測定)
- 亞細胞定位分泌
- 預測分子量43.5kDa
- 實際分子量44kDa(差異分析請參閱說明書)
- 片段與標簽Met1~Asn373 with N-terminal His Tag
- 緩沖液成份100mMNaHCO3, 500mMNaCl, pH8.3, containing 0.01% SKL, 5% Trehalose.
- 性狀凍干粉
- 純度> 95%
- 等電點6.8
-
應用
Positive Control; Immunogen; SDS-PAGE; WB.
如果需要有生物活性的蛋白,請參見活性蛋白。 - 下載 英文說明書 中文說明書
- 規(guī)格 10μg50μg 200μg 1mg 5mg
- 價格 ¥ 1008 ¥ 2520 ¥ 5040 ¥ 15120 ¥ 37800
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序列
用法
Reconstitute in ddH2O to a concentration of 0.1-1.0 mg/mL. Do not vortex.
儲存
避免反復凍融。2-8°C不超過一個月,-80°C不超過12個月。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定,具體方法如下:在37°C孵育48小時,沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi),在適當?shù)臈l件下存儲,損失率低于5%。
增值服務
相關產(chǎn)品
編號 | 適用物種:Rattus norvegicus (Rat,大鼠) | 應用(僅供研究使用,不用于臨床診斷!) |
RPD761Ra01 | 谷氨酰胺合成酶(GS)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
PAD761Ra01 | 谷氨酰胺合成酶(GS)多克隆抗體 | WB; IHC; ICC; IP. |
LAD761Ra71 | 谷氨酰胺合成酶(GS)多克隆抗體(生物素標記) | WB; IHC; ICC. |
LAD761Ra81 | 谷氨酰胺合成酶(GS)多克隆抗體(異硫氰酸熒光素標記) | WB; IHC; ICC; IF. |
MAD761Ra21 | 谷氨酰胺合成酶(GS)單克隆抗體 | WB; IHC; ICC; IP. |
SED761Ra | 谷氨酰胺合成酶(GS)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
LMD761Ra | 谷氨酰胺合成酶(GS)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻
雜志 | 參考文獻 |
The FASEB Journal | Cargo proteins of plasma astrocyte-derived exosomes in Alzheimer’s disease [pubmed:27511944] |
Annals of Neurology? | High complement levels in astrocyte‐derived exosomes of Alzheimer disease [Pubmed:29406582] |
Journal of Neuroscience | Sex differences in the glutamate signaling pathway in juvenile rats [Pubmed:28861894] |
FASEB?Journal | Deficient neurotrophic factors of CSPG4-type neural cell exosomes in Alzheimer disease [Pubmed:29924942] |
Hepatology Research | Glutamine synthetase promotes tumor invasion in hepatocellular carcinoma through mediating epithelial–mesenchymal transition [Pubmed: 31652385] |
FASEB JOURNAL | Traumatic brain injury increases plasma astrocyte‐derived exosome levels of neurotoxic complement proteins [Pubmed: 31916313] |
Translational Psychiatry | Decreased mitochondrial electron transport proteins and increased complement mediators in plasma neural-derived exosomes of early psychosis [Pubmed: 33106473] |
Drug Repurposing of Asparaginase and Vitamin C Targeting Glutamine Synthetase Improves Anticancer Effect in Metastatic Castration-resistant Prostate?… [] |
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