磷脂酰肌醇蛋白聚糖4(GPC4)重組蛋白
Recombinant Glypican 4 (GPC4)
K-Glypican; Glypican Proteoglycan 4; Secreted glypican-4
- 編號(hào)RPA998Mu01
- 物種Mus musculus (Mouse，小鼠) 相同的名稱，不同的物種。
- 來(lái)源原核表達(dá)
- 宿主E.coli
- 內(nèi)毒素水平<1.0EU/µg(LAL法測(cè)定)
- 亞細(xì)胞定位細(xì)胞膜, 細(xì)胞外基質(zhì)
- 預(yù)測(cè)分子量24.2kDa
- 實(shí)際分子量27kDa(差異分析請(qǐng)參閱說(shuō)明書(shū))
- 片段與標(biāo)簽Thr220~Gly426 with N-terminal His Tag
- 緩沖液成份20mM Tris, 150mM NaCl緩沖液（pH8.0, 含有1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose和Proclin300）
- 性狀凍干粉
- 純度> 97%
- 等電點(diǎn)6.5
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應(yīng)用
Positive Control; Immunogen; SDS-PAGE; WB.
如果需要有生物活性的蛋白，請(qǐng)參見(jiàn)活性蛋白。 - 下載 英文說(shuō)明書(shū) 中文說(shuō)明書(shū)
- 規(guī)格 10μg50μg 200μg 1mg 5mg
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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Figure . Gene Sequencing (extract)
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SDS-PAGE圖
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通過(guò)ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
序列
用法
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
儲(chǔ)存
避免反復(fù)凍融。2-8°C不超過(guò)一個(gè)月，-80°C不超過(guò)12個(gè)月。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定，具體方法如下：在37°C孵育48小時(shí)，沒(méi)有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲(chǔ)，損失率低于5%。
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相關(guān)產(chǎn)品
| 編號(hào) | 適用物種：Mus musculus (Mouse，小鼠) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| RPA998Mu02 | 磷脂酰肌醇蛋白聚糖4(GPC4)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA998Mu01 | 磷脂酰肌醇蛋白聚糖4(GPC4)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APA998Mu01 | 磷脂酰肌醇蛋白聚糖4(GPC4)活性蛋白 | Cell?culture;?Activity?Assays. |
| PAA998Mu02 | 磷脂酰肌醇蛋白聚糖4(GPC4)多克隆抗體 | WB; IHC; ICC; IP. |
| PAA998Mu01 | 磷脂酰肌醇蛋白聚糖4(GPC4)多克隆抗體 | WB; IHC; ICC; IP. |
| SEA998Mu | 磷脂酰肌醇蛋白聚糖4(GPC4)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA998Mu | 磷脂酰肌醇蛋白聚糖4(GPC4)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| PLoS One. | Identification of adipokine clusters related to parameters of fat mass, insulin sensitivity and inflammation [Pubmed:Pmc4072672] |
| Aspects of fluid therapy in the critically ill. Experimental and clinical studies on fluid therapy in the inflammatory conditions. [250690_2_G5_Svajunas_S.pdf] | |
| Intensive?Care?Medicine?Experimental | Elevated plasma glypicans are associated with organ failure in patients with infection [Pubmed: 30618011] |
| Crit Care | Albumin infusion rate and plasma volume expansion: a randomized clinical trial in postoperative patients after major surgery [Pubmed: 31138247] |
| J Endocrinol Invest | Serum glypican4 and glycosylphosphatidylinositol-specific phospholipase D levels are associated with adipose tissue insulin resistance in obese subjects with … [Pubmed: 32816247] |
| Heparin Binding Protein and Endothelial Glycocalyx Markers in Severe COVID-19–A Prospective Observational Cohort Study [] | |
| Sci Rep | Glypican-4 in pregnancy and its relation to glucose metabolism, insulin resistance and gestational diabetes mellitus status [34903856] |
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