Ⅰ型膠原α1(COL1a1)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法)
Multiplex Assay Kit for Collagen Type I Alpha 1 (COL1a1) ,etc. by FLIA (Flow Luminescence Immunoassay)
COL-1A1; COL1-A1; COL1A-1; OI4; Collagen Alpha-1(I)chain
（注：?jiǎn)未位鞙y(cè)多因子不超過(guò)8個(gè)指標(biāo) ）
- 編號(hào)LMA350Hu
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 實(shí)驗(yàn)方法雙抗夾心
- 反應(yīng)時(shí)長(zhǎng)3.5h
- 檢測(cè)范圍1.95-2000pg/mL
- 靈敏度最小可檢測(cè)劑量小于等于0.65 pg/mL.
- 樣本類型Serum, plasma and other biological fluids
- 下載 英文說(shuō)明書 中文說(shuō)明書
- 規(guī)格 8指標(biāo)數(shù) 7指標(biāo)數(shù) 6指標(biāo)數(shù) 5指標(biāo)數(shù) 4指標(biāo)數(shù) 3指標(biāo)數(shù) 2指標(biāo)數(shù)1指標(biāo)數(shù)
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通過(guò)ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
本試劑盒用于檢測(cè)Ⅰ型膠原α1(COL1a1)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法)，經(jīng)檢測(cè)與其它相似物質(zhì)無(wú)明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來(lái)源的限制，不可能完成對(duì)所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測(cè)，因此本試劑盒有可能與未經(jīng)檢測(cè)的其它物質(zhì)有交叉反應(yīng)。
回收率
分別于定值血清及血漿樣本中加入一定量的Ⅰ型膠原α1(COL1a1)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法)（加標(biāo)樣品），重復(fù)測(cè)定并計(jì)算其均值，回收率為測(cè)定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 86-101 | 94 |
| EDTA plasma(n=5) | 96-105 | 101 |
| heparin plasma(n=5) | 93-101 | 96 |
| sodium citrate plasma(n=5) | 90-97 | 93 |
精密度
精密度用樣品測(cè)定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對(duì)低、中、高值定值樣本進(jìn)行定量檢測(cè),每份樣本連續(xù)測(cè)定20 次，分別計(jì)算不同濃度樣本的平均值及SD值。
批間差：選取3個(gè)不同批次的試劑盒分別對(duì)低、中、高值定值樣本進(jìn)行定量測(cè)定，每個(gè)樣本使用同一試劑盒重復(fù)測(cè)定8次，分別計(jì)算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的Ⅰ型膠原α1(COL1a1)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法)，并倍比稀釋成1:2，1:4，1:8，1:16的待測(cè)樣本，線性范圍即為稀釋后樣本中Ⅰ型膠原α1(COL1a1)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法)含量的測(cè)定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 78-94% | 80-95% | 82-90% | 91-98% |
| EDTA plasma(n=5) | 90-97% | 81-104% | 93-105% | 78-98% |
| heparin plasma(n=5) | 92-101% | 93-102% | 95-105% | 85-97% |
| sodium citrate plasma(n=5) | 81-103% | 86-94% | 87-95% | 97-105% |
穩(wěn)定性
經(jīng)測(cè)定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對(duì)試劑盒破壞前后檢測(cè)值的影響，實(shí)驗(yàn)室的環(huán)境條件需盡量保持一致，尤其是實(shí)驗(yàn)室內(nèi)溫度、濕度及溫育條件。其次由同一實(shí)驗(yàn)員來(lái)進(jìn)行操作可減少人為誤差。
實(shí)驗(yàn)流程
1. 實(shí)驗(yàn)前標(biāo)準(zhǔn)品、試劑及樣本準(zhǔn)備；
2. 加樣（標(biāo)準(zhǔn)品、樣本、磁珠）標(biāo)準(zhǔn)品或樣本100μL及磁珠10μL，
37°C酶標(biāo)板振蕩器孵育90分鐘；
3. 磁吸甩干，加檢測(cè)溶液A100μL，37°C酶標(biāo)板振蕩器孵育60分鐘；
4. 磁吸洗板3次；
5. 加檢測(cè)溶液B100μL，37°C振動(dòng)孵育30分鐘；
6. 磁吸洗板3次；
7. 加鞘液100μL，旋渦震蕩2分鐘后讀數(shù)。
實(shí)驗(yàn)原理
將Ⅰ型膠原α1(COL1a1)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法)抗體包被于磁珠，制成固相載體，向微孔中分別加入標(biāo)準(zhǔn)品或標(biāo)本以及磁珠，其中的Ⅰ型膠原α1(COL1a1)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法)與連接于固相載體上的抗體結(jié)合，然后加入生物素化的Ⅰ型膠原α1(COL1a1)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法)抗體，將未結(jié)合的生物素化抗體洗凈后，加入PE標(biāo)記的親和素，再次徹底洗滌后即可上機(jī)讀數(shù)。MFI值和樣品中的Ⅰ型膠原α1(COL1a1)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法)呈正相關(guān)。
贈(zèng)品
相關(guān)產(chǎn)品
| 編號(hào) | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| EPA350Hu61 | Ⅰ型膠原α1(COL1a1)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA350Hu04 | Ⅰ型膠原α1(COL1a1)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| EPA350Hu62 | Ⅰ型膠原α1(COL1a1)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA350Hu03 | Ⅰ型膠原α1(COL1a1)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA350Hu01 | Ⅰ型膠原α1(COL1a1)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APA350Hu61 | Ⅰ型膠原α1(COL1a1)活性蛋白 | Cell?culture;?Activity?Assays. |
| RPA350Hu02 | Ⅰ型膠原α1(COL1a1)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| CPA350Hu11 | Ⅰ型膠原α1(COL1a1)牛血清白蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
| CPA350Hu21 | Ⅰ型膠原α1(COL1a1)卵白蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
| PAA350Hu01 | Ⅰ型膠原α1(COL1a1)多克隆抗體 | WB; IHC; ICC; IP. |
| PAA350Hu03 | Ⅰ型膠原α1(COL1a1)多克隆抗體 | WB; IHC; ICC; IP. |
| PAA350Hu04 | Ⅰ型膠原α1(COL1a1)多克隆抗體 | WB; IHC; ICC; IP. |
| PAA350Hu08 | Ⅰ型膠原α1(COL1a1)多克隆抗體 | WB; IHC; ICC; IP. |
| PAA350Hu02 | Ⅰ型膠原α1(COL1a1)多克隆抗體 | WB |
| MAA350Hu22 | Ⅰ型膠原α1(COL1a1)單克隆抗體 | WB |
| MAA350Hu21 | Ⅰ型膠原α1(COL1a1)單克隆抗體 | WB; IHC |
| MAA350Hu23 | Ⅰ型膠原α1(COL1a1)單克隆抗體 | WB; IHC; ICC; IP. |
| FAA350Hu02 | 抗Ⅰ型膠原α1(COL1a1)單克隆抗體 | Flow cytometry. |
| SEA350Hu | Ⅰ型膠原α1(COL1a1)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA350Hu | Ⅰ型膠原α1(COL1a1)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology and Endodontology | Simvastatin-loaded porous implant surfaces stimulate preosteoblasts differentiation: an in vitro study [ScienceDirect: S1079210410004919] |
| Journal of Biomedical Materials | Osteoblast response to puerarin-loaded porous titanium surfaces: An in vitro study [Wiley: source] |
| European Archives of Oto-Rhino-Laryngology | Age effects on extracellular matrix production of vocal fold scar fibroblasts in rats [Pubmed: 24077847] |
| J Voice. | Vocal Fold Fibroblast Response to Growth Factor Treatment is Age Dependent: Results From an In Vitro Study [Pubmed: 24495429] |
| Arch Oral Biol. | Porphyromonas gingivalis LPS inhibits osteoblastic differentiation and promotes pro-inflammatory cytokine production in human periodontal ligament stem cells. [Pubmed: 24370188] |
| Laryngoscope. | Establishing principles of macromolecular crowding for in vitro fibrosis research of the vocal fold lamina propria [Pubmed:25545625] |
| UNIVERSA MEDICINA | Allogeneic human dermal fibroblasts are viable in peripheral blood mononuclear co-culture [Univmed:Source] |
| International Journal of?Neuropsychopharmacology | The vitamin D receptor agonist, calcipotriol, modulates fibrogenic pathways mitigating liver fibrosis in-vivo: An experimental study. [pubmed:27477355] |
| National Natural Scientific | Role of Gut-Derived Endotoxin on Type I Collagen Production in the Rat Pancreas after Chronic Alcohol Exposure [pubmed:29121396] |
| Nephron. | Prevention of Diabetic Nephropathy by Modified Acidic Fibroblast Growth Factor [pubmed:28768285] |
| Cell?and?Tissue?Research | Effect of TGFβ1, TGFβ3 and keratinocyte conditioned media on functional characteristics of dermal fibroblasts derived from reparative (Balb/c) and?… [Pubmed:29637306] |
| International Journal of Molecular?Medicine | LOXL2, a copper-dependent monoamine oxidase, activates lung fibroblasts through the TGF-β/Smad pathway [Pubmed: 30320382] |
| International Journal of Molecular?Sciences | Association between Plasma HMGB-1 and Silicosis: A Case-Control Study [Pubmed: 30558126] |
| Journal of Proteomics | Salivary proteins from dysplastic leukoplakia and oral squamous cell carcinoma and their potential for early detection [Pubmed: 31706945] |
| Brazilian Journal of Pharmaceutical Sciences | Hesperidin inhibits the epithelial to mesenchymal transition induced by transforming growth factor-β1 in A549 cells through Smad signaling in the cytoplasm [] |
| Ceramics International | Platinum nanoparticles supported on functionalized hydroxyapatite: Anti-oxidant properties and bone cells response [] |
| Scientific reports | Inhibition of eEF2K synergizes with glutaminase inhibitors or 4EBP1 depletion to suppress growth of triple-negative breast cancer cells [33911160] |
| Int J Mol Sci | Antiosteoporotic Nanohydroxyapatite Zoledronate Scaffold Seeded with Bone Marrow Mesenchymal Stromal Cells for Bone Regeneration: A 3D In Vitro Model [Pubmed:35682677] |
