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Ⅰ型膠原α1(COL1a1)活性蛋白

Active Collagen Type I Alpha 1 (COL1a1)

COL-1A1; COL1-A1; COL1A-1; OI4; Collagen Alpha-1(I)chain

  • Ⅰ型膠原α1(COL1a1)活性蛋白 產品包裝(模擬)
  • Ⅰ型膠原α1(COL1a1)活性蛋白 產品包裝(模擬)
  • APA350Hu61.jpg SDS-PAGE圖
  • Certificate 通過ISO 9001、ISO 13485質量體系認證

活性實驗

Type I collagen is a fibril-forming collagen found in most connective tissues and is abundant in bone, cornea, dermis and tendon. It is a heterotrimer that consists of two alpha 1 chains and one alpha 2 chain, although homotrimers consisting of three identical alpha 1 chains have also been described. Thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human COL1a1 and recombinant human COL1a2. Briefly, biotin-linked COL1a1 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to COL1a2-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST 3 times and incubation with Streptavidin-HRP for 30min, then wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 μl stop solution to the wells and read at 450 nm immediately. The binding activity of COL1a1 and COL1a2 was shown in Figure 1, the EC50?for this effect is 10.13 ug/mL.

用法

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

儲存

避免反復凍融。2-8°C不超過一個月，-80°C不超過12個月。

穩(wěn)定性

熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產生。保質期內，在適當?shù)臈l件下存儲，損失率低于5%。

相關產品

編號 適用物種：Homo sapiens (Human，人) 應用(僅供研究使用，不用于臨床診斷！)
EPA350Hu61 Ⅰ型膠原α1(COL1a1)真核蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPA350Hu04 Ⅰ型膠原α1(COL1a1)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
EPA350Hu62 Ⅰ型膠原α1(COL1a1)真核蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPA350Hu03 Ⅰ型膠原α1(COL1a1)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPA350Hu01 Ⅰ型膠原α1(COL1a1)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APA350Hu61 Ⅰ型膠原α1(COL1a1)活性蛋白 Cell?culture;?Activity?Assays.
RPA350Hu02 Ⅰ型膠原α1(COL1a1)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
CPA350Hu11 Ⅰ型膠原α1(COL1a1)牛血清白蛋白偶聯(lián)物 Immunogen; SDS-PAGE; WB.
CPA350Hu21 Ⅰ型膠原α1(COL1a1)卵白蛋白偶聯(lián)物 Immunogen; SDS-PAGE; WB.
PAA350Hu01 Ⅰ型膠原α1(COL1a1)多克隆抗體 WB; IHC; ICC; IP.
PAA350Hu03 Ⅰ型膠原α1(COL1a1)多克隆抗體 WB; IHC; ICC; IP.
PAA350Hu04 Ⅰ型膠原α1(COL1a1)多克隆抗體 WB; IHC; ICC; IP.
PAA350Hu08 Ⅰ型膠原α1(COL1a1)多克隆抗體 WB; IHC; ICC; IP.
PAA350Hu02 Ⅰ型膠原α1(COL1a1)多克隆抗體 WB
MAA350Hu22 Ⅰ型膠原α1(COL1a1)單克隆抗體 WB
MAA350Hu21 Ⅰ型膠原α1(COL1a1)單克隆抗體 WB; IHC
MAA350Hu23 Ⅰ型膠原α1(COL1a1)單克隆抗體 WB; IHC; ICC; IP.
FAA350Hu02 抗Ⅰ型膠原α1(COL1a1)單克隆抗體 Flow cytometry.
SEA350Hu Ⅰ型膠原α1(COL1a1)檢測試劑盒(酶聯(lián)免疫吸附試驗法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA350Hu Ⅰ型膠原α1(COL1a1)等多因子檢測試劑盒(流式熒光發(fā)光法) FLIA Kit for Antigen Detection.

參考文獻

雜志 參考文獻
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Journal of Biomedical Materials Osteoblast response to puerarin-loaded porous titanium surfaces: An in vitro study [Wiley: source]
European Archives of Oto-Rhino-Laryngology Age effects on extracellular matrix production of vocal fold scar fibroblasts in rats [Pubmed: 24077847]
J Voice. Vocal Fold Fibroblast Response to Growth Factor Treatment is Age Dependent: Results From an In Vitro Study [Pubmed: 24495429]
Arch Oral Biol. Porphyromonas gingivalis LPS inhibits osteoblastic differentiation and promotes pro-inflammatory cytokine production in human periodontal ligament stem cells. [Pubmed: 24370188]
Laryngoscope. Establishing principles of macromolecular crowding for in vitro fibrosis research of the vocal fold lamina propria [Pubmed:25545625]
UNIVERSA MEDICINA Allogeneic human dermal fibroblasts are viable in peripheral blood mononuclear co-culture [Univmed:Source]
International Journal of?Neuropsychopharmacology The vitamin D receptor agonist, calcipotriol, modulates fibrogenic pathways mitigating liver fibrosis in-vivo: An experimental study. [pubmed:27477355]
National Natural Scientific Role of Gut-Derived Endotoxin on Type I Collagen Production in the Rat Pancreas after Chronic Alcohol Exposure [pubmed:29121396]
Nephron. Prevention of Diabetic Nephropathy by Modified Acidic Fibroblast Growth Factor [pubmed:28768285]
Cell?and?Tissue?Research Effect of TGFβ1, TGFβ3 and keratinocyte conditioned media on functional characteristics of dermal fibroblasts derived from reparative (Balb/c) and?… [Pubmed:29637306]
International Journal of Molecular?Medicine LOXL2, a copper-dependent monoamine oxidase, activates lung fibroblasts through the TGF-β/Smad pathway [Pubmed: 30320382]
International Journal of Molecular?Sciences Association between Plasma HMGB-1 and Silicosis: A Case-Control Study [Pubmed: 30558126]
Journal of Proteomics Salivary proteins from dysplastic leukoplakia and oral squamous cell carcinoma and their potential for early detection [Pubmed: 31706945]
Brazilian Journal of Pharmaceutical Sciences Hesperidin inhibits the epithelial to mesenchymal transition induced by transforming growth factor-β1 in A549 cells through Smad signaling in the cytoplasm []
Ceramics International Platinum nanoparticles supported on functionalized hydroxyapatite: Anti-oxidant properties and bone cells response []
Scientific reports Inhibition of eEF2K synergizes with glutaminase inhibitors or 4EBP1 depletion to suppress growth of triple-negative breast cancer cells [33911160]
Int J Mol Sci Antiosteoporotic Nanohydroxyapatite Zoledronate Scaffold Seeded with Bone Marrow Mesenchymal Stromal Cells for Bone Regeneration: A 3D In Vitro Model [Pubmed:35682677]
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