Toll樣受體2(TLR2)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法)
Multiplex Assay Kit for Toll Like Receptor 2 (TLR2) ,etc. by FLIA (Flow Luminescence Immunoassay)
CD282; TIL4; Toll/interleukin-1 receptor-like protein 4
（注：單次混測(cè)多因子不超過8個(gè)指標(biāo) ）
- 編號(hào)LMA663Hu
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 實(shí)驗(yàn)方法雙抗夾心
- 反應(yīng)時(shí)長3.5h
- 檢測(cè)范圍0.02-20ng/mL
- 靈敏度最小可檢測(cè)劑量小于等于0.007 ng/mL.
- 樣本類型Saliva, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下載 英文說明書 中文說明書
- 規(guī)格 8指標(biāo)數(shù) 7指標(biāo)數(shù) 6指標(biāo)數(shù) 5指標(biāo)數(shù) 4指標(biāo)數(shù) 3指標(biāo)數(shù) 2指標(biāo)數(shù)1指標(biāo)數(shù)
- 價(jià)格 ¥ 2090 ¥ 2171 ¥ 2291 ¥ 2452 ¥ 2613 ¥ 2854 ¥ 3216 ¥ 4020 添加到價(jià)格計(jì)算器 計(jì)算器
- 欲了解實(shí)際交易價(jià)格和更多情況，請(qǐng)與當(dāng)?shù)亟?jīng)銷商聯(lián)系!
-
產(chǎn)品包裝(模擬)
-
產(chǎn)品包裝(模擬)
-
通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
本試劑盒用于檢測(cè)Toll樣受體2(TLR2)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法)，經(jīng)檢測(cè)與其它相似物質(zhì)無明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來源的限制，不可能完成對(duì)所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測(cè)，因此本試劑盒有可能與未經(jīng)檢測(cè)的其它物質(zhì)有交叉反應(yīng)。
回收率
分別于定值血清及血漿樣本中加入一定量的Toll樣受體2(TLR2)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法)（加標(biāo)樣品），重復(fù)測(cè)定并計(jì)算其均值，回收率為測(cè)定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 94-102 | 97 |
| EDTA plasma(n=5) | 82-101 | 96 |
| heparin plasma(n=5) | 92-101 | 96 |
| sodium citrate plasma(n=5) | 91-102 | 95 |
精密度
精密度用樣品測(cè)定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對(duì)低、中、高值定值樣本進(jìn)行定量檢測(cè),每份樣本連續(xù)測(cè)定20 次，分別計(jì)算不同濃度樣本的平均值及SD值。
批間差：選取3個(gè)不同批次的試劑盒分別對(duì)低、中、高值定值樣本進(jìn)行定量測(cè)定，每個(gè)樣本使用同一試劑盒重復(fù)測(cè)定8次，分別計(jì)算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的Toll樣受體2(TLR2)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法)，并倍比稀釋成1:2，1:4，1:8，1:16的待測(cè)樣本，線性范圍即為稀釋后樣本中Toll樣受體2(TLR2)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法)含量的測(cè)定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 78-89% | 96-103% | 83-101% | 81-104% |
| EDTA plasma(n=5) | 97-104% | 96-103% | 91-98% | 80-99% |
| heparin plasma(n=5) | 78-91% | 86-103% | 82-97% | 78-94% |
| sodium citrate plasma(n=5) | 80-104% | 94-105% | 93-101% | 94-101% |
穩(wěn)定性
經(jīng)測(cè)定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對(duì)試劑盒破壞前后檢測(cè)值的影響，實(shí)驗(yàn)室的環(huán)境條件需盡量保持一致，尤其是實(shí)驗(yàn)室內(nèi)溫度、濕度及溫育條件。其次由同一實(shí)驗(yàn)員來進(jìn)行操作可減少人為誤差。
實(shí)驗(yàn)流程
1. 實(shí)驗(yàn)前標(biāo)準(zhǔn)品、試劑及樣本準(zhǔn)備；
2. 加樣（標(biāo)準(zhǔn)品、樣本、磁珠）標(biāo)準(zhǔn)品或樣本100μL及磁珠10μL，
37°C酶標(biāo)板振蕩器孵育90分鐘；
3. 磁吸甩干，加檢測(cè)溶液A100μL，37°C酶標(biāo)板振蕩器孵育60分鐘；
4. 磁吸洗板3次；
5. 加檢測(cè)溶液B100μL，37°C振動(dòng)孵育30分鐘；
6. 磁吸洗板3次；
7. 加鞘液100μL，旋渦震蕩2分鐘后讀數(shù)。
實(shí)驗(yàn)原理
將Toll樣受體2(TLR2)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法)抗體包被于磁珠，制成固相載體，向微孔中分別加入標(biāo)準(zhǔn)品或標(biāo)本以及磁珠，其中的Toll樣受體2(TLR2)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法)與連接于固相載體上的抗體結(jié)合，然后加入生物素化的Toll樣受體2(TLR2)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法)抗體，將未結(jié)合的生物素化抗體洗凈后，加入PE標(biāo)記的親和素，再次徹底洗滌后即可上機(jī)讀數(shù)。MFI值和樣品中的Toll樣受體2(TLR2)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法)呈正相關(guān)。
贈(zèng)品
相關(guān)產(chǎn)品
| 編號(hào) | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| APA663Hu04 | Toll樣受體2(TLR2)活性蛋白 | Cell?culture;?Activity?Assays. |
| APA663Hu02 | Toll樣受體2(TLR2)活性蛋白 | Cell?culture;?Activity?Assays. |
| RPA663Hu02 | Toll樣受體2(TLR2)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA663Hu01 | Toll樣受體2(TLR2)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA663Hu03 | Toll樣受體2(TLR2)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA663Hu04 | Toll樣受體2(TLR2)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA663Hu01 | Toll樣受體2(TLR2)多克隆抗體 | WB; IHC; ICC; IP. |
| PAA663Hu02 | Toll樣受體2(TLR2)多克隆抗體 | WB; IHC; ICC; IP. |
| PAA663Hu04 | Toll樣受體2(TLR2)多克隆抗體 | WB; IHC; ICC; IP. |
| PAA663Hu03 | Toll樣受體2(TLR2)多克隆抗體 | WB; IHC; ICC; IP. |
| LAA663Hu81 | Toll樣受體2(TLR2)多克隆抗體(異硫氰酸熒光素標(biāo)記) | WB; IHC; ICC; IF. |
| LAA663Hu71 | Toll樣受體2(TLR2)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| MAA663Hu25 | Toll樣受體2(TLR2)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA663Hu22 | Toll樣受體2(TLR2)單克隆抗體 | WB; IHC; ICC; IP. |
| RAA663Hu21 | Toll樣受體2(TLR2)重組抗體 | WB; IF; ICC; IHC; IP; FCM. |
| MAA663Hu21 | Toll樣受體2(TLR2)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA663Hu24 | Toll樣受體2(TLR2)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA663Hu23 | Toll樣受體2(TLR2)單克隆抗體 | WB; IHC; ICC; IP. |
| FAA663Hu02 | 抗Toll樣受體2(TLR2)單克隆抗體 | Flow cytometry. |
| FAA663Hu04 | 抗Toll樣受體2(TLR2)單克隆抗體 | FCM |
| LAA663Hu72 | Toll樣受體2(TLR2)單克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| SEA663Hu | Toll樣受體2(TLR2)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| MEA663Hu | Toll樣受體2(TLR2)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,小樣本) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA663Hu | Toll樣受體2(TLR2)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| PSA663Hu01 | Toll樣受體2(TLR2)抗體對(duì) | ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays. |
| KSA663Hu01 | Toll樣受體2(TLR2)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| Chinese Medical Journal | Expression of soluble Toll-like receptors in pleural effusions [CMJ: 2010823483999506297] |
| PLoS One | Baicalein Reduces Airway Injury in Allergen and IL-13 Induced Airway Inflammation [PubMed: PMC3639905] |
| Clinical and Developmental Immunology | TLR2 in Pleural Fluid Is Modulated by Talc Particles during Pleurodesis [PubMed: PMC3529880] |
| Advances in Virology | The Possible Role of TLR2 in Chronic Hepatitis B Patients with Precore Mutation [Pubmed: 24187552] |
| J Matern Fetal Neonatal Med. | Cervical and vaginal fluid soluble Toll-like receptor 2 in pregnancies complicated by preterm prelabor rupture of membranes [Pubmed:25028801] |
| Respiratory Virology and Immunogenicity Advances in Experimental Medicine and Biology | Cytokines and Toll-Like Receptors in the Immune Response to Influenza Vaccination [Pubmed:25248345] |
| Iran J Immunol. | Correlation?between?salivary?Toll like receptor-2?concentration?and early childhood caries. [Pubmed:25265998] |
| Int J Dermatol. | Membrane and soluble Toll‐like receptor 2 in patients with psoriasis treated by Goeckerman therapy [Pubmed:25266302] |
| BMC Immunology | The discriminative capacity of soluble Toll-like receptor (sTLR)2 and sTLR4 in inflammatory diseases [Pubmed:25406630] |
| Pathol Biol (Paris) | Cathelicidin related antimicrobial peptide, laminin, Toll-like receptors and chemokines levels in experimental hypersensitivity pneumonitis in mice [PubMed: 25834936] |
| Plos One | Soluble?Toll-Like Receptors?2?and?4?in Cerebrospinal Fluid?of?Patients?with?Acute?Hydrocephalus?followingAneurysmal Subarachnoid Haemorrhage. [Pubmed:27223696] |
| Open Forum Infect Dis | The Effect of Rosuvastatin on Markers of Immune Activation in Treatment-Naive Human Immunodeficiency Virus-Patients [Pubmed:26835476] |
| Perfusion | Pentraxin 3 and other inflammatory biomarkers related to atrial fibrillation in cardiac surgery [pubmed:27852958] |
| Journal For?Neurovirology | Soluble?TLR2?and?4?concentrations?in?cerebrospinal?fluid?in?HIV/SIV-relatedneuropathological?conditions. [pubmed:27882497] |
| Acta?Orthopaedica | Toll-like receptors and their soluble forms differ in the knee and thumb basal osteoarthritic joints [pubmed:28093922] |
| Biological Markers and Guided Therapy | Features of the Pattern Recognition Receptor TLR-2 Expression and Cytokines IL-2, IL-4 Profile in Children with Food Hypersensitivity [10.12988:bmgt.2018.812] |
| CHILD`S HEALTH | Prospective study on the significance of mucosal barrier dysfunction markers in assessing the effectiveness of the new strategy for treating food hypersensitivity in?… [10.22141:2224-0551.13.2.2018.129548] |
| Neurological Sciences | Vinpocetine regulates levels of circulating TLRs in Parkinson's disease patients [Pubmed: 30315378] |
| Biological Markers in Fundamental and Clinical Medicine | PROGNOSTIC ROLE OF IL-4, IL-2 AND TLR-2 FOR PREDICTING OF COURSE OF FOOD ALLERGY IN CHILDREN [Doi: 10.29256/v.02.02.2018.escbm71] |
| Biological Markers in Fundamental and Clinical Medicine | STUDY OF THE ACTION OF CHROMIUM-CONTAINING BIOPREPARATIONS FROM SPIRULINA ON INSULIN ACTIVITY IN RATS [Doi: 10.29256/v.02.02.2018.escbm72] |
| Biomedicine?& Pharmacotherapy | Opuntioside, opuntiol and its metallic nanoparticles attenuate adjuvant-induced arthritis: Novel suppressors of Toll-like receptors-2 and-4 [] |
| PLoS One | Aberrant DNA methylation of the toll-like receptors 2 and 6 genes in patients with obstructive sleep apnea [Pubmed: 32069296] |
| Georgian medical news | ASSOCIATIONS BETWEEN EFFICACY OF THE THERAPY AND CIRCADIAN FLUCTUATIONS OF ENDOTHELIAL NITRIC OXIDE SYNTHASE WITH TOLL-LIKE?… [Pubmed: 32242853] |
| Medical and Clinical Chemistry | ДОбОвА вАР?АбЕЛЬН?СТЬ Р?вНЯ РОзЧИННИХ ТОЛ-пОД?бНИХ РЕцЕпТОР?в 2 в СИРОвАТц? КРОв? Ж?НОК, ХвОРИХ НА РЕвМАТО?ДНИй АРТРИТ: зв' … [] |
| Sciences of Europe | FEATURES OF MYOCARDIAL REMODELING IN MEN WITH POST-INFARCTION CARDIOSCLEROSIS AND ITS RELATIONSHIP WITH TOLL-LIKE … [] |
| CLINICAL INVESTIGATIONS | Levels of toll-like receptors 2 and 4 in serum of men with postinfarction cardiosclerosis and its relationship with antiphospholipid syndrome components [] |
| Acta Obstet Gynecol Scand | A value of soluble Toll‐like receptor 2 and 4 in vaginally obtained amniotic fluid for the prediction of histological chorioamnionitis [34244993] |
| Clinical and Translational Allergy | Altered innate immune profile in blood of systemic mastocytosis patients [Pubmed:35734269] |
