Toll樣受體2(TLR2)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,小樣本)
Mini Samples ELISA Kit for Toll Like Receptor 2 (TLR2)
CD282; TIL4; Toll/interleukin-1 receptor-like protein 4
- 編號MEA663Hu
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 實(shí)驗(yàn)方法雙抗夾心
- 反應(yīng)時(shí)長3h
- 檢測范圍0.312-20ng/mL
- 靈敏度最小可檢測劑量小于等于0.121ng/mL.
- 樣本類型saliva,tissue homogenates, cell lysates,cell culture supernates and other biological fluids
- 下載 英文說明書 中文說明書
- 規(guī)格 48T96T 96T*5 96T*10 96T*100
- 價(jià)格 ¥ 2512 ¥ 3588 ¥ 16146 ¥ 30498 ¥ 251160
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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實(shí)驗(yàn)結(jié)果圖
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標(biāo)準(zhǔn)曲線圖
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通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
本試劑盒用于檢測Toll樣受體2(TLR2)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,小樣本)，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來源的限制，不可能完成對所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應(yīng)。
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進(jìn)行定量檢測,每份樣本連續(xù)測定20 次，分別計(jì)算不同濃度樣本的平均值及SD值。
批間差：選取3個(gè)不同批次的試劑盒分別對低、中、高值定值樣本進(jìn)行定量測定，每個(gè)樣本使用同一試劑盒重復(fù)測定8次，分別計(jì)算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
穩(wěn)定性
經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實(shí)驗(yàn)室的環(huán)境條件需盡量保持一致，尤其是實(shí)驗(yàn)室內(nèi)溫度、濕度及溫育條件。其次由同一實(shí)驗(yàn)員來進(jìn)行操作可減少人為誤差。
實(shí)驗(yàn)流程
1. 實(shí)驗(yàn)前標(biāo)準(zhǔn)品、試劑及樣本的準(zhǔn)備；
2. 加樣（標(biāo)準(zhǔn)品及樣本）25µL，37°C溫育1小時(shí)；
3. 吸棄，加檢測溶液A25µL，37°C孵育1小時(shí)；
4. 洗板3次；
5. 加檢測溶液B25µL，37°C孵育30分鐘；
6. 洗板5次；
7. 加TMB底物25µL，37°C孵育10-20分鐘；
8. 加終止液20µL，立即450nm讀數(shù)。
實(shí)驗(yàn)原理
將Toll樣受體2(TLR2)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,小樣本)抗體包被于96孔微孔板中，制成固相載體，向微孔中分別加入標(biāo)準(zhǔn)品或標(biāo)本，其中的Toll樣受體2(TLR2)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,小樣本)與連接于固相載體上的抗體結(jié)合，然后加入生物素化的Toll樣受體2(TLR2)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,小樣本)抗體，將未結(jié)合的生物素化抗體洗凈后，加入HRP標(biāo)記的親和素，再次徹底洗滌后加入TMB底物顯色。TMB在過氧化物酶的催化下轉(zhuǎn)化成藍(lán)色，并在酸的作用下轉(zhuǎn)化成最終的黃色。顏色的深淺和樣品中的Toll樣受體2(TLR2)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,小樣本)呈正相關(guān)。用酶標(biāo)儀在450nm波長下測定吸光度(O.D.值)，計(jì)算樣品濃度。
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相關(guān)產(chǎn)品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| APA663Hu04 | Toll樣受體2(TLR2)活性蛋白 | Cell?culture;?Activity?Assays. |
| APA663Hu02 | Toll樣受體2(TLR2)活性蛋白 | Cell?culture;?Activity?Assays. |
| RPA663Hu02 | Toll樣受體2(TLR2)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA663Hu01 | Toll樣受體2(TLR2)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA663Hu03 | Toll樣受體2(TLR2)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA663Hu04 | Toll樣受體2(TLR2)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA663Hu01 | Toll樣受體2(TLR2)多克隆抗體 | WB; IHC; ICC; IP. |
| PAA663Hu02 | Toll樣受體2(TLR2)多克隆抗體 | WB; IHC; ICC; IP. |
| PAA663Hu04 | Toll樣受體2(TLR2)多克隆抗體 | WB; IHC; ICC; IP. |
| PAA663Hu03 | Toll樣受體2(TLR2)多克隆抗體 | WB; IHC; ICC; IP. |
| LAA663Hu81 | Toll樣受體2(TLR2)多克隆抗體(異硫氰酸熒光素標(biāo)記) | WB; IHC; ICC; IF. |
| LAA663Hu71 | Toll樣受體2(TLR2)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| MAA663Hu25 | Toll樣受體2(TLR2)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA663Hu22 | Toll樣受體2(TLR2)單克隆抗體 | WB; IHC; ICC; IP. |
| RAA663Hu21 | Toll樣受體2(TLR2)重組抗體 | WB; IF; ICC; IHC; IP; FCM. |
| MAA663Hu21 | Toll樣受體2(TLR2)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA663Hu24 | Toll樣受體2(TLR2)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA663Hu23 | Toll樣受體2(TLR2)單克隆抗體 | WB; IHC; ICC; IP. |
| FAA663Hu02 | 抗Toll樣受體2(TLR2)單克隆抗體 | Flow cytometry. |
| FAA663Hu04 | 抗Toll樣受體2(TLR2)單克隆抗體 | FCM |
| LAA663Hu72 | Toll樣受體2(TLR2)單克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| SEA663Hu | Toll樣受體2(TLR2)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| MEA663Hu | Toll樣受體2(TLR2)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,小樣本) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA663Hu | Toll樣受體2(TLR2)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| PSA663Hu01 | Toll樣受體2(TLR2)抗體對 | ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays. |
| KSA663Hu01 | Toll樣受體2(TLR2)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) | Main materials for "Do It (ELISA Kit) Yourself". |
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