基質(zhì)金屬蛋白酶8(MMP8)重組蛋白
Recombinant Matrix Metalloproteinase 8 (MMP8)
CLG2; HNC; PMNL-CL; Collagenase 2; Neutrophil Collagenase; PMNL collagenase
- 編號RPA103Ra01
- 物種Rattus norvegicus (Rat,大鼠) 相同的名稱,不同的物種。
- 來源原核表達
- 宿主E.coli
- 內(nèi)毒素水平<1.0EU/µg(LAL法測定)
- 亞細胞定位分泌, 細胞外基質(zhì)
- 預(yù)測分子量31.3kDa
- 實際分子量38kDa(差異分析請參閱說明書)
- 片段與標(biāo)簽Leu211~Ser449 with N-terminal His Tag
- 緩沖液成份20mM Tris, 150mM NaCl緩沖液(pH8.0, 含有1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose和Proclin300)
- 性狀凍干粉
- 純度> 90%
- 等電點6.9
-
應(yīng)用
Positive Control; Immunogen; SDS-PAGE; WB.
如果需要有生物活性的蛋白,請參見活性蛋白。 - 下載 英文說明書 中文說明書
- 規(guī)格 10μg50μg 200μg 1mg 5mg
- 價格 ¥ 1080 ¥ 2700 ¥ 5400 ¥ 16200 ¥ 40500
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序列
用法
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
儲存
避免反復(fù)凍融。2-8°C不超過一個月,-80°C不超過12個月。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定,具體方法如下:在37°C孵育48小時,沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi),在適當(dāng)?shù)臈l件下存儲,損失率低于5%。
增值服務(wù)
相關(guān)產(chǎn)品
編號 | 適用物種:Rattus norvegicus (Rat,大鼠) | 應(yīng)用(僅供研究使用,不用于臨床診斷!) |
RPA103Ra01 | 基質(zhì)金屬蛋白酶8(MMP8)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
RPA103Ra02 | 基質(zhì)金屬蛋白酶8(MMP8)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
PAA103Ra01 | 基質(zhì)金屬蛋白酶8(MMP8)多克隆抗體 | WB; IHC; ICC; IP. |
MAA103Ra22 | 基質(zhì)金屬蛋白酶8(MMP8)單克隆抗體 | WB; IHC; ICC; IP. |
MAA103Ra21 | 基質(zhì)金屬蛋白酶8(MMP8)單克隆抗體 | WB; IHC; ICC; IP. |
SEA103Ra | 基質(zhì)金屬蛋白酶8(MMP8)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
SCA103Ra | 基質(zhì)金屬蛋白酶8(MMP8)檢測試劑盒(化學(xué)發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
LMA103Ra | 基質(zhì)金屬蛋白酶8(MMP8)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻
雜志 | 參考文獻 |
PLoS ONE | A Combinatorial Relative Mass Value Evaluation of Endogenous Bioactive Proteins in Three-Dimensional Cultured Nucleus Pulposus Cells of Herniated Intervertebral Discs: Identification of Potential Target Proteins for Gene Therapeutic Approaches [Plosone: Source] |
Journal of Periodontology | Effects of Photodynamic Therapy on the Clinical and Gingival Crevicular Fluid Inflammatory Biomarkers in Chronic Periodontitis: A Split-Mouth Randomized Clinical Trial [Joponline: 130464] |
Thromb Haemost. | Analysis of the expression of nine secreted matrix metalloproteinases and their endogenous inhibitors in the brain of mice subjected to ischaemic stroke [Pubmed:24671655] |
An Experimental Model for Inducing Periodontal Pathology in Rat: Histopathological and Enzymatic Aspects [Usamvcluj:Source] | |
Journal of Molecular and Cellular Cardiology | Treatment with anti-RANKL antibody reduces infarct size and attenuates dysfunction impacting on neutrophil-mediated injury [Pubmed:27056420] |
J Periodontal Implant Sci | Assessment of MMP-1, MMP-8 and TIMP-2 in experimental periodontitis treated with kaempferol [pmc:PMC4848383] |
Int Wound J. | From varices to venous ulceration: the story of chronic venous disease described by metalloproteinases. [Pubmed:26991748] |
Eur J Clin Invest.? | Alamandine abrogates neutrophil degranulation in atherosclerotic mice. [pubmed:27930810] |
International Wound Journal | From varices to venous ulceration: the story of chronic venous disease described by metalloproteinases [pubmed:26991748] |
Medicine (Baltimore) | Rapid detection of urinary soluble intercellular adhesion molecule-1 for determination of lupus nephritis activity [Pubmed:29953010] |
Scientific Reports | Atherosclerotic plaque vulnerability is increased in mouse model of lupus [Pubmed: 33110193] |
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