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27kDa熱休克蛋白(Hsp27)單克隆抗體

Monoclonal Antibody to Heat Shock Protein 27 (Hsp27)

HSPB1; HSP-B1; CMT2F; HSP28; Hsp25; Heat Shock 27kDa Protein 1; 28 kDa heat shock protein; Heat shock protein beta-1; Estrogen-regulated 24 kDa protein;

編號(hào)MAA693Hu21
物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 所有
- 人
- 小鼠
- 大鼠
- 豚鼠
- 家兔
- 獼猴
- 山羊
- 綿羊
- 馬
- 牛
- 豬
- 雞
- 狗
- 其他物種
- 多物種
- 泛物種
來源單抗制備
宿主小鼠
效價(jià)(克隆號(hào))c2
Ig亞型 IgG1 Kappa
純化方式蛋白A + 蛋白G親和純化
標(biāo)記物無標(biāo)記物
免疫原 RPA693Hu01-27kDa熱休克蛋白(Hsp27)重組蛋白
緩沖液成份0.01M 磷酸鹽緩沖液（pH7.4，containing 0.05% Proclin-300，50% glycerol）
性狀液體
濃度1mg/mL
且適物種Sus scrofa; Porcine (Pig，豬)
應(yīng)用WB; IHC; ICC; IP.
如果抗體需用于流式細(xì)胞術(shù)，請(qǐng)參見流式抗體。
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規(guī)格 20μl100μl 200μl 1ml 10ml
價(jià)格 ¥ 648 1512 ¥ 1512 ¥ 2160 ¥ 5400 ¥ 21600
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產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)

DAB staining on IHC-P; Sample: Human Glioma Tissue; Primary Ab: 30μg/ml Mouse Anti-Human Hsp27 Antibody Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
DAB staining on IHC-P;
Sample: Human Stomach Tissue;
Primary Ab: 30μg/ml Mouse Anti-Human Hsp27 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
DAB staining on IHC-P;
Sample: Human Prostate Tissue;
Primary Ab: 30μg/ml Mouse Anti-Human Hsp27 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
DAB staining on IHC-P;
Sample: Human Breast cancer Tissue;
Primary Ab: 30μg/ml Mouse Anti-Human Hsp27 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
DAB staining on IHC-P;
Sample: Human Prostate cancer Tissue;
Primary Ab: 30μg/ml Mouse Anti-Human Hsp27 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
DAB staining on IHC-P;
Sample: Human Skin cancer Tissue;
Primary Ab: 30μg/ml Mouse Anti-Human Hsp27 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
DAB staining on IHC-P;
Sample: Human Brain Tissue;
Primary Ab: 30μg/ml Mouse Anti-Human Hsp27 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
DAB staining on IHC-P;
Sample: Human Lung cancer Tissue;
Primary Ab: 30μg/ml Mouse Anti-Human Hsp27 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
DAB staining on IHC-P;
Sample: Porcine Liver Tissue
Primary Ab: 30μg/ml Mouse Anti-Human Hsp27 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)

Western Blot; Sample: Lane1: Human Lung lysate; Lane2: Porcine Skeletal muscle lysate; Lane3: Porcine Heart lysate Primary Ab: 2μg/ml Mouse Anti-Human Hsp27 Antibody Second Ab: 0.2μg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
Western Blot; Sample: Lane1: Porcine Heart lysate; Lane2: Porcine Skeletal muscle lysate; Lane3: Hela cell lysate; Lane4: HepG2 cell lysate
Primary Ab: 0.2μg/ml Mouse Anti-Human Hsp27 Antibody
Second Ab: 0.2μg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
Human MCF7 cell line was fixed with 2% paraformaldehyde (10 min) , permeabilised with 0.1% BSA-Triton X-100,then stained with 10μg/ml mouse Anti-human Hsp27 Monoclonal Antibody (Catalog MAA693Hu21, red histogram) or Isotype control antibody (Catalog IS067-Mu01, green histogram), followed by 1μg/ml FITC-conjugated Anti-mouse IgG Secondary Antibody (Catalog SAA544Mu18).

通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證

特異性

該抗體是針對(duì)Hsp27的小鼠單克隆抗體。在免疫組織化學(xué)染色和免疫印跡實(shí)驗(yàn)中能識(shí)別Hsp27。

用法

Western blottin:g: 0.5-3μg/mL;
Immunohistochemistry: 5-30μg/mL;
Immunocytochemistry: 5-30μg/mL;
Flow cytometry: 10μg/ml;
Optimal working dilutions must be determined by end user.

儲(chǔ)存

經(jīng)常使用則4°C保存。-20°C保存不超過兩年。避免反復(fù)凍融。

穩(wěn)定性

熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定，具體方法如下：在37°C孵育48小時(shí)，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲(chǔ)，損失率低于5%。

贈(zèng)品

增值服務(wù)

抗體標(biāo)記定制服務(wù)
Protein-A/G純化柱
 細(xì)胞和組織染色液
 抗體陽(yáng)性對(duì)照品
 組織/切片定制服務(wù)
磷酸化抗體定制服務(wù)
免疫印跡(WB)實(shí)驗(yàn)服務(wù)
免疫組織化學(xué)(IHC)實(shí)驗(yàn)服務(wù)
免疫細(xì)胞化學(xué)(ICC)實(shí)驗(yàn)服務(wù)
流式細(xì)胞術(shù)(FCM)實(shí)驗(yàn)服務(wù)
免疫沉淀(IP)實(shí)驗(yàn)服務(wù)
免疫熒光(IF)實(shí)驗(yàn)服務(wù)
緩沖液
 DAB顯色試劑盒
 SABC試劑盒
 長(zhǎng)臂生物素標(biāo)記試劑盒
 實(shí)時(shí)熒光定量PCR實(shí)驗(yàn)服務(wù)

相關(guān)產(chǎn)品

編號(hào)	適用物種：Homo sapiens (Human，人)	應(yīng)用(僅供研究使用，不用于臨床診斷！)
EPA693Hu61	27kDa熱休克蛋白(Hsp27)真核蛋白	Positive Control; Immunogen; SDS-PAGE; WB.
RPA693Hu01	27kDa熱休克蛋白(Hsp27)重組蛋白	Positive Control; Immunogen; SDS-PAGE; WB.
APA693Hu61	27kDa熱休克蛋白(Hsp27)活性蛋白	Cell?culture;?Activity?Assays.
PAA693Hu01	27kDa熱休克蛋白(Hsp27)多克隆抗體	WB; IHC; ICC/IF
FAA693Hu01	抗27kDa熱休克蛋白(Hsp27)多克隆抗體	FCM
LAA693Hu81	27kDa熱休克蛋白(Hsp27)多克隆抗體(異硫氰酸熒光素標(biāo)記)	WB; IHC; ICC; IF.
LAA693Hu71	27kDa熱休克蛋白(Hsp27)多克隆抗體(生物素標(biāo)記)	WB; IHC; ICC.
FAA693Hu81	抗27kDa熱休克蛋白(Hsp27)多克隆抗體(異硫氰酸熒光素標(biāo)記)	Flow cytometry.
MAA693Hu22	27kDa熱休克蛋白(Hsp27)單克隆抗體	WB; IHC; ICC; IP.
MAA693Hu21	27kDa熱休克蛋白(Hsp27)單克隆抗體	WB; IHC; ICC; IP.
MAA693Hu23	27kDa熱休克蛋白(Hsp27)單克隆抗體	WB; IHC
MAA693Hu24	27kDa熱休克蛋白(Hsp27)單克隆抗體	WB; IHC; ICC; IP.
MAA693Hu25	27kDa熱休克蛋白(Hsp27)單克隆抗體	WB; IHC; ICC; IP.
MAA693Hu27	27kDa熱休克蛋白(Hsp27)單克隆抗體	WB; IHC; ICC; IP.
MAA693Hu29	27kDa熱休克蛋白(Hsp27)單克隆抗體	WB; IHC; ICC; IP.
FAA693Hu06	抗27kDa熱休克蛋白(Hsp27)單克隆抗體	Flow cytometry.
FAA693Hu08	抗27kDa熱休克蛋白(Hsp27)單克隆抗體	Flow cytometry.
FAA693Hu04	抗27kDa熱休克蛋白(Hsp27)單克隆抗體	Flow cytometry.
FAA693Hu02	抗27kDa熱休克蛋白(Hsp27)單克隆抗體	Flow cytometry.
LAA693Hu74	27kDa熱休克蛋白(Hsp27)單克隆抗體(生物素標(biāo)記)	WB; IHC; ICC.
SEA693Hu	27kDa熱休克蛋白(Hsp27)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)	Enzyme-linked immunosorbent assay for Antigen Detection.
HEA693Hu	27kDa熱休克蛋白(Hsp27)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,高敏型)	Enzyme-linked immunosorbent assay for Antigen Detection.
LMA693Hu	27kDa熱休克蛋白(Hsp27)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法)	FLIA Kit for Antigen Detection.
KSA693Hu01	27kDa熱休克蛋白(Hsp27)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法)	自己動(dòng)手制作ELISA試劑盒的主要材料

參考文獻(xiàn)

雜志	參考文獻(xiàn)
Animal Science Journal	The effect of heat stress on gene expression and synthesis of heat‐shock and milk proteins in bovine mammary epithelial cells [PubMed: 26467738]
Nature?Communications	HSP27 is a partner of JAK2-STAT5 and a potential therapeutic target in myelofibrosis [Pubmed:29650953]
Advances in Experimental?Medicine?and?Biology	Molecules of Damage-Associated Patterns in Bronchoalveolar Lavage Fluid and Serum in Chronic Obstructive Pulmonary Disease [Pubmed:29429028]
Diabetes Research and Clinical Practice	The beneficial effects of 15 units of high-intensity circuit training in women is modified by age, baseline insulin resistance and physical capacity [Pubmed: 31102684]
Journal of Immunology Research	Damage-Associated Molecular Patterns and Myeloid-Derived Suppressor Cells in Bronchoalveolar Lavage Fluid in Chronic Obstructive Pulmonary Disease Patients []
Journal of Clinical Medicine	Damage-Associated Molecular Patterns and Th-Cell-Related Cytokines Released after Progressive Effort [Pubmed: 32210109]
Medicine	Regulatory T cells, damage-associated molecular patterns, and myeloid-derived suppressor cells in bronchoalveolar lavage fluid interlinked with chronic obstructive?… [Pubmed:35687771]
Beilstein Archives	Immunization of mice with gold nanoparticles conjugated to thermostable tumor antigens prevents tumor development during transplantation []

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