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27kDa熱休克蛋白(Hsp27)單克隆抗體

Monoclonal Antibody to Heat Shock Protein 27 (Hsp27)

HSPB1; HSP-B1; CMT2F; HSP28; Hsp25; Heat Shock 27kDa Protein 1; 28 kDa heat shock protein; Heat shock protein beta-1; Estrogen-regulated 24 kDa protein;

編號MAA693Hu23
物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 所有
- 人
- 小鼠
- 大鼠
- 豚鼠
- 家兔
- 獼猴
- 山羊
- 綿羊
- 馬
- 牛
- 豬
- 雞
- 狗
- 其他物種
- 多物種
- 泛物種
來源單抗制備
宿主小鼠
效價(克隆號)C1
Ig亞型 IgG1 Kappa
純化方式蛋白A + 蛋白G親和純化
標記物無標記物
免疫原 RPA693Hu01-27kDa熱休克蛋白(Hsp27)重組蛋白
緩沖液成份0.01M 磷酸鹽緩沖液（pH7.4，containing 0.05% Proclin-300，50% glycerol）
性狀液體
濃度1mg/ml
且適物種Sus scrofa; Porcine (Pig，豬)
應(yīng)用WB; IHC
如果抗體需用于流式細胞術(shù)，請參見流式抗體。
下載英文說明書中文說明書
規(guī)格 20μl100μl 200μl 1ml 10ml
價格 ¥ 648 1512 ¥ 1512 ¥ 2160 ¥ 5400 ¥ 21600
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產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)

DAB staining on IHC-P; Sample: Human Breast cancer Tissue; Primary Ab: 30μg/ml Mouse Anti-Human HSPB1 Antibody Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
DAB staining on IHC-P;
Sample: Human Glioma Tissue
Primary Ab: 30μg/ml Mouse Anti-Human Hsp27 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
DAB staining on IHC-P;
Sample: Human Lung cancer Tissue
Primary Ab: 30μg/ml Mouse Anti-Human Hsp27 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
DAB staining on IHC-P;
Sample: Porcine Liver Tissue
Primary Ab: 30μg/ml Mouse Anti-Human Hsp27 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)

Western Blot; Sample: Human Lung lysate; Primary Ab: 2μg/ml Mouse Anti-Human HSPB1 Antibody Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
Western Blot; Sample: Porcine Skeletal muscle lysate;
Primary Ab: 2μg/ml Mouse Anti-Human HSPB1 Antibody
Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
Western Blot; Sample: Porcine Heart lysate;
Primary Ab: 2μg/ml Mouse Anti-Human HSPB1 Antibody
Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
Western Blot; Sample: Lane1: Porcine Heart lysate; Lane2: Porcine Skeletal muscle lysate; Lane3: Hela cell lysate; Lane4: HepG2 cell lysate
Primary Ab: 0.2μg/ml Mouse Anti-Human Hsp27 Antibody
Second Ab: 0.2μg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
Human MCF7 cell line was fixed with 2% paraformaldehyde (10 min) , permeabilised with 0.1% BSA-Triton X-100,then stained with 10μg/ml mouse Anti-human Hsp27 Monoclonal Antibody (Catalog MAA693Hu23, red histogram) or Isotype control antibody (Catalog IS067-Mu01, green histogram), followed by 1μg/ml FITC-conjugated Anti-mouse IgG Secondary Antibody (Catalog SAA544Mu18).

通過ISO 9001、ISO 13485質(zhì)量體系認證

特異性

該抗體是針對Hsp27的小鼠單克隆抗體。在免疫組織化學染色和免疫印跡實驗中能識別Hsp27。

用法

Western blottin:g: 0.01-3μg/mL;
Immunohistochemistry: 5-30μg/mL;
Flow cytometry: 10μg/ml;
Optimal working dilutions must be determined by end user.

儲存

經(jīng)常使用則4°C保存。-20°C保存不超過兩年。避免反復(fù)凍融。

穩(wěn)定性

熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當?shù)臈l件下存儲，損失率低于5%。

贈品

增值服務(wù)

抗體標記定制服務(wù)
Protein-A/G純化柱
 細胞和組織染色液
 抗體陽性對照品
 組織/切片定制服務(wù)
磷酸化抗體定制服務(wù)
免疫印跡(WB)實驗服務(wù)
免疫組織化學(IHC)實驗服務(wù)
免疫細胞化學(ICC)實驗服務(wù)
流式細胞術(shù)(FCM)實驗服務(wù)
免疫沉淀(IP)實驗服務(wù)
免疫熒光(IF)實驗服務(wù)
緩沖液
 DAB顯色試劑盒
 SABC試劑盒
 長臂生物素標記試劑盒
 實時熒光定量PCR實驗服務(wù)

相關(guān)產(chǎn)品

編號	適用物種：Homo sapiens (Human，人)	應(yīng)用(僅供研究使用，不用于臨床診斷！)
EPA693Hu61	27kDa熱休克蛋白(Hsp27)真核蛋白	Positive Control; Immunogen; SDS-PAGE; WB.
RPA693Hu01	27kDa熱休克蛋白(Hsp27)重組蛋白	Positive Control; Immunogen; SDS-PAGE; WB.
APA693Hu61	27kDa熱休克蛋白(Hsp27)活性蛋白	Cell?culture;?Activity?Assays.
PAA693Hu01	27kDa熱休克蛋白(Hsp27)多克隆抗體	WB; IHC; ICC/IF
FAA693Hu01	抗27kDa熱休克蛋白(Hsp27)多克隆抗體	FCM
LAA693Hu81	27kDa熱休克蛋白(Hsp27)多克隆抗體(異硫氰酸熒光素標記)	WB; IHC; ICC; IF.
LAA693Hu71	27kDa熱休克蛋白(Hsp27)多克隆抗體(生物素標記)	WB; IHC; ICC.
FAA693Hu81	抗27kDa熱休克蛋白(Hsp27)多克隆抗體(異硫氰酸熒光素標記)	Flow cytometry.
MAA693Hu22	27kDa熱休克蛋白(Hsp27)單克隆抗體	WB; IHC; ICC; IP.
MAA693Hu21	27kDa熱休克蛋白(Hsp27)單克隆抗體	WB; IHC; ICC; IP.
MAA693Hu23	27kDa熱休克蛋白(Hsp27)單克隆抗體	WB; IHC
MAA693Hu24	27kDa熱休克蛋白(Hsp27)單克隆抗體	WB; IHC; ICC; IP.
MAA693Hu25	27kDa熱休克蛋白(Hsp27)單克隆抗體	WB; IHC; ICC; IP.
MAA693Hu27	27kDa熱休克蛋白(Hsp27)單克隆抗體	WB; IHC; ICC; IP.
MAA693Hu29	27kDa熱休克蛋白(Hsp27)單克隆抗體	WB; IHC; ICC; IP.
FAA693Hu06	抗27kDa熱休克蛋白(Hsp27)單克隆抗體	Flow cytometry.
FAA693Hu08	抗27kDa熱休克蛋白(Hsp27)單克隆抗體	Flow cytometry.
FAA693Hu04	抗27kDa熱休克蛋白(Hsp27)單克隆抗體	Flow cytometry.
FAA693Hu02	抗27kDa熱休克蛋白(Hsp27)單克隆抗體	Flow cytometry.
LAA693Hu74	27kDa熱休克蛋白(Hsp27)單克隆抗體(生物素標記)	WB; IHC; ICC.
SEA693Hu	27kDa熱休克蛋白(Hsp27)檢測試劑盒(酶聯(lián)免疫吸附試驗法)	Enzyme-linked immunosorbent assay for Antigen Detection.
HEA693Hu	27kDa熱休克蛋白(Hsp27)檢測試劑盒(酶聯(lián)免疫吸附試驗法,高敏型)	Enzyme-linked immunosorbent assay for Antigen Detection.
LMA693Hu	27kDa熱休克蛋白(Hsp27)等多因子檢測試劑盒(流式熒光發(fā)光法)	FLIA Kit for Antigen Detection.
KSA693Hu01	27kDa熱休克蛋白(Hsp27)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法)	自己動手制作ELISA試劑盒的主要材料

參考文獻

雜志	參考文獻
Animal Science Journal	The effect of heat stress on gene expression and synthesis of heat‐shock and milk proteins in bovine mammary epithelial cells [PubMed: 26467738]
Nature?Communications	HSP27 is a partner of JAK2-STAT5 and a potential therapeutic target in myelofibrosis [Pubmed:29650953]
Advances in Experimental?Medicine?and?Biology	Molecules of Damage-Associated Patterns in Bronchoalveolar Lavage Fluid and Serum in Chronic Obstructive Pulmonary Disease [Pubmed:29429028]
Diabetes Research and Clinical Practice	The beneficial effects of 15 units of high-intensity circuit training in women is modified by age, baseline insulin resistance and physical capacity [Pubmed: 31102684]
Journal of Immunology Research	Damage-Associated Molecular Patterns and Myeloid-Derived Suppressor Cells in Bronchoalveolar Lavage Fluid in Chronic Obstructive Pulmonary Disease Patients []
Journal of Clinical Medicine	Damage-Associated Molecular Patterns and Th-Cell-Related Cytokines Released after Progressive Effort [Pubmed: 32210109]
Medicine	Regulatory T cells, damage-associated molecular patterns, and myeloid-derived suppressor cells in bronchoalveolar lavage fluid interlinked with chronic obstructive?… [Pubmed:35687771]
Beilstein Archives	Immunization of mice with gold nanoparticles conjugated to thermostable tumor antigens prevents tumor development during transplantation []

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