神經(jīng)生長(zhǎng)因子(NGF)重組蛋白
Recombinant Nerve Growth Factor (NGF)
NGFB; Beta-NGF; HSAN5; NGF-B; Beta-Nerve Growth Factor
- 編號(hào)RPA105Si01
- 物種Rhesus monkey (Simian，恒河猴) 相同的名稱，不同的物種。
- 來源原核表達(dá)
- 宿主E.coli
- 內(nèi)毒素水平<1.0EU/µg(LAL法測(cè)定)
- 亞細(xì)胞定位分泌
- 預(yù)測(cè)分子量28.4kDa
- 實(shí)際分子量28kDa(差異分析請(qǐng)參閱說明書)
- 片段與標(biāo)簽Glu19~Arg121+DDDDK+Ser122~Arg239 with N-terminal His Tag
- 緩沖液成份20mM Tris, 150mM NaCl緩沖液（pH8.0, 含有1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose和Proclin300）
- 性狀凍干粉
- 純度> 97%
- 等電點(diǎn)9.8
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應(yīng)用
Positive Control; Immunogen; SDS-PAGE; WB.
如果需要有生物活性的蛋白，請(qǐng)參見活性蛋白。 - 下載 英文說明書 中文說明書
- 規(guī)格 10μg50μg 200μg 1mg 5mg
- 價(jià)格 ¥ 984 ¥ 2460 ¥ 4920 ¥ 14760 ¥ 36900
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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Figure . Gene Sequencing (extract)
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Figure. SDS-PAGE
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通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
序列
用法
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
儲(chǔ)存
避免反復(fù)凍融。2-8°C不超過一個(gè)月，-80°C不超過12個(gè)月。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定，具體方法如下：在37°C孵育48小時(shí)，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲(chǔ)，損失率低于5%。
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相關(guān)產(chǎn)品
| 編號(hào) | 適用物種：Rhesus monkey (Simian，恒河猴) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| RPA105Si01 | 神經(jīng)生長(zhǎng)因子(NGF)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA105Si01 | 神經(jīng)生長(zhǎng)因子(NGF)多克隆抗體 | WB; IHC; ICC; IP. |
| MAA105Si21 | 神經(jīng)生長(zhǎng)因子(NGF)單克隆抗體 | WB; IHC; ICC; IP. |
| SEA105Si | 神經(jīng)生長(zhǎng)因子(NGF)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA105Si | 神經(jīng)生長(zhǎng)因子(NGF)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| The Tohoku Journal of Experimental Medicine | Low-intensity aerobic exercise mitigates exercise-induced bronchoconstriction by improving the function of adrenal medullary chromaffin cells in asthmatic rats [Pubmed:25212248] |
| International Journal of Pharmaceutics | Neurotrophic and antioxidant effects of silymarin comparable to 4-methylcatechol in protection against gentamicin-induced ototoxicity in guinea pigs [Pubmed:25445537] |
| Yonsei Medical Journal | NGF and HB-EGF: Potential Biomarkers that Reflect the Effects of Fesoterodine in Patients with Overactive Bladder Syndrome [Pubmed:25510766] |
| Molecular and Cellular Endocrinology | High glucose upregulates CYP24A1 expression which attenuates the ability of 1,25(OH)2D3 to increase NGF secretion in a rat Schwann cell line RSC96 [Pubmed:25614971] |
| Mol Cell Endocrinol | High glucose upregulates CYP24A1 expression which attenuates the ability of 1,25(OH)2D3 [PubMed: 25614971] |
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| Psychoneuroendocrinology | Chronic mild stress influences nerve growth factor through a matrix metalloproteinase-dependent mechanism [Pubmed:26771945] |
| Genet Mol Res | Changes in growth factor levels in the cerebrospinal fluid of autism patients after transplantation of human umbilical cord blood mononuclear cells and umbilical cord-derived mesenchymal stem cells [Pubmed:27323064] |
| The Journal of Maternal-Fetal & Neonatal Medicine | Differential expression of cord blood neurotrophins in gestational diabetes: the impact of fetal growth abnormalities [pubmed:28081639] |
| IOS Press Content Library | An Intranasal Formulation of Erythropoietin (Neuro-EPO) Prevents Memory Deficits and Amyloid Toxicity in the APPSwe Transgenic Mouse Model of Alzheimer's … [articles:journal-of-alzheimers-disease] |
| biblioteca.uva | Evaluación de la secreción de factores neurotróficos por las células MSV en cocultivo con neurorretinas porcinas, en función de la dosis celular y del tiempo de cultivo [10324/27440] |
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| Biochemical Pharmacology | Effects of n-3 PUFA enriched and n-3 PUFA deficient diets in na?ve and Aβ-treated female rats [Pubmed:30028991] |
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| Experimental and Therapeutic Medicine | Involvement of neurotrophic signaling in doxorubicin?induced cardiotoxicity [Pubmed: 32010279] |
| Open Access Macedonian Journal of Medical Sciences | Inhibition of Receptor for Advanced Glycation End Products as New Promising Strategy Treatment in Diabetic Retinopathy [Pubmed: 32165929] |
| MOLECULAR NUTRITION & FOOD RESEARCH | Hyperlipidemia Down‐Regulate Brain Antioxidant Defense Enzymes and Neurotrophins in Rats: Assessment of the Modulatory Potential of EPA+ DHA and?… [Pubmed: 32918393] |
| Neuropharmacology | Impaired LTD-like motor cortical plasticity in female patients with major depression disorder [Pubmed: 32791084] |
| COLLOIDS AND SURFACES B-BIOINTERFACES | Effects of gold nanoparticles administration through behavioral and oxidative parameters in animal model of parkinson's disease [Pubmed: 32777662] |
| 博士論文 | Neurobiological determinants of depressive-like symptoms in rodents [] |
| Medical Research Journal | Changes of substance P, NGF and CGRP salivary levels among patients undergoing physical therapy [] |
| Journal of the Taiwan Institute of Chemical Engineers | Multiple-component dual-phase solid lipid nanoparticles with conjugated transferrin for formulating antioxidants and nerve growth factor against neuronal apoptosis [] |
| EUROPEAN CHILD & ADOLESCENT PSYCHIATRY | Increased serum brain-derived neurotrophic factor and adrenocorticotropic hormone levels are associated with obsessive compulsive disorder in medication?free??- [33389158] |
| DRUG DEVELOPMENT RESEARCH | Neuroprotective effect of Na+/H+?exchangers isoform?\1 inactivation against 6?\hydroxydopamine?\induced mitochondrial dysfunction and neuronal apoptosis in Parkinson's disease models [33538000] |
| BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | Sea urchin gangliosides exhibit neuritogenic effects in neuronal PC12 cells via TrkA- and TrkB-related pathways [33589896] |
| Neuropsychiatr Dis Treat | Repetitive Transcranial Magnetic Stimulation Improves Mild Cognitive Impairment Associated with Alzheimer's Disease in Mice by Modulating the miR-567?… [34239303] |
| Pharmaceuticals (Basel) | Carissa macrocarpa Leaves Polar Fraction Ameliorates Doxorubicin-Induced Neurotoxicity in Rats via Downregulating the Oxidative Stress and Inflammatory Markers [34959705] |
| Cell Biochem Funct | CNTs‐CaP/chitosan‐coated AZ91D magnesium alloy extract promoted rat dorsal root ganglia neuron growth via activating ERK signalling pathway [34296452] |
| Carbohydrate Polymers | Gene delivery of chitosan-graft-polyethyleneimine vectors loaded on scaffolds for nerve regeneration [Pubmed:35550777] |
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