骨粘連蛋白(ON)重組蛋白
Recombinant Osteonectin (ON)
SPARC; BM-40; Secreted Protein,Acidic,Cysteine-Rich; Basement-membrane protein 40; Secreted protein acidic and rich in cysteine
- 編號RPA791Ra01
- 物種Rattus norvegicus (Rat,大鼠) 相同的名稱,不同的物種。
- 來源原核表達(dá)
- 宿主E.coli
- 內(nèi)毒素水平<1.0EU/µg(LAL法測定)
- 亞細(xì)胞定位胞漿
- 預(yù)測分子量33.5kDa
- 實際分子量40kDa(差異分析請參閱說明書)
- 片段與標(biāo)簽Ala18~Ile301 with N-terminal His Tag
- 緩沖液成份20mM Tris, 150mM NaCl緩沖液(pH8.0, 含有1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose和Proclin300)
- 性狀凍干粉
- 純度> 90%
- 等電點4.7
-
應(yīng)用
Positive Control; Immunogen; SDS-PAGE; WB.
如果需要有生物活性的蛋白,請參見活性蛋白。 - 下載 英文說明書 中文說明書
- 規(guī)格 10μg50μg 200μg 1mg 5mg
- 價格 ¥ 912 ¥ 2280 ¥ 4560 ¥ 13680 ¥ 34200
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序列
用法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
儲存
避免反復(fù)凍融。2-8°C不超過一個月,-80°C不超過12個月。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定,具體方法如下:在37°C孵育48小時,沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi),在適當(dāng)?shù)臈l件下存儲,損失率低于5%。
增值服務(wù)
相關(guān)產(chǎn)品
編號 | 適用物種:Rattus norvegicus (Rat,大鼠) | 應(yīng)用(僅供研究使用,不用于臨床診斷!) |
RPA791Ra01 | 骨粘連蛋白(ON)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
PAA791Ra01 | 骨粘連蛋白(ON)多克隆抗體 | WB; IHC; ICC; IP. |
LAA791Ra71 | 骨粘連蛋白(ON)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
LAA791Ra81 | 骨粘連蛋白(ON)多克隆抗體(異硫氰酸熒光素標(biāo)記) | WB; IHC; ICC; IF. |
MAA791Ra21 | 骨粘連蛋白(ON)單克隆抗體 | WB; IHC; ICC; IP. |
SEA791Ra | 骨粘連蛋白(ON)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
LMA791Ra | 骨粘連蛋白(ON)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
KSA791Ra01 | 骨粘連蛋白(ON)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻(xiàn)
雜志 | 參考文獻(xiàn) |
J Mol Med (Berl) | Growth hormone replacement therapy regulates microRNA-29a and targets involved in insulin resistance [PubMed: 26199111] |
Muscle Nerve | Effect of resistance ladder training on SPARC expression in skeletal muscle of hindlimb immobilized rats [PubMed: 26467437] |
Medicine (Baltimore) | An Attempt to Evaluate Selected Aspects of “Bone–Fat Axis” Function in Healthy Individuals and Patients With Pancreatic Cancer [PubMed: 26266370] |
British Journal of Cancer | A proteomics-based approach identifies secreted protein acidic and rich in cysteine as a prognostic biomarker in malignant pleural mesothelioma [Pubmed:26889976] |
Journal of diabetes and its complications | Associations between FGF21, osteonectin and bone turnover markers in type 2 diabetic patients with albuminuria. [pubmed:27916484] |
American journal of the medical sciences | Association of Bone Metabolic Markers With Diabetic Retinopathy and Diabetic Macular Edema in Elderly Chinese Individuals With Type 2 Diabetes Mellitus [pubmed:29078839] |
Translational Research in Oral Oncology | Testican 1 (SPOCK1) and protein tyrosine phosphatase, receptor type S (PTPRS) show significant increase in saliva of tobacco users with oral cancer [Doi: 10.1177/2057178X18800534] |
[ENGINEERING OF BIOMATERIALS | Osteoconductive potential of PLGA/bioglass composite biomaterials [] |
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