非神經(jīng)元性烯醇化酶(ENO1)多克隆抗體
Polyclonal Antibody to Enolase 1 (ENO1)
NNE; ENO1L1; MPB1; PPH; Alpha Enolase; Enolase 1; Phosphopyruvate hydratase; Plasminogen-binding protein; 2-phospho-D-glycerate hydro-lyase; C-myc promoter-binding
- 編號PAB449Hu02
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 來源多抗制備
- 宿主兔
- 效價-
- Ig類型 IgG
- 純化方式抗原特異性親和純化
- 標(biāo)記物無標(biāo)記物
- 免疫原 RPB449Hu02-非神經(jīng)元性烯醇化酶(ENO1)重組蛋白
- 緩沖液成份磷酸鹽緩沖液（pH7.4, 含有 0.01% SKL, 1mM DTT, 5% Trehalose和Proclin300.）
- 性狀液體
- 濃度500μg/mL
- 且適物種Mus musculus (Mouse，小鼠), Rattus norvegicus (Rat，大鼠)
- 應(yīng)用WB; IHC; ICC; IP.
如果抗體需用于流式細(xì)胞術(shù)，請參見流式抗體。 - 下載 英文說明書 中文說明書
- 規(guī)格 20μl100μl 200μl 1ml 10ml
- 價格 ¥ 598 ¥ 1394 ¥ 1992 ¥ 4980 ¥ 19920
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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IHC圖
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Figure. Western Blot; Sample: Recombinant ENO1, Human.
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Western Blot; Sample: Human Lung lysate;
Primary Ab: 1μg/ml Rabbit Anti-Human NNE Antibody
Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Western Blot; Sample: Human Serum;
Primary Ab: 1μg/ml Rabbit Anti-Human NNE Antibody
Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Western Blot; Samples: Lane1: Mouse Cerebrum lysate; Lane2: LO2 cell lysate; Lane3: PC3 cell lysate; Lane4: SGC7901 cell lysate;
Primary Ab: 0.05μg/ml Rabbit Anti-Human ENO1 Antibody
Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
WB圖
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WB圖
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通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
該抗體是針對ENO1的兔多克隆抗體。在免疫組織化學(xué)染色和免疫印跡實驗中能識別ENO1。
用法
免疫印跡：0.2-2μg/mL;1:250-2500
免疫組織化學(xué)：5-20μg/mL;1:25-100
免疫細(xì)胞化學(xué)：5-20μg/mL;1:25-100
最佳稀釋倍數(shù)最終由用戶決定。
儲存
經(jīng)常使用則4°C保存。-20°C保存不超過兩年。避免反復(fù)凍融。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲，損失率低于5%。
贈品
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| RPB449Hu03 | 非神經(jīng)元性烯醇化酶(ENO1)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPB449Hu02 | 非神經(jīng)元性烯醇化酶(ENO1)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPB449Hu01 | 非神經(jīng)元性烯醇化酶(ENO1)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAB449Hu02 | 非神經(jīng)元性烯醇化酶(ENO1)多克隆抗體 | WB; IHC; ICC; IP. |
| PAB449Hu03 | 非神經(jīng)元性烯醇化酶(ENO1)多克隆抗體 | WB; IHC; ICC; IP. |
| PAB449Hu01 | 非神經(jīng)元性烯醇化酶(ENO1)多克隆抗體 | WB; IHC; ICC; IP. |
| LAB449Hu71 | 非神經(jīng)元性烯醇化酶(ENO1)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| MAB449Hu21 | 非神經(jīng)元性烯醇化酶(ENO1)單克隆抗體 | WB; IHC; ICC; IP. |
| MAB449Hu22 | 非神經(jīng)元性烯醇化酶(ENO1)單克隆抗體 | WB; IHC. |
| SEB449Hu | 非神經(jīng)元性烯醇化酶(ENO1)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| AEB449Hu | 抗非神經(jīng)元性烯醇化酶抗體(Anti-ENO1)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antibody Detection. |
| SCB449Hu | 非神經(jīng)元性烯醇化酶(ENO1)檢測試劑盒(化學(xué)發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
參考文獻
| 雜志 | 參考文獻 |
| Analytical Chemistry | Diagnostic detection of human lung cancer-associated antigen using a gold nanoparticle-based electrochemical immunosensor [PubMed: 20557064] |
| Translational Research | Proteomic analysis of fine-needle aspiration in differential diagnosis of thyroid nodules [Pubmed:27172385] |
| Journal of?Proteomics | Putative salivary biomarkers useful to differentiate patients with fibromyalgia [Pubmed:29654921] |
| BioMed Research International | The Diagnostic Accuracy of Combined Enolase/Cr, CA125, and CA19-9 in the Detection of Endometriosis [Pubmed: 33062681] |
| Cancer Cell International | Bufalin Induced Mitochondrial Dysfunction Promotes Apoptosis of Glioma Cells by Regulating Annexin A2 and DRP1 Proteins [] |
| Cancer Cell Int | Bufalin induces mitochondrial dysfunction and promotes apoptosis of glioma cells by regulating Annexin A2 and DRP1 protein expression [34376212] |
| Clin Proteomics | Serum proteomics unveil characteristic protein diagnostic biomarkers and signaling pathways in patients with esophageal squamous cell carcinoma [Pubmed:35610567] |
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