Ⅰ型膠原α2(COL1a2)重組蛋白
Recombinant Collagen Type I Alpha 2 (COL1a2)
COL1-A2; OI4; Collagen Alpha-2(I)chain; osteogenesis imperfecta type IV; Collagen Of Skin,Tendon And bone,Alpha-2 Chain
- 編號RPA215Ga02
- 物種Chicken (Gallus,雞) 相同的名稱,不同的物種。
- 來源原核表達
- 宿主E.coli
- 內(nèi)毒素水平<1.0EU/µg(LAL法測定)
- 亞細胞定位分泌, 細胞外基質(zhì)
- 預測分子量63.0kDa
- 實際分子量70&33&22kDa(差異分析請參閱說明書)
- 片段與標簽Gln78~Pro443 with N-terminal His and GST Tag
- 緩沖液成份PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
- 性狀凍干粉
- 純度> 80%
- 等電點10.8
-
應(yīng)用
Positive Control; Immunogen; SDS-PAGE; WB.
如果需要有生物活性的蛋白,請參見活性蛋白。 - 下載 英文說明書 中文說明書
- 規(guī)格 10μg50μg 200μg 1mg 5mg
- 價格 ¥ 996 ¥ 2490 ¥ 4980 ¥ 14940 ¥ 37350
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序列
用法
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
儲存
避免反復凍融。2-8°C不超過一個月,-80°C不超過12個月。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定,具體方法如下:在37°C孵育48小時,沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi),在適當?shù)臈l件下存儲,損失率低于5%。
增值服務(wù)
相關(guān)產(chǎn)品
編號 | 適用物種:Chicken (Gallus,雞) | 應(yīng)用(僅供研究使用,不用于臨床診斷!) |
APA215Ga61 | Ⅰ型膠原α2(COL1a2)活性蛋白 | Cell?culture;?Activity?Assays. |
RPA215Ga02 | Ⅰ型膠原α2(COL1a2)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
RPA215Ga01 | Ⅰ型膠原α2(COL1a2)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
PAA215Ga01 | Ⅰ型膠原α2(COL1a2)多克隆抗體 | WB; IHC; ICC; IP. |
參考文獻
雜志 | 參考文獻 |
Plos one | Inhibition of TGF-β Signaling Enables Human Corneal Endothelial Cell Expansion In Vitro for Use in Regenerative Medicine [PubMed: PMC3581499] |
J Proteomics | Characterization of secretomes from a human blood brain barrier endothelial cells in-vitro model after ischemia by stable isotope labeling with aminoacids in cell culture (SILAC) [PubMed: 26718731] |
PLoS One | Pro-fibrotic compounds induce stellate cell activation, ECM-remodelling and Nrf2 activation in a human 3D-multicellular model of liver fibrosis [pubmed:28665955] |
American Journal of Translational Research | A novel hybrid 3D-printed titanium scaffold for osteogenesis in a rabbit calvarial defect model [Pubmed:29511441] |
Sci Rep | The effect of hypoxia on the proteomic signature of pig adipose-derived stromal/stem cells (pASCs) [Pubmed: 33208768] |
Experimental and Therapeutic Medicine | Protective effects of halophyte complex extract against UVB?induced damage in human keratinocytes and the skin of hairless mice [33986847] |
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