沉默調(diào)節(jié)蛋白3(SIRT3)重組蛋白
Recombinant Sirtuin 3 (SIRT3)
SIR2L3; Silent Mating Type Information Regulation 2 Homolog 3; NAD-dependent protein deacetylase sirtuin-3, mitochondrial; SIR2-like protein 3; Regulatory protein SIR2 homolog 3
- 編號(hào)RPE913Hu01
- 物種Homo sapiens (Human,人) 相同的名稱,不同的物種。
- 來(lái)源原核表達(dá)
- 宿主E.coli
- 內(nèi)毒素水平<1.0EU/µg(LAL法測(cè)定)
- 亞細(xì)胞定位線粒體
- 預(yù)測(cè)分子量31.7kDa
- 實(shí)際分子量32kDa(差異分析請(qǐng)參閱說(shuō)明書(shū))
- 片段與標(biāo)簽Gln126~Lys399 with N-terminal His Tag
- 緩沖液成份20mM Tris, 150mM NaCl緩沖液(pH8.0, 含有1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose和Proclin300)
- 性狀凍干粉
- 純度> 90%
- 等電點(diǎn)6.0
-
應(yīng)用
Positive Control; Immunogen; SDS-PAGE; WB.
如果需要有生物活性的蛋白,請(qǐng)參見(jiàn)活性蛋白。 - 下載 英文說(shuō)明書(shū) 中文說(shuō)明書(shū)
- 規(guī)格 10μg50μg 200μg 1mg 5mg
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序列
用法
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
儲(chǔ)存
避免反復(fù)凍融。2-8°C不超過(guò)一個(gè)月,-80°C不超過(guò)12個(gè)月。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定,具體方法如下:在37°C孵育48小時(shí),沒(méi)有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi),在適當(dāng)?shù)臈l件下存儲(chǔ),損失率低于5%。
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相關(guān)產(chǎn)品
編號(hào) | 適用物種:Homo sapiens (Human,人) | 應(yīng)用(僅供研究使用,不用于臨床診斷!) |
APE913Hu01 | 沉默調(diào)節(jié)蛋白3(SIRT3)活性蛋白 | Cell?culture;?Activity?Assays. |
RPE913Hu01 | 沉默調(diào)節(jié)蛋白3(SIRT3)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
PAE913Hu01 | 沉默調(diào)節(jié)蛋白3(SIRT3)多克隆抗體 | WB; IHC; ICC; IP. |
LAE913Hu81 | 沉默調(diào)節(jié)蛋白3(SIRT3)多克隆抗體(異硫氰酸熒光素標(biāo)記) | WB; IHC; ICC; IF. |
LAE913Hu71 | 沉默調(diào)節(jié)蛋白3(SIRT3)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
MAE913Hu21 | 沉默調(diào)節(jié)蛋白3(SIRT3)單克隆抗體 | WB,IHC |
MAE913Hu22 | 沉默調(diào)節(jié)蛋白3(SIRT3)單克隆抗體 | WB; IHC |
MAE913Hu23 | 沉默調(diào)節(jié)蛋白3(SIRT3)單克隆抗體 | WB; IHC; ICC; IP. |
LAE913Hu42 | 沉默調(diào)節(jié)蛋白3(SIRT3)單克隆抗體(藻紅素標(biāo)記) | WB; IHC; ICC; IF. |
SEE913Hu | 沉默調(diào)節(jié)蛋白3(SIRT3)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
LME913Hu | 沉默調(diào)節(jié)蛋白3(SIRT3)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻(xiàn)
雜志 | 參考文獻(xiàn) |
Toxicology?Letters | SirT3 regulates diabetogenic effects caused by arsenic: An implication for mitochondrial complex II modification [Pubmed: 30385301] |
International Breastfeeding Journal | Effects of perinatal factors on sirtuin 3, 8-hydroxy-2′-deoxyguanosine, brain-derived neurotrophic factor and serotonin in cord blood and early breast milk: an … [Pubmed: 32552911] |
Antioxidants | The Effect of Repeated Whole-Body Cryotherapy on Sirt1 and Sirt3 Concentrations and Oxidative Status in Older and Young Men Performing Different Levels of??- [33396247] |
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