70kDa熱休克蛋白1A(HSPA1A)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法)
ELISA Kit DIY Materials for Heat Shock 70kDa Protein 1A (HSPA1A)
HSP70-1; HSP-A1A; HSPA1; HSP70-2; HSX70
- 編號KSB081Hu01
- 物種Homo sapiens (Human,人) 相同的名稱,不同的物種。
- 試劑內(nèi)容 包被抗體、檢測抗體、標(biāo)準(zhǔn)品、HRP標(biāo)記的鏈霉親和素、TMB底物、96孔板
- 可檢測樣本Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 可適用原理雙抗夾心酶聯(lián)免疫吸附試驗法測抗原
- 可檢測范圍1.56-100ng/mL
- 可適靈敏度0.61ng/mL
- 應(yīng)用Main materials for "Do It (ELISA Kit) Yourself".
- 下載 英文說明書 中文說明書
- 規(guī)格 96T*596T*10 96T*20 96T*50 96T*100
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特異性
試劑盒中的抗體對70kDa熱休克蛋白1A(HSPA1A)具有高靈敏度和高特異性,經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
用法
1.用100μL每孔的工作濃度的包被抗體包被96孔板,4度孵育過夜或者37度孵育2小時;
2.洗板1次;
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時;
4.洗板1次。酶標(biāo)板即可用于ELISA實驗,后續(xù)使用方法與ELISA同。
儲存
抗體,標(biāo)準(zhǔn)品和HRP標(biāo)記的鏈霉親和素在-20度保存,TMB需在4度保存,96孔板在室溫保存。所有組分保質(zhì)期為1年,試劑一旦開封,在4度保存時間為1個月。
配套試劑
增值服務(wù)
相關(guān)產(chǎn)品
編號 | 適用物種:Homo sapiens (Human,人) | 應(yīng)用(僅供研究使用,不用于臨床診斷!) |
RPB081Hu02 | 70kDa熱休克蛋白1A(HSPA1A)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
RPB081Hu01 | 70kDa熱休克蛋白1A(HSPA1A)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
CAB081Hu01 | 抗70kDa熱休克蛋白1A(HSPA1A)多克隆抗體 | Loading Control of WB. |
PAB081Hu01 | 70kDa熱休克蛋白1A(HSPA1A)多克隆抗體 | WB; IHC; ICC; IP; FCM |
PAB081Hu02 | 70kDa熱休克蛋白1A(HSPA1A)多克隆抗體 | WB,IHC |
FAB081Hu03 | 抗70kDa熱休克蛋白1A(HSPA1A)多克隆抗體 | FCM |
MAB081Hu21 | 70kDa熱休克蛋白1A(HSPA1A)單克隆抗體 | WB; IHC; ICC/IF |
MAB081Hu25 | 70kDa熱休克蛋白1A(HSPA1A)單克隆抗體 | WB |
CAB081Hu22 | 抗70kDa熱休克蛋白1A(HSPA1A)單克隆抗體 | Loading Control of WB. |
MAB081Hu22 | 70kDa熱休克蛋白1A(HSPA1A)單克隆抗體 | WB |
FAB081Hu02 | 抗70kDa熱休克蛋白1A(HSPA1A)單克隆抗體 | Flow cytometry. |
HEB081Hu | 70kDa熱休克蛋白1A(HSPA1A)檢測試劑盒(酶聯(lián)免疫吸附試驗法,高敏型) | Enzyme-linked immunosorbent assay for Antigen Detection. |
SEB081Hu | 70kDa熱休克蛋白1A(HSPA1A)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
LMB081Hu | 70kDa熱休克蛋白1A(HSPA1A)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
KSB081Hu01 | 70kDa熱休克蛋白1A(HSPA1A)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
CGB081Hu01 | 70kDa熱休克蛋白1A(HSPA1A)cDNA | PCR、蛋白表達(dá)等 |
參考文獻(xiàn)
雜志 | 參考文獻(xiàn) |
Journal of Neuroinflammation | Identification of potential biomarkers of gold nanoparticle toxicity in rat brains [PubMed: 22691312] |
Mediators Inflamm.? | Local Inflammation in Fracture Hematoma: Results from a Combined Trauma Model in Pigs [Pubmed:Pmc4324980] |
Eur J Pharmacol. | Differential modulatory effects of morphine on acute and chronic stress induced neurobehavioral and cellular markers in rats [Pubmed:24508522] |
Pharmacol Rep. | Effect of propranolol on IL-10, visfatin, Hsp70, iNOS, TLR2, and survivin in amelioration of tumor progression and survival in Solid Ehrlich Carcinoma-bearing mice [Pubmed:25443743] |
Advances in Medical Sciences | A similar pro/anti-inflammatory cytokine balance is present in the airways of competitive athletes and non-exercising asthmatics [pubmed:28822267] |
Archives of Medical Research | Winter ambient training conditions are associated with increased bronchial hyperreactivity and with shifts in serum innate immunity proteins in young?… [Pubmed:29379533] |
metabolic brain disease | Curcumin attenuates cytoplasmic/endoplasmic reticulum stress, apoptosis and cholinergic dysfunction in diabetic rat hippocampus [Pubmed: 32172517] |
EXPERIMENTAL CELL RESEARCH | Antagomirs targeting miR-142–5p attenuate pilocarpine-induced status epilepticus in mice [Pubmed: 32439493] |
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