70kDa熱休克蛋白1A(HSPA1A)多克隆抗體
Polyclonal Antibody to Heat Shock 70kDa Protein 1A (HSPA1A)
HSP70-1; HSP-A1A; HSPA1; HSP70-2; HSX70
- 編號PAB081Hu02
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 來源多抗制備
- 宿主兔
- 效價-
- Ig類型 IgG
- 純化方式抗原特異性親和純化
- 標(biāo)記物無標(biāo)記物
- 免疫原 RPB081Hu02-70kDa熱休克蛋白1A(HSPA1A)重組蛋白
- 緩沖液成份0.01M 磷酸鹽緩沖液（pH7.4，containing 0.05% Proclin-300，50% glycerol）
- 性狀液體
- 濃度500μg/mL
- 且適物種Mus musculus (Mouse，小鼠), Sus scrofa; Porcine (Pig，豬)
- 應(yīng)用WB,IHC
如果抗體需用于流式細(xì)胞術(shù)，請參見流式抗體。 - 下載 英文說明書 中文說明書
- 規(guī)格 20μl100μl 200μl 1ml 10ml
- 價格 ¥ 598 ¥ 1394 ¥ 1992 ¥ 4980 ¥ 19920
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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DAB staining on IHC-P; Samples: Human Stomach Tissue; Primary Ab: 10μg/ml Rabbit Anti-Human HSPA1A Antibody Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
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DAB staining on IHC-P;
Samples: Human Brain Tissue;
Primary Ab: 10μg/ml Rabbit Anti-Human HSPA1A Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Samples: Human Prostate Tissue;
Primary Ab: 10μg/ml Rabbit Anti-Human HSPA1A Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Samples: Human Kidney Tissue;
Primary Ab: 10μg/ml Rabbit Anti-Human HSPA1A Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Samples: Human Breast cancer Tissue;
Primary Ab: 10μg/ml Rabbit Anti-Human HSPA1A Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Samples: Human Prostate cancer Tissue;
Primary Ab: 10μg/ml Rabbit Anti-Human HSPA1A Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
AF488 staining on IF;
Sample: Hela cell
Primary Ab: 20μg/ml Rabbit Anti-Human HSPA1A Antibody
Second Ab: 2μg/ml AF488-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb11)
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Figure. Western Blot; Sample: Recombinant HSPA1A, Human.
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Western Blot; Sample: Lane1: Porcine Liver lysate; Lane2: Porcine Cerebrum lysate; Lane3: Mouse Liver lysate; Lane4: A549 cell lysate
Primary Ab: 3μg/ml Rabbit Anti-Human HSPA1A Antibody
Second Ab: 0.2μg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Knockout Varification: <br/>Lane 1: Wild-type A549 cell lysate; <br/>Lane 2: HSPA1A knockout A549 cell lysate; <br/>Predicted MW: 64,70kDa <br/>Observed MW: 64kDa<br/>Primary Ab: 3μg/ml Rabbit Anti-Human HSPA1A Antibody<br/>Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody<br/>(Catalog: SAA544Rb19)
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通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
該抗體是針對HSPA1A的兔多克隆抗體。在免疫組織化學(xué)染色和免疫印跡實驗中能識別HSPA1A。
用法
Western blotting: 0.5-3μg/mL;
Immunohistochemistry: 5-20μg/mL;
Immunocytochemistry: 5-20μg/mL;
Optimal working dilutions must be determined by end user.
儲存
經(jīng)常使用則4°C保存。-20°C保存不超過兩年。避免反復(fù)凍融。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲，損失率低于5%。
贈品
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| RPB081Hu02 | 70kDa熱休克蛋白1A(HSPA1A)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPB081Hu01 | 70kDa熱休克蛋白1A(HSPA1A)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| CAB081Hu01 | 抗70kDa熱休克蛋白1A(HSPA1A)多克隆抗體 | Loading Control of WB. |
| PAB081Hu01 | 70kDa熱休克蛋白1A(HSPA1A)多克隆抗體 | WB; IHC; ICC; IP; FCM |
| PAB081Hu02 | 70kDa熱休克蛋白1A(HSPA1A)多克隆抗體 | WB,IHC |
| FAB081Hu03 | 抗70kDa熱休克蛋白1A(HSPA1A)多克隆抗體 | FCM |
| MAB081Hu21 | 70kDa熱休克蛋白1A(HSPA1A)單克隆抗體 | WB; IHC; ICC/IF |
| MAB081Hu25 | 70kDa熱休克蛋白1A(HSPA1A)單克隆抗體 | WB |
| CAB081Hu22 | 抗70kDa熱休克蛋白1A(HSPA1A)單克隆抗體 | Loading Control of WB. |
| MAB081Hu22 | 70kDa熱休克蛋白1A(HSPA1A)單克隆抗體 | WB |
| FAB081Hu02 | 抗70kDa熱休克蛋白1A(HSPA1A)單克隆抗體 | Flow cytometry. |
| HEB081Hu | 70kDa熱休克蛋白1A(HSPA1A)檢測試劑盒(酶聯(lián)免疫吸附試驗法,高敏型) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SEB081Hu | 70kDa熱休克蛋白1A(HSPA1A)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMB081Hu | 70kDa熱休克蛋白1A(HSPA1A)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| KSB081Hu01 | 70kDa熱休克蛋白1A(HSPA1A)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
| CGB081Hu01 | 70kDa熱休克蛋白1A(HSPA1A)cDNA | PCR、蛋白表達等 |
參考文獻
| 雜志 | 參考文獻 |
| Journal of Neuroinflammation | Identification of potential biomarkers of gold nanoparticle toxicity in rat brains [PubMed: 22691312] |
| Mediators Inflamm.? | Local Inflammation in Fracture Hematoma: Results from a Combined Trauma Model in Pigs [Pubmed:Pmc4324980] |
| Eur J Pharmacol. | Differential modulatory effects of morphine on acute and chronic stress induced neurobehavioral and cellular markers in rats [Pubmed:24508522] |
| Pharmacol Rep. | Effect of propranolol on IL-10, visfatin, Hsp70, iNOS, TLR2, and survivin in amelioration of tumor progression and survival in Solid Ehrlich Carcinoma-bearing mice [Pubmed:25443743] |
| Advances in Medical Sciences | A similar pro/anti-inflammatory cytokine balance is present in the airways of competitive athletes and non-exercising asthmatics [pubmed:28822267] |
| Archives of Medical Research | Winter ambient training conditions are associated with increased bronchial hyperreactivity and with shifts in serum innate immunity proteins in young?… [Pubmed:29379533] |
| metabolic brain disease | Curcumin attenuates cytoplasmic/endoplasmic reticulum stress, apoptosis and cholinergic dysfunction in diabetic rat hippocampus [Pubmed: 32172517] |
| EXPERIMENTAL CELL RESEARCH | Antagomirs targeting miR-142–5p attenuate pilocarpine-induced status epilepticus in mice [Pubmed: 32439493] |
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