70kDa熱休克蛋白1A(HSPA1A)單克隆抗體
Monoclonal Antibody to Heat Shock 70kDa Protein 1A (HSPA1A)
HSP70-1; HSP-A1A; HSPA1; HSP70-2; HSX70
- 編號MAB081Hu21
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 來源單抗制備
- 宿主小鼠
- 效價(克隆號)2#
- Ig亞型 IgG2b Kappa
- 純化方式蛋白A + 蛋白G親和純化
- 標(biāo)記物無標(biāo)記物
- 免疫原 RPB081Hu01-70kDa熱休克蛋白1A(HSPA1A)重組蛋白
- 緩沖液成份0.01M 磷酸鹽緩沖液（pH7.4，containing 0.05% Proclin-300，50% glycerol）
- 性狀液體
- 濃度1mg/mL
- 且適物種Mus musculus (Mouse，小鼠), Rattus norvegicus (Rat，大鼠), Cavia (Guinea pig ，豚鼠), Canis familiaris; Canine (Dog，犬), Bos taurus; Bovine (Cattle，牛), Chicken (Gallus，雞)
- 應(yīng)用WB; IHC; ICC/IF
如果抗體需用于流式細(xì)胞術(shù)，請參見流式抗體。 - 下載 英文說明書 中文說明書
- 規(guī)格 20μl100μl 200μl 1ml 10ml
- 價格 ¥ 630 ¥ 1470 ¥ 2100 ¥ 5250 ¥ 21000
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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FITC staining on IF; Samples: Human HepG2 cell; Primary Ab: 20μg/ml Mouse Anti-Human HSPA1A Antibody Second Ab: 1μg/ml FITC-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu18)
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DAB staining on IHC-P;
Samples: Human Breast cancer Tissue;
Primary Ab: 20μg/ml Mouse Anti-Human HSPA1A Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
DAB staining on IHC-P;
Samples: Human Prostate Tissue;
Primary Ab: 20μg/ml Mouse Anti-Human HSPA1A Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
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Figure. Western Blot; Sample: Recombinant HSPA1A, Human.
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Western Blot; Sample: Lane1: Mouse Cerebrum lysate; Lane2: Cavia Liver lysate; Lane3: Cavia Cerebrum lysate; Lane4: Canine Cerebrum lysate; Lane5: Bovine Liver lysate; Lane6: Bovine Cerebrum lysate<br/>Primary Ab: 1μg/ml Mouse Anti-Human HSPA1A Antibody<br/>Second Ab: 0.2μg/mL HRP-Linked Rabbit Anti-Mouse IgG Polyclonal Antibody<br/>(Catalog: SAA544Mu19)
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Western Blot; Sample: Lane1: Mouse Liver lysate; Lane2: Rat Liver lysate; Lane3: Canine Liver lysate<br/>Primary Ab: 1μg/ml Mouse Anti-Human HSPA1A Antibody<br/>Second Ab: 0.2μg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody<br/>(Catalog: SAA544Mu19)
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Western Blot; Sample: Lane1: Hela cell lysate; Lane2: Jurkat cell lysate; Lane3: Rat Cerebrum lysate; Lane4: Rat Uterus lysate
Primary Ab: 3μg/ml Mouse Anti-Human HSPA1A Antibody
Second Ab: 0.2μg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
Western Blot; Sample: Lane1: Bovine Liver lysate; Lane2: Bovine Cerebrum lysate; Lane3: HepG2 cell lysate; Lane4: Hela cell lysate<br/>Primary Ab: 0.2μg/ml Mouse Anti-Human HSPA1A Antibody<br/>Second Ab: 0.2μg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody<br/>(Catalog: SAA544Mu19)
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通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
該抗體是針對HSPA1A的小鼠單克隆抗體。在免疫組織化學(xué)染色和免疫印跡實(shí)驗(yàn)中能識別HSPA1A。
用法
Western blotting: 0.01-3μg/mL;
Immunohistochemistry: 5-20μg/mL;
Immunocytochemistry: 5-20μg/mL;
Optimal working dilutions must be determined by end user.
儲存
經(jīng)常使用則4°C保存。-20°C保存不超過兩年。避免反復(fù)凍融。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲，損失率低于5%。
贈品
增值服務(wù)
抗體標(biāo)記定制服務(wù)
Protein-A/G純化柱
細(xì)胞和組織染色液
抗體陽性對照品
組織/切片定制服務(wù)
磷酸化抗體定制服務(wù)
免疫印跡(WB)實(shí)驗(yàn)服務(wù)
免疫組織化學(xué)(IHC)實(shí)驗(yàn)服務(wù)
免疫細(xì)胞化學(xué)(ICC)實(shí)驗(yàn)服務(wù)
流式細(xì)胞術(shù)(FCM)實(shí)驗(yàn)服務(wù)
免疫沉淀(IP)實(shí)驗(yàn)服務(wù)
免疫熒光(IF)實(shí)驗(yàn)服務(wù)
緩沖液
DAB顯色試劑盒
SABC試劑盒
長臂生物素標(biāo)記試劑盒
實(shí)時熒光定量PCR實(shí)驗(yàn)服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| RPB081Hu02 | 70kDa熱休克蛋白1A(HSPA1A)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPB081Hu01 | 70kDa熱休克蛋白1A(HSPA1A)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| CAB081Hu01 | 抗70kDa熱休克蛋白1A(HSPA1A)多克隆抗體 | Loading Control of WB. |
| PAB081Hu01 | 70kDa熱休克蛋白1A(HSPA1A)多克隆抗體 | WB; IHC; ICC; IP; FCM |
| PAB081Hu02 | 70kDa熱休克蛋白1A(HSPA1A)多克隆抗體 | WB,IHC |
| FAB081Hu03 | 抗70kDa熱休克蛋白1A(HSPA1A)多克隆抗體 | FCM |
| MAB081Hu21 | 70kDa熱休克蛋白1A(HSPA1A)單克隆抗體 | WB; IHC; ICC/IF |
| MAB081Hu25 | 70kDa熱休克蛋白1A(HSPA1A)單克隆抗體 | WB |
| CAB081Hu22 | 抗70kDa熱休克蛋白1A(HSPA1A)單克隆抗體 | Loading Control of WB. |
| MAB081Hu22 | 70kDa熱休克蛋白1A(HSPA1A)單克隆抗體 | WB |
| FAB081Hu02 | 抗70kDa熱休克蛋白1A(HSPA1A)單克隆抗體 | Flow cytometry. |
| HEB081Hu | 70kDa熱休克蛋白1A(HSPA1A)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,高敏型) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SEB081Hu | 70kDa熱休克蛋白1A(HSPA1A)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMB081Hu | 70kDa熱休克蛋白1A(HSPA1A)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| KSB081Hu01 | 70kDa熱休克蛋白1A(HSPA1A)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) | Main materials for "Do It (ELISA Kit) Yourself". |
| CGB081Hu01 | 70kDa熱休克蛋白1A(HSPA1A)cDNA | PCR、蛋白表達(dá)等 |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| Journal of Neuroinflammation | Identification of potential biomarkers of gold nanoparticle toxicity in rat brains [PubMed: 22691312] |
| Mediators Inflamm.? | Local Inflammation in Fracture Hematoma: Results from a Combined Trauma Model in Pigs [Pubmed:Pmc4324980] |
| Eur J Pharmacol. | Differential modulatory effects of morphine on acute and chronic stress induced neurobehavioral and cellular markers in rats [Pubmed:24508522] |
| Pharmacol Rep. | Effect of propranolol on IL-10, visfatin, Hsp70, iNOS, TLR2, and survivin in amelioration of tumor progression and survival in Solid Ehrlich Carcinoma-bearing mice [Pubmed:25443743] |
| Advances in Medical Sciences | A similar pro/anti-inflammatory cytokine balance is present in the airways of competitive athletes and non-exercising asthmatics [pubmed:28822267] |
| Archives of Medical Research | Winter ambient training conditions are associated with increased bronchial hyperreactivity and with shifts in serum innate immunity proteins in young?… [Pubmed:29379533] |
| metabolic brain disease | Curcumin attenuates cytoplasmic/endoplasmic reticulum stress, apoptosis and cholinergic dysfunction in diabetic rat hippocampus [Pubmed: 32172517] |
| EXPERIMENTAL CELL RESEARCH | Antagomirs targeting miR-142–5p attenuate pilocarpine-induced status epilepticus in mice [Pubmed: 32439493] |
