肝素酶(HPSE)多克隆抗體
Polyclonal Antibody to Heparanase (HPSE)
HPA; HPR1; HPSE1; HSE1; Endo-glucoronidase;
- 編號(hào)PAA711Hu02
- 物種Homo sapiens (Human，人) 相同的名稱(chēng)，不同的物種。
- 來(lái)源多抗制備
- 宿主兔
- 效價(jià)-
- Ig類(lèi)型 IgG
- 純化方式抗原特異性親和純化
- 標(biāo)記物無(wú)標(biāo)記物
- 免疫原 RPA711Hu02-肝素酶(HPSE)重組蛋白
- 緩沖液成份0.01M 磷酸鹽緩沖液（pH7.4，containing 0.05% Proclin-300，50% glycerol）
- 性狀液體
- 濃度0.5mg/mL
- 且適物種Sus scrofa; Porcine (Pig，豬)
- 應(yīng)用WB,IHC,ICC/IF
如果抗體需用于流式細(xì)胞術(shù)，請(qǐng)參見(jiàn)流式抗體。 - 下載 英文說(shuō)明書(shū) 中文說(shuō)明書(shū)
- 規(guī)格 20μl100μl 200μl 1ml 10ml
- 價(jià)格 ¥ 569 ¥ 1327 ¥ 1896 ¥ 4740 ¥ 18960
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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DAB staining on IHC-P; Samples: Human Placenta Tissue; Primary Ab: 20μg/ml Rabbit Anti-Human HPA Antibody Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
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DAB staining on IHC-P;
Sample: Porcine Spleen Tissue
Primary Ab: 10μg/ml Rabbit Anti-Human HPSE Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
IHC圖
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DAB staining on IHC-P;
Sample: Human Spleen Tissue;
Primary Ab: 20μg/ml Rabbit Anti-Human HPA Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Secondary antibody only control: Used PBS instead of primary antibody, Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
FITC staining on IF;
Samples: Human HepG2 cell;
Primary Ab: 20μg/ml Rabbit Anti-Human HPA Antibody
Second Ab: 1.5μg/ml FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb18) -
FITC staining on IF;
Sample: HepG2 cell
Primary Ab: 20μg/ml Rabbit Anti-Human HPSE Antibody
Second Ab: 2μg/ml FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb11)
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Figure. Western Blot; Sample: Recombinant HPA, Human.
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Western Blot; Samples: Lane1: LO2 cell lysate; Lane2: HepG2 cell lysate; Lane3: Jurkat cell lysate;
Primary Ab: 0.1μg/ml Rabbit Anti-Human HPSE Antibody
Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Knockout Varification:
Lane 1: Wild-type HepG2 cell lysate;
Lane 2: HPA knockout HepG2 cell lysate;
Predicted MW: 43,53,55,61kd
Observed MW: 68kd
Primary Ab: 1μg/ml Rabbit Anti-Human HPA Antibody
Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
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通過(guò)ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
該抗體是針對(duì)HPSE的兔多克隆抗體。在免疫組織化學(xué)染色和免疫印跡實(shí)驗(yàn)中能識(shí)別HPSE。
用法
Western blotting: 0.01-2μg/mL;
Immunohistochemistry: 5-20μg/mL;
Immunofluorescence:5-20μg/mL;
Optimal working dilutions must be determined by end user.
儲(chǔ)存
經(jīng)常使用則4°C保存。-20°C保存不超過(guò)兩年。避免反復(fù)凍融。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定，具體方法如下：在37°C孵育48小時(shí)，沒(méi)有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲(chǔ)，損失率低于5%。
贈(zèng)品
增值服務(wù)
抗體標(biāo)記定制服務(wù)
Protein-A/G純化柱
細(xì)胞和組織染色液
抗體陽(yáng)性對(duì)照品
組織/切片定制服務(wù)
磷酸化抗體定制服務(wù)
免疫印跡(WB)實(shí)驗(yàn)服務(wù)
免疫組織化學(xué)(IHC)實(shí)驗(yàn)服務(wù)
免疫細(xì)胞化學(xué)(ICC)實(shí)驗(yàn)服務(wù)
流式細(xì)胞術(shù)(FCM)實(shí)驗(yàn)服務(wù)
免疫沉淀(IP)實(shí)驗(yàn)服務(wù)
免疫熒光(IF)實(shí)驗(yàn)服務(wù)
緩沖液
DAB顯色試劑盒
SABC試劑盒
長(zhǎng)臂生物素標(biāo)記試劑盒
實(shí)時(shí)熒光定量PCR實(shí)驗(yàn)服務(wù)
相關(guān)產(chǎn)品
| 編號(hào) | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| RPA711Hu04 | 肝素酶(HPSE)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA711Hu02 | 肝素酶(HPSE)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APA711Hu04 | 肝素酶(HPSE)活性蛋白 | Cell?culture;?Activity?Assays. |
| APA711Hu01 | 肝素酶(HPSE)活性蛋白 | Cell?culture;?Activity?Assays. |
| EPA711Hu61 | 肝素酶(HPSE)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APA711Hu61 | 肝素酶(HPSE)活性蛋白 | Cell?culture;?Activity?Assays. |
| RPA711Hu01 | 肝素酶(HPSE)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA711Hu03 | 肝素酶(HPSE)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| CPA711Hu21 | 肝素酶(HPSE)卵白蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
| PAA711Hu04 | 肝素酶(HPSE)多克隆抗體 | WB; IHC; ICC; IP. |
| PAA711Hu02 | 肝素酶(HPSE)多克隆抗體 | WB,IHC,ICC/IF |
| PAA711Hu01 | 肝素酶(HPSE)多克隆抗體 | WB; IHC; ICC; IP. |
| PAA711Hu03 | 肝素酶(HPSE)多克隆抗體 | WB; IHC; ICC; IP. |
| FAA711Hu01 | 抗肝素酶(HPSE)多克隆抗體 | Flow cytometry. |
| LAA711Hu71 | 肝素酶(HPSE)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| MAA711Hu23 | 肝素酶(HPSE)單克隆抗體 | WB |
| MAA711Hu22 | 肝素酶(HPSE)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA711Hu21 | 肝素酶(HPSE)單克隆抗體 | WB,ICC/IF |
| SEA711Hu | 肝素酶(HPSE)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| HEA711Hu | 肝素酶(HPSE)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,高敏型) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA711Hu | 肝素酶(HPSE)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| KSA711Hu01 | 肝素酶(HPSE)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| Plos one | PG545, a Heparan Sulfate Mimetic, Reduces Heparanase Expression In Vivo, Blocks Spontaneous Metastases and Enhances Overall Survival in the 4T1 Breast Carcinoma Model [Plosone: Source] |
| Journal of Medical Science | Assessment of heparanase and heparin-binding growth and angiogenesis factors in the uterine cavity ? uid in women with impaired reproduction [Nowinylekarskie:Source] |
| Int J Clin Exp Pathol | Unfractionated heparin attenuates intestinal injury in mouse model of sepsis by inhibiting heparanase [PubMed: 26191183] |
| Tumour Biology | PI-88 inhibits postoperative recurrence of hepatocellular carcinoma via disrupting the surge of heparanase after liver resection [PubMed: 26415733] |
| Journal of Medical Science | Assessment of heparanase and heparin-binding growth and angiogenesis factors in the uterine cavity fluid in women with impaired reproduction [article:84] |
| Renal Failure | Plasma heparanase is associated with blood glucose levels but not urinary microalbumin excretion in type 2 diabetic nephropathy at the early stage [pubmed:28994624] |
