肝素酶(HPSE)重組蛋白
Recombinant Heparanase (HPSE)
HPA; HPR1; HPSE1; HSE1; Endo-glucoronidase;
- 編號(hào)RPA711Hu02
- 物種Homo sapiens (Human,人) 相同的名稱,不同的物種。
- 來(lái)源原核表達(dá)
- 宿主E.coli
- 內(nèi)毒素水平<1.0EU/µg(LAL法測(cè)定)
- 亞細(xì)胞定位細(xì)胞核, 分泌, 溶酶體
- 預(yù)測(cè)分子量10.5kDa
- 實(shí)際分子量14kDa(差異分析請(qǐng)參閱說(shuō)明書)
- 片段與標(biāo)簽Gln34~Arg115 with N-terminal His Tag
- 緩沖液成份20mM Tris, 150mM NaCl緩沖液(pH8.0, 含有1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose和Proclin300)
- 性狀凍干粉
- 純度> 95%
- 等電點(diǎn)6.1
-
應(yīng)用
Positive Control; Immunogen; SDS-PAGE; WB.
如果需要有生物活性的蛋白,請(qǐng)參見(jiàn)活性蛋白。 - 下載 英文說(shuō)明書 中文說(shuō)明書
- 規(guī)格 10μg50μg 200μg 1mg 5mg
- 價(jià)格 ¥ 998 ¥ 2496 ¥ 4992 ¥ 14976 ¥ 37440
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序列
用法
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
儲(chǔ)存
避免反復(fù)凍融。2-8°C不超過(guò)一個(gè)月,-80°C不超過(guò)12個(gè)月。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定,具體方法如下:在37°C孵育48小時(shí),沒(méi)有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi),在適當(dāng)?shù)臈l件下存儲(chǔ),損失率低于5%。
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相關(guān)產(chǎn)品
編號(hào) | 適用物種:Homo sapiens (Human,人) | 應(yīng)用(僅供研究使用,不用于臨床診斷!) |
RPA711Hu04 | 肝素酶(HPSE)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
RPA711Hu02 | 肝素酶(HPSE)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
APA711Hu04 | 肝素酶(HPSE)活性蛋白 | Cell?culture;?Activity?Assays. |
APA711Hu01 | 肝素酶(HPSE)活性蛋白 | Cell?culture;?Activity?Assays. |
EPA711Hu61 | 肝素酶(HPSE)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
APA711Hu61 | 肝素酶(HPSE)活性蛋白 | Cell?culture;?Activity?Assays. |
RPA711Hu01 | 肝素酶(HPSE)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
RPA711Hu03 | 肝素酶(HPSE)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
CPA711Hu21 | 肝素酶(HPSE)卵白蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
PAA711Hu04 | 肝素酶(HPSE)多克隆抗體 | WB; IHC; ICC; IP. |
PAA711Hu02 | 肝素酶(HPSE)多克隆抗體 | WB,IHC,ICC/IF |
PAA711Hu01 | 肝素酶(HPSE)多克隆抗體 | WB; IHC; ICC; IP. |
PAA711Hu03 | 肝素酶(HPSE)多克隆抗體 | WB; IHC; ICC; IP. |
FAA711Hu01 | 抗肝素酶(HPSE)多克隆抗體 | Flow cytometry. |
LAA711Hu71 | 肝素酶(HPSE)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
MAA711Hu23 | 肝素酶(HPSE)單克隆抗體 | WB |
MAA711Hu22 | 肝素酶(HPSE)單克隆抗體 | WB; IHC; ICC; IP. |
MAA711Hu21 | 肝素酶(HPSE)單克隆抗體 | WB,ICC/IF |
SEA711Hu | 肝素酶(HPSE)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
HEA711Hu | 肝素酶(HPSE)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,高敏型) | Enzyme-linked immunosorbent assay for Antigen Detection. |
LMA711Hu | 肝素酶(HPSE)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
KSA711Hu01 | 肝素酶(HPSE)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻(xiàn)
雜志 | 參考文獻(xiàn) |
Plos one | PG545, a Heparan Sulfate Mimetic, Reduces Heparanase Expression In Vivo, Blocks Spontaneous Metastases and Enhances Overall Survival in the 4T1 Breast Carcinoma Model [Plosone: Source] |
Journal of Medical Science | Assessment of heparanase and heparin-binding growth and angiogenesis factors in the uterine cavity ? uid in women with impaired reproduction [Nowinylekarskie:Source] |
Int J Clin Exp Pathol | Unfractionated heparin attenuates intestinal injury in mouse model of sepsis by inhibiting heparanase [PubMed: 26191183] |
Tumour Biology | PI-88 inhibits postoperative recurrence of hepatocellular carcinoma via disrupting the surge of heparanase after liver resection [PubMed: 26415733] |
Journal of Medical Science | Assessment of heparanase and heparin-binding growth and angiogenesis factors in the uterine cavity fluid in women with impaired reproduction [article:84] |
Renal Failure | Plasma heparanase is associated with blood glucose levels but not urinary microalbumin excretion in type 2 diabetic nephropathy at the early stage [pubmed:28994624] |
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