肝素酶(HPSE)重組蛋白
Recombinant Heparanase (HPSE)
HPA; HPR1; HPSE1; HSE1; Endo-glucoronidase;
- 編號RPA711Hu01
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 來源原核表達
- 宿主E.coli
- 內(nèi)毒素水平<1.0EU/µg(LAL法測定)
- 亞細(xì)胞定位細(xì)胞核, 分泌
- 預(yù)測分子量17.6kDa
- 實際分子量20kDa(差異分析請參閱說明書)
- 片段與標(biāo)簽Pro400~Ile543 with N-terminal His Tag
- 緩沖液成份20mM Tris, 150mM NaCl緩沖液（pH8.0, 含有1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose和Proclin300）
- 性狀凍干粉
- 純度> 97%
- 等電點9.9
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應(yīng)用
Positive Control; Immunogen; SDS-PAGE; WB.
如果需要有生物活性的蛋白，請參見活性蛋白。 - 下載 英文說明書 中文說明書
- 規(guī)格 10μg50μg 200μg 1mg 5mg
- 價格 ¥ 998 ¥ 2496 ¥ 4992 ¥ 14976 ¥ 37440
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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SDS-PAGE圖
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通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
序列
用法
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
儲存
避免反復(fù)凍融。2-8°C不超過一個月，-80°C不超過12個月。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲，損失率低于5%。
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| RPA711Hu04 | 肝素酶(HPSE)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA711Hu02 | 肝素酶(HPSE)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APA711Hu04 | 肝素酶(HPSE)活性蛋白 | Cell?culture;?Activity?Assays. |
| APA711Hu01 | 肝素酶(HPSE)活性蛋白 | Cell?culture;?Activity?Assays. |
| EPA711Hu61 | 肝素酶(HPSE)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APA711Hu61 | 肝素酶(HPSE)活性蛋白 | Cell?culture;?Activity?Assays. |
| RPA711Hu01 | 肝素酶(HPSE)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA711Hu03 | 肝素酶(HPSE)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| CPA711Hu21 | 肝素酶(HPSE)卵白蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
| PAA711Hu04 | 肝素酶(HPSE)多克隆抗體 | WB; IHC; ICC; IP. |
| PAA711Hu02 | 肝素酶(HPSE)多克隆抗體 | WB,IHC,ICC/IF |
| PAA711Hu01 | 肝素酶(HPSE)多克隆抗體 | WB; IHC; ICC; IP. |
| PAA711Hu03 | 肝素酶(HPSE)多克隆抗體 | WB; IHC; ICC; IP. |
| FAA711Hu01 | 抗肝素酶(HPSE)多克隆抗體 | Flow cytometry. |
| LAA711Hu71 | 肝素酶(HPSE)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| MAA711Hu23 | 肝素酶(HPSE)單克隆抗體 | WB |
| MAA711Hu22 | 肝素酶(HPSE)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA711Hu21 | 肝素酶(HPSE)單克隆抗體 | WB,ICC/IF |
| SEA711Hu | 肝素酶(HPSE)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| HEA711Hu | 肝素酶(HPSE)檢測試劑盒(酶聯(lián)免疫吸附試驗法,高敏型) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA711Hu | 肝素酶(HPSE)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| KSA711Hu01 | 肝素酶(HPSE)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻
| 雜志 | 參考文獻 |
| Plos one | PG545, a Heparan Sulfate Mimetic, Reduces Heparanase Expression In Vivo, Blocks Spontaneous Metastases and Enhances Overall Survival in the 4T1 Breast Carcinoma Model [Plosone: Source] |
| Journal of Medical Science | Assessment of heparanase and heparin-binding growth and angiogenesis factors in the uterine cavity ? uid in women with impaired reproduction [Nowinylekarskie:Source] |
| Int J Clin Exp Pathol | Unfractionated heparin attenuates intestinal injury in mouse model of sepsis by inhibiting heparanase [PubMed: 26191183] |
| Tumour Biology | PI-88 inhibits postoperative recurrence of hepatocellular carcinoma via disrupting the surge of heparanase after liver resection [PubMed: 26415733] |
| Journal of Medical Science | Assessment of heparanase and heparin-binding growth and angiogenesis factors in the uterine cavity fluid in women with impaired reproduction [article:84] |
| Renal Failure | Plasma heparanase is associated with blood glucose levels but not urinary microalbumin excretion in type 2 diabetic nephropathy at the early stage [pubmed:28994624] |
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