G蛋白偶聯(lián)雌激素受體1(GPER)檢測試劑盒(酶聯(lián)免疫吸附試驗法)
ELISA Kit for G Protein Coupled Estrogen Receptor 1 (GPER)
GPR30; CMKRL2; DRY12; FEG-1; GPCR-Br; LERGU; LyGPR; Membrane estrogen receptor; Chemoattractant receptor-like 2; Flow-induced endothelial G-protein coupled receptor 1
- 編號SEG045Hu
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 實驗方法雙抗夾心
- 反應時長3h
- 檢測范圍0.312-20ng/mL
- 靈敏度最小可檢測劑量小于等于0.125ng/mL.
- 樣本類型serum, plasma, tissue homogenates and other biological fluids
- 下載 英文說明書 中文說明書
- 規(guī)格 48T96T 96T*5 96T*10 96T*100
- 價格 ¥ 2940 ¥ 4200 ¥ 18900 ¥ 35700 ¥ 294000
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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實驗結(jié)果圖
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標準曲線圖
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通過ISO 9001、ISO 13485質(zhì)量體系認證
特異性
本試劑盒用于檢測G蛋白偶聯(lián)雌激素受體1(GPER)，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應。
由于受到技術(shù)及樣本來源的限制，不可能完成對所有相關(guān)或相似物質(zhì)交叉反應檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應。
回收率
分別于定值血清及血漿樣本中加入一定量的G蛋白偶聯(lián)雌激素受體1(GPER)（加標樣品），重復測定并計算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 78-99 | 88 |
| EDTA plasma(n=5) | 95-102 | 98 |
| heparin plasma(n=5) | 88-96 | 93 |
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定，每個樣本使用同一試劑盒重復測定8次，分別計算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的G蛋白偶聯(lián)雌激素受體1(GPER)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中G蛋白偶聯(lián)雌激素受體1(GPER)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 93-101% | 95-103% | 92-99% | 80-102% |
| EDTA plasma(n=5) | 84-103% | 96-103% | 93-101% | 90-98% |
| heparin plasma(n=5) | 93-101% | 86-97% | 92-99% | 82-99% |
穩(wěn)定性
經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內(nèi)溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。
實驗流程
1. 實驗前標準品、試劑及樣本的準備；
2. 加樣（標準品及樣本）100µL，37°C孵育1小時；
3. 吸棄，加檢測溶液A100µL，37°C孵育1小時；
4. 洗板3次；
5. 加檢測溶液B100µL，37°C孵育30分鐘；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分鐘；
8. 加終止液50µL，立即450nm讀數(shù)。
實驗原理
將G蛋白偶聯(lián)雌激素受體1(GPER)抗體包被于96孔微孔板中，制成固相載體，向微孔中分別加入標準品或標本，其中的G蛋白偶聯(lián)雌激素受體1(GPER)與連接于固相載體上的抗體結(jié)合，然后加入生物素化的G蛋白偶聯(lián)雌激素受體1(GPER)抗體，將未結(jié)合的生物素化抗體洗凈后，加入HRP標記的親和素，再次徹底洗滌后加入TMB底物顯色。TMB在過氧化物酶的催化下轉(zhuǎn)化成藍色，并在酸的作用下轉(zhuǎn)化成最終的黃色。顏色的深淺和樣品中的G蛋白偶聯(lián)雌激素受體1(GPER)呈正相關(guān)。用酶標儀在450nm波長下測定吸光度(O.D.值)，計算樣品濃度。
增值服務
相關(guān)產(chǎn)品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應用(僅供研究使用，不用于臨床診斷！) |
| RPG045Hu01 | G蛋白偶聯(lián)雌激素受體1(GPER)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAG045Hu01 | G蛋白偶聯(lián)雌激素受體1(GPER)多克隆抗體 | WB; IHC; ICC; IP. |
| MAG045Hu21 | G蛋白偶聯(lián)雌激素受體1(GPER)單克隆抗體 | WB; IHC; ICC; IP. |
| SEG045Hu | G蛋白偶聯(lián)雌激素受體1(GPER)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMG045Hu | G蛋白偶聯(lián)雌激素受體1(GPER)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻
| 雜志 | 參考文獻 |
| The Journal of Steroid Biochemistry and Molecular Biology | Neuroprotective action of raloxifene against hypoxia-induced damage in mouse hippocampal cells depends on ERα but not ERβ or GPR30 signalling [Pubmed:24846829] |
| Molecular and Cellular Endocrinology | Isomer-nonspecific action of dichlorodiphenyltrichloroethane on aryl hydrocarbon receptor and G-protein-coupled receptor 30 intracellular signaling in apoptotic neuronal cells [Pubmed:24859647] |
| J Steroid Biochem Mol Biol | Neuroprotective action of raloxifene against hypoxia-induced damage in mouse hippocampal cells depends on ERα but not ERβ or GPR32 signalling [PubMed: 24846831] |
| Journal of Environmental Sciences | Bisphenol A exposure alters release of immune and developmental modulators and expression of estrogen receptors in human fetal lung fibroblasts [science:S1001074216300924] |
| Psychiatry research | Serum levels of G protein-coupled estrogen receptor 1 (GPER1) in drug-naive patients with generalized anxiety disorder. [pubmed:27512921] |
| Clinical Psychopharmacology Neuroscience. | Increased Serum G Protein-coupled Estrogen Receptor 1 Levels and Its Diagnostic Value in Drug Na?ve Patients with Major Depressive Disorder [pubmed:29073745] |
| Psychiatry?Research | Decreased levels of G protein-coupled estrogen receptor in children with autism spectrum disorders. [pubmed:28734238] |
| Neuropsychiatric disease and treatment | serum levels of gPer-1 in euthymic bipolar patients [Pubmed:29618927] |
| European archives of oto-rhino-laryngology | An evaluation of G-protein coupled membrane estrogen receptor-1 level in stuttering [Pubmed:29299745] |
| Bosnian Journal of?Basic?Medical?Sciences | Evaluation of estrogen and G protein-coupled estrogen receptor 1 (GPER) levels in drug-na?ve patients with attention deficit hyperactivity disorder (ADHD) [Pubmed:29659348] |
| Carleton University Research Virtual Environment | In Vitro Study of Mechanisms Underlying the Developmental Effects of Bisphenol A Using Human Fetal Lung Fibroblasts [] |
| Am J Otolaryngol | GPER-1 and sex-hormone levels in patients with otosclerosis [Pubmed: 32144019] |
| Andrologia. | Relationship between the G protein–coupled oestrogen receptor and spermatogenesis, and its correlation with male infertility [Pubmed: 32776559] |
| Journal of Radiation Research and Applied Sciences | GPER-1 in chronic hepatitis B [] |
| FASEB J | Endocrine disruption in Crohn's disease: Bisphenol A enhances systemic inflammatory response in patients with gut barrier translocation of dysbiotic microbiota??- [34085740] |
| Comparing the Impact of Serum GPER-1 and Oxidant/Antioxidant Levels on Retinopathy in Diabetic Patients and Healthy Individuals: A Pilot Study [] | |
| Horm Mol Biol Clin Investig | The change pattern in serum G protein-coupled estrogen receptor-1 (GPER1) levels during pregnancy with and without gestational diabetes mellitus [34787384] |
