脂多糖結(jié)合蛋白(LBP)檢測試劑盒(酶聯(lián)免疫吸附試驗法)
ELISA Kit for Lipopolysaccharide Binding Protein (LBP)
LPS-Binding Protein
- 編號SEB406Bo
- 物種Bos taurus; Bovine (Cattle，牛) 相同的名稱，不同的物種。
- 實驗方法雙抗夾心
- 反應(yīng)時長3h
- 檢測范圍0.625-40ng/mL
- 靈敏度最小可檢測劑量小于等于0.275ng/mL.
- 樣本類型serum, plasma and other biological fluids
- 下載 英文說明書 中文說明書
- 規(guī)格 48T96T 96T*5 96T*10 96T*100
- 價格 ¥ 3352 ¥ 4788 ¥ 21546 ¥ 40698 ¥ 335160
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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實驗結(jié)果圖
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標(biāo)準(zhǔn)曲線圖
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通過ISO 9001、ISO 13485質(zhì)量體系認證
特異性
本試劑盒用于檢測脂多糖結(jié)合蛋白(LBP)，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來源的限制，不可能完成對所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應(yīng)。
回收率
分別于定值血清及血漿樣本中加入一定量的脂多糖結(jié)合蛋白(LBP)（加標(biāo)樣品），重復(fù)測定并計算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 86-104 | 94 |
| EDTA plasma(n=5) | 88-103 | 93 |
| heparin plasma(n=5) | 85-94 | 89 |
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定，每個樣本使用同一試劑盒重復(fù)測定8次，分別計算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的脂多糖結(jié)合蛋白(LBP)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中脂多糖結(jié)合蛋白(LBP)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 93-101% | 84-91% | 83-101% | 90-98% |
| EDTA plasma(n=5) | 78-99% | 80-93% | 95-102% | 79-93% |
| heparin plasma(n=5) | 81-95% | 92-101% | 83-97% | 83-105% |
穩(wěn)定性
經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內(nèi)溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。
實驗流程
1. 實驗前標(biāo)準(zhǔn)品、試劑及樣本的準(zhǔn)備；
2. 加樣（標(biāo)準(zhǔn)品及樣本）100µL，37°C孵育1小時；
3. 吸棄，加檢測溶液A100µL，37°C孵育1小時；
4. 洗板3次；
5. 加檢測溶液B100µL，37°C孵育30分鐘；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分鐘；
8. 加終止液50µL，立即450nm讀數(shù)。
實驗原理
將脂多糖結(jié)合蛋白(LBP)抗體包被于96孔微孔板中，制成固相載體，向微孔中分別加入標(biāo)準(zhǔn)品或標(biāo)本，其中的脂多糖結(jié)合蛋白(LBP)與連接于固相載體上的抗體結(jié)合，然后加入生物素化的脂多糖結(jié)合蛋白(LBP)抗體，將未結(jié)合的生物素化抗體洗凈后，加入HRP標(biāo)記的親和素，再次徹底洗滌后加入TMB底物顯色。TMB在過氧化物酶的催化下轉(zhuǎn)化成藍色，并在酸的作用下轉(zhuǎn)化成最終的黃色。顏色的深淺和樣品中的脂多糖結(jié)合蛋白(LBP)呈正相關(guān)。用酶標(biāo)儀在450nm波長下測定吸光度(O.D.值)，計算樣品濃度。
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Bos taurus; Bovine (Cattle，牛) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| APB406Bo01 | 脂多糖結(jié)合蛋白(LBP)活性蛋白 | Cell?culture;?Activity?Assays. |
| RPB406Bo01 | 脂多糖結(jié)合蛋白(LBP)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAB406Bo01 | 脂多糖結(jié)合蛋白(LBP)多克隆抗體 | WB; IHC; ICC; IP. |
| LAB406Bo71 | 脂多糖結(jié)合蛋白(LBP)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| MAB406Bo21 | 脂多糖結(jié)合蛋白(LBP)單克隆抗體 | WB; IHC; ICC; IP. |
| SEB406Bo | 脂多糖結(jié)合蛋白(LBP)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMB406Bo | 脂多糖結(jié)合蛋白(LBP)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻
| 雜志 | 參考文獻 |
| 37 | A Marker of Endotoxemia Is Associated With Obesity and Related Metabolic Disorders in Apparently Healthy Chinese [Diabetes: source] |
| AIDS | Oral serum-derived bovine immunoglobulin improves duodenal immune reconstitution and absorption function in patients with HIV enteropathy [PubMed: PMC3754419] |
| 31 | Gut microbiome composition is linked to whole grain-induced immunological improvements [Pubmed: 23038174] |
| The Veterinary Journal | High ambient temperature alleviates the inflammatory response and growth depression in pigs challenged with Escherichia coli lipopolysaccharide [Pubmed: 24792207] |
| Perfusion | The effect of normovolemic modified ultrafiltration on inflammatory mediators, endotoxins, terminal complement complexes and clinical outcome in high-risk cardiac surgery patients [Pubmed: 23429100] |
| FEMS Microbiology Ecology | A gut microbiota-targeted dietary intervention for amelioration of chronic inflammation underlying metabolic syndrome. [Pubmed: 24117923] |
| Digestive diseases and sciences | Frequency and Severity of Cirrhotic Cardiomyopathy and Its Possible Relationship with Bacterial Endotoxemia [Pubmed: 23907333] |
| Atherosclerosis | Circulating pathogen associated molecular pattern – Binding proteins and High Mobility Group Box protein 1 in nascent metabolic syndrome: Implications for cellular Toll-like receptor activity [Pubmed:25063948] |
| BMC Complementary and Alternative Medicine | Identification of plasma protein markers common to patients with malignant tumour and Abnormal Savda in Uighur medicine: a prospective clinical study [Pubmed:25652121] |
| Atherosclerosis | Increased adipose tissue secretion of Fetuin-A, lipopolysaccharide-binding protein and high-mobility group box protein 1 in metabolic syndrome [PubMed: 25978344] |
| Annals of Gastroenterology | Systemic levels of human β-defensin 1 are elevated in patients with cirrhosis [PubMed: 26751578] |
| Food Chem. | Comparative proteomics of milk fat globule membrane in goat colostrum and mature milk [Pubmed:27173528] |
| Liver International | High serum lipopolysaccharide binding protein is associated with increased mortality in patients with decompensated cirrhosis [pubmed:27712029] |
| Journal of Functional Foods | Modulation of gut microbiota and gut-generated metabolites by bitter melon results in improvement in the metabolic status in high fat diet-induced obese rats [10.1016/j.jff.2017.12.050] |
| Scientific Reports | Adamdec1, Ednrb and Ptgs1/Cox1, inflammation genes upregulated in the intestinal mucosa of obese rats, are downregulated by three probiotic strains. [pubmed:28512356] |
| Journal of ethnopharmacology | A standardized extract of the fruit of Hovenia dulcis alleviated alcohol-induced hangover in healthy subjects with heterozygous ALDH2: A randomized, controlled, crossover trial. [pubmed:28750942] |
| Journal of?Allergy?and Clinical Immunology | On a Western diet, APOE ?4 is associated with low innate immune sensing, but not APOE ?3 [Pubmed:29928926] |
| Journal of?Animal?Sciences | Influence of yeast culture and feed antibiotics on ruminal fermentation and site and extent of digestion in beef heifers fed high grain rations [Pubmed:30060086] |
| Journal?of?Animal?Science | Using ruminally protected and nonprotected active dried yeast as alternatives to antibiotics in finishing beef steers: growth performance, carcass traits, blood?… [Pubmed: 30184125] |
| BMC?Complementary and Alternative?Medicine | Evaluation of the effect of Lactobacillus reuteri V3401 on biomarkers of inflammation, cardiovascular risk and liver steatosis in obese adults with metabolic?… [Pubmed: 30453950] |
| Cell Communication and Signaling | Oridonin protects LPS-induced acute lung injury by modulating Nrf2-mediated oxidative stress and Nrf2-independent NLRP3 and NF-κB pathways [] |
| Nutrients | High-dose Glycerol Monolaurate Up-Regulated Beneficial Indigenous Microbiota without Inducing Metabolic Dysfunction and Systemic Inflammation: New Insights?… [] |
| Journal of Animal Science | Ruminally protected and unprotected Saccharomyces cerevisiae fermentation products as alternatives to antibiotics in finishing beef steers [Pubmed: 31410465] |
| Nutrients | Lactobacillus reuteri V3401 Reduces Inflammatory Biomarkers and Modifies the Gastrointestinal Microbiome in Adults with Metabolic Syndrome: The PROSIR Study [Pubmed: 31370223] |
| journal of veterinary pharmacology and therapeutics | Effects of sinomenine in LPS‐associated diseases are related to inhibition of LBP, Mac‐1, and L‐selectin levels [Pubmed: 31490576] |
| Amyloid | Needle-shaped amyloid deposition in rat mammary gland: evidence of a novel amyloid fibril protein [Pubmed: 31615282] |
| Food Research International | Ethanol Extract of Propolis Prevents High-Fat Diet-Induced Insulin Resistance and Obesity in Association with Modulation of Gut Microbiota in Mice [Pubmed: 32156386] |
| Journal of Translational Medicine | Gut microbiota from coronary artery disease patients contributes to vascular dysfunction in mice by regulating bile acid metabolism and immune activation [Pubmed: 33036625] |
| Communications Biology | Oxidation of fish oil exacerbates alcoholic liver disease by enhancing intestinal dysbiosis in mice [Pubmed: 32879433] |
| MOLECULAR NUTRITION & FOOD RESEARCH | Antibiotic Treatment Reduces the Health Benefits of Soy Protein [Pubmed: 32729948] |
| JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | Highly Branched RG-I Domain Enrichment are Indispensable for Pectin Mitigating Against High-Fat Diet-Induced Obesity [Pubmed: 32633953] |
| fish & shellfish immunology | Molecular characterization and expression analysis of chemokine (CXCL12) from Nile tilapia (Oreochromis niloticus) [Pubmed: 32540504] |
| Scientific Reports | Tandem Mass Tag (TMT)-based quantitative proteomics reveals potential targets associated with onset of Sub-clinical Mastitis in cows [Pubmed: 32518370] |
| experimental eye research | Corneal epithelial injury-induced norepinephrine promotes Pseudomonas aeruginosa keratitis [Pubmed: 32376471] |
| Frontiers in Microbiology | Naringin Attenuates High Fat Diet Induced Non-alcoholic Fatty Liver Disease and Gut Bacterial Dysbiosis in Mice [] |
| Journal of Burn Care & Research | Umbilical cord mesenchymal stem cells (hUCMSCs) for inflammatory regulation after excision and grafting of severe burn wounds in rats [33313794] |
| JOURNAL OF ANIMAL SCIENCE | Processing Index of Barley Grain and Dietary Undigested Neutral Detergent Fiber Concentration Affected Chewing Behavior, Ruminal pH and Total Tract Nutrient??- [33474564] |
| journal of agricultural and food chemistry | Influence of a Biotechnologically Produced Oyster Mushroom (Pleurotus sajor-caju) on the Gut Microbiota and Microbial Metabolites in Obese Zucker Rats [33497213] |
| JOURNAL OF HAZARDOUS MATERIALS | Melatonin alleviates Ochratoxin A-induced liver inflammation involved intestinal microbiota homeostasis and intestinal microbiota-independent manner [33582472] |
| Doctoral Thesis | Studies on Mastitis Caused by Translocated-Bacterial Components in Ruminants [] |
| Arthritis Res Ther | Monocyte transcriptomes from patients with axial spondyloarthritis reveal dysregulated monocytopoiesis and a distinct inflammatory imprint [34560894] |
| Microorganisms | Composition and Functions of the Gut Microbiome in Pediatric Obesity: Relationships with Markers of Insulin Resistance [34361925] |
| Antioxidants | The Prebiotic Potential of Geraniin and Geraniin-Enriched Extract against High-Fat-Diet-Induced Metabolic Syndrome in Sprague Dawley Rats [Pubmed:35453317] |
| Microbiome | Elucidating the role of the gut microbiota in the physiological effects of dietary fiber [Pubmed:35562794] |
| Bioscience of Microbiota Food and Health | Local free fatty acids trigger the expression of lipopolysaccharide-binding protein in murine white adipose tissue [Pubmed:35433160] |
| Siberian Medical Review | Сorrelation between the level of 25 (OH) D3 in blood, the content of antimicrobial peptides in oral fluid and dental caries intensity in young individuals [] |
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