脂多糖結(jié)合蛋白(LBP)活性蛋白
Active Lipopolysaccharide Binding Protein (LBP)
LPS-Binding Protein
- 編號APB406Bo01
- 物種Bos taurus; Bovine (Cattle，牛) 相同的名稱，不同的物種。
- 緩沖液成份PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
- 性狀凍干粉
- 純度> 95%
- 等電點6.8
- 應(yīng)用Cell?culture;?Activity?Assays.
- 下載 英文說明書 中文說明書
- 規(guī)格 10μg50μg 200μg 1mg 5mg
- 價格 ¥ 1920 ¥ 4800 ¥ 9600 ¥ 28800 ¥ 72000
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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Figure. SDS-PAGE
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通過ISO 9001、ISO 13485質(zhì)量體系認證
活性實驗
Lipopolysaccharide Binding Protein (LBP) is a soluble acute-phase protein that binds to bacterial lipopolysaccharide (or LPS) to elicit immune responses by presenting the LPS to important cell surface pattern recognition receptors called CD14 and TLR4. This protein is part of a family of structurally and functionally related proteins, including BPI, plasma cholesteryl ester transfer protein (CETP), and phospholipid transfer protein (PLTP). It has been reported that LBP can enhance LPS-stimulated IL-8 secretion by THP-1 cells. To test the bioactivity of recombinant bovine LBP, THP-1 cells were seeded into 24-well plate at a density of 1x106 cells/mL including 1 ug/mL LPS, and treated with certain concentrations (0.078125 ug/mL-1.25 ug/mL) of rbLBP for 24h and IL-8 levels in the cell supernatant were determined by ELISA. IL-8 levels in the cell supernatant of THP-1 cells increased significantly after stimulated with recombinant bovine LBP have shown in Figure 1.
用法
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
儲存
避免反復(fù)凍融。2-8°C不超過一個月，-80°C不超過12個月。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當?shù)臈l件下存儲，損失率低于5%。
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Bos taurus; Bovine (Cattle，牛) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| APB406Bo01 | 脂多糖結(jié)合蛋白(LBP)活性蛋白 | Cell?culture;?Activity?Assays. |
| RPB406Bo01 | 脂多糖結(jié)合蛋白(LBP)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAB406Bo01 | 脂多糖結(jié)合蛋白(LBP)多克隆抗體 | WB; IHC; ICC; IP. |
| LAB406Bo71 | 脂多糖結(jié)合蛋白(LBP)多克隆抗體(生物素標記) | WB; IHC; ICC. |
| MAB406Bo21 | 脂多糖結(jié)合蛋白(LBP)單克隆抗體 | WB; IHC; ICC; IP. |
| SEB406Bo | 脂多糖結(jié)合蛋白(LBP)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMB406Bo | 脂多糖結(jié)合蛋白(LBP)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻
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