肌動(dòng)蛋白β(ACTB)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法)
Multiplex Assay Kit for Beta Actin (ACTB) ,etc. by FLIA (Flow Luminescence Immunoassay)
Actin Beta; ACT-B; PS1TP5BP1; Actin, cytoplasmic 1; Actin, cytoplasmic 1, N-terminally processed
(注:?jiǎn)未位鞙y(cè)多因子不超過(guò)8個(gè)指標(biāo) )
- 編號(hào)LMB340Mi
- 物種Homo sapiens (Human,人), Mus musculus (Mouse,小鼠), Rattus norvegicus (Rat,大鼠) 相同的名稱(chēng),不同的物種。
- 實(shí)驗(yàn)方法雙抗夾心
- 反應(yīng)時(shí)長(zhǎng)3.5h
- 檢測(cè)范圍0.1-100ng/mL
- 靈敏度最小可檢測(cè)劑量小于等于0.033 ng/mL.
- 樣本類(lèi)型Tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
- 下載 英文說(shuō)明書(shū) 中文說(shuō)明書(shū)
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特異性
本試劑盒對(duì)檢測(cè)肌動(dòng)蛋白β(ACTB)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法)具有較高的靈敏度。
經(jīng)檢測(cè)人、小鼠、大鼠之間有100%的交叉反映。
回收率
分別于定值血清及血漿樣本中加入一定量的肌動(dòng)蛋白β(ACTB)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法)(加標(biāo)樣品),重復(fù)測(cè)定并計(jì)算其均值,回收率為測(cè)定值與理論值的比率。
樣本 | 回收率范圍(%) | 平均回收率(%) |
serum(n=5) | 98-105 | 102 |
EDTA plasma(n=5) | 93-105 | 101 |
heparin plasma(n=5) | 96-105 | 101 |
sodium citrate plasma(n=5) | 96-103 | 99 |
精密度
精密度用樣品測(cè)定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差:取同批次試劑盒對(duì)低、中、高值定值樣本進(jìn)行定量檢測(cè),每份樣本連續(xù)測(cè)定20 次,分別計(jì)算不同濃度樣本的平均值及SD值。
批間差:選取3個(gè)不同批次的試劑盒分別對(duì)低、中、高值定值樣本進(jìn)行定量測(cè)定,每個(gè)樣本使用同一試劑盒重復(fù)測(cè)定8次,分別計(jì)算不同濃度樣本的平均值及SD值。
批內(nèi)差: CV<10%
批間差: CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的肌動(dòng)蛋白β(ACTB)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法),并倍比稀釋成1:2,1:4,1:8,1:16的待測(cè)樣本,線性范圍即為稀釋后樣本中肌動(dòng)蛋白β(ACTB)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法)含量的測(cè)定值與理論值的比率。
樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
serum(n=5) | 90-101% | 80-94% | 97-105% | 85-95% |
EDTA plasma(n=5) | 97-105% | 87-95% | 92-101% | 78-103% |
heparin plasma(n=5) | 85-93% | 81-92% | 95-103% | 89-96% |
sodium citrate plasma(n=5) | 94-102% | 81-93% | 91-99% | 81-93% |
穩(wěn)定性
經(jīng)測(cè)定,試劑盒在有效期內(nèi)按推薦溫度保存,其活性降低率小于5%。
為減小外部因素對(duì)試劑盒破壞前后檢測(cè)值的影響,實(shí)驗(yàn)室的環(huán)境條件需盡量保持一致,尤其是實(shí)驗(yàn)室內(nèi)溫度、濕度及溫育條件。其次由同一實(shí)驗(yàn)員來(lái)進(jìn)行操作可減少人為誤差。
實(shí)驗(yàn)流程
1. 實(shí)驗(yàn)前標(biāo)準(zhǔn)品、試劑及樣本準(zhǔn)備;
2. 加樣(標(biāo)準(zhǔn)品、樣本、磁珠)標(biāo)準(zhǔn)品或樣本100μL及磁珠10μL,
37°C酶標(biāo)板振蕩器孵育90分鐘;
3. 磁吸甩干,加檢測(cè)溶液A100μL,37°C酶標(biāo)板振蕩器孵育60分鐘;
4. 磁吸洗板3次;
5. 加檢測(cè)溶液B100μL,37°C振動(dòng)孵育30分鐘;
6. 磁吸洗板3次;
7. 加鞘液100μL,旋渦震蕩2分鐘后讀數(shù)。
實(shí)驗(yàn)原理
將肌動(dòng)蛋白β(ACTB)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法)抗體包被于磁珠,制成固相載體,向微孔中分別加入標(biāo)準(zhǔn)品或標(biāo)本以及磁珠,其中的肌動(dòng)蛋白β(ACTB)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法)與連接于固相載體上的抗體結(jié)合,然后加入生物素化的肌動(dòng)蛋白β(ACTB)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法)抗體,將未結(jié)合的生物素化抗體洗凈后,加入PE標(biāo)記的親和素,再次徹底洗滌后即可上機(jī)讀數(shù)。MFI值和樣品中的肌動(dòng)蛋白β(ACTB)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法)呈正相關(guān)。
贈(zèng)品
相關(guān)產(chǎn)品
編號(hào) | 適用物種:Homo sapiens (Human,人), Mus musculus (Mouse,小鼠), Rattus norvegicus (Rat,大鼠) | 應(yīng)用(僅供研究使用,不用于臨床診斷!) |
RPB340Mi01 | 肌動(dòng)蛋白β(ACTB)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
CAB340Mi01 | 抗肌動(dòng)蛋白β(ACTB)多克隆抗體 | Loading Control of WB. |
PAB340Mi01 | 肌動(dòng)蛋白β(ACTB)多克隆抗體 | WB; IHC; ICC; IP. |
MAB340Mi22 | 肌動(dòng)蛋白β(ACTB)單克隆抗體 | WB; IHC; ICC; IP. |
CAB340Mi22 | 抗肌動(dòng)蛋白β(ACTB)單克隆抗體 | Loading Control of WB |
MAB340Mi23 | 肌動(dòng)蛋白β(ACTB)單克隆抗體 | WB; IHC; ICC; IP. |
MAB340Mi21 | 肌動(dòng)蛋白β(ACTB)單克隆抗體 | WB; IHC; ICC; IP. |
MAB340Mi24 | 肌動(dòng)蛋白β(ACTB)單克隆抗體 | WB; IHC; ICC; IP. |
SEB340Mi | 肌動(dòng)蛋白β(ACTB)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
SCB340Mi | 肌動(dòng)蛋白β(ACTB)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
LMB340Mi | 肌動(dòng)蛋白β(ACTB)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
KSB340Mi01 | 肌動(dòng)蛋白β(ACTB)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) | Main materials for "Do It (ELISA Kit) Yourself". |
PGB340Mi01 | 肌動(dòng)蛋白β(ACTB)引物對(duì) | PCR |
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