肌動蛋白β(ACTB)檢測試劑盒(化學(xué)發(fā)光免疫分析法)
CLIA Kit for Beta Actin (ACTB)
Actin Beta; ACT-B; PS1TP5BP1; Actin, cytoplasmic 1; Actin, cytoplasmic 1, N-terminally processed
- 編號SCB340Mi
- 物種Homo sapiens (Human,人), Mus musculus (Mouse,小鼠), Rattus norvegicus (Rat,大鼠) 相同的名稱,不同的物種。
- 實驗方法雙抗夾心
- 反應(yīng)時長2h, 40min
- 檢測范圍0.14-100ng/mL
- 靈敏度最小可檢測劑量小于等于0.06ng/mL.
- 樣本類型tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下載 英文說明書 中文說明書
- 規(guī)格 48T96T 96T*5 96T*10 96T*100
- 價格 ¥ 3629 ¥ 5184 ¥ 23328 ¥ 44064 ¥ 362880
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特異性
本試劑盒對檢測肌動蛋白β(ACTB)具有較高的靈敏度。
經(jīng)檢測人、小鼠、大鼠之間有100%的交叉反映。
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差:取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次,分別計算不同濃度樣本的平均值及SD值。
批間差:選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定,每個樣本使用同一試劑盒重復(fù)測定8次,分別計算不同濃度樣本的平均值及SD值。
批內(nèi)差: CV<10%
批間差: CV<12%
穩(wěn)定性
經(jīng)測定,試劑盒在有效期內(nèi)按推薦溫度保存,其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響,實驗室的環(huán)境條件需盡量保持一致,尤其是實驗室內(nèi)溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。
實驗流程
1. 實驗前標準品、試劑及樣本的準備;
2. 加樣(標準品及樣本)100µL,37°C孵育1小時;
3. 吸棄,加檢測溶液A100μL,37°C孵育1小時;
4. 洗板3次;
5. 加檢測溶液B100µL,37°C孵育30分鐘;
6. 洗板5次;
7. 加底物100µL,37°C孵育10分鐘;
8. 讀數(shù)。
實驗原理
將肌動蛋白β(ACTB)抗體包被于96孔微孔板中,制成固相載體,向微孔中分別加入標準品或標本,其中的肌動蛋白β(ACTB)與連接于固相載體上的抗體結(jié)合,然后加入生物素化的肌動蛋白β(ACTB)抗體,將未結(jié)合的生物素化抗體洗凈后,加入HRP標記的親和素,再次徹底洗滌后加入底物。加入底物后會產(chǎn)生輝光型光信號。發(fā)射光強度和樣品中的肌動蛋白β(ACTB)呈正相關(guān)。用化學(xué)發(fā)光儀測定相對光單位(RLU),計算樣品濃度。
增值服務(wù)
相關(guān)產(chǎn)品
編號 | 適用物種:Homo sapiens (Human,人), Mus musculus (Mouse,小鼠), Rattus norvegicus (Rat,大鼠) | 應(yīng)用(僅供研究使用,不用于臨床診斷!) |
RPB340Mi01 | 肌動蛋白β(ACTB)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
CAB340Mi01 | 抗肌動蛋白β(ACTB)多克隆抗體 | Loading Control of WB. |
PAB340Mi01 | 肌動蛋白β(ACTB)多克隆抗體 | WB; IHC; ICC; IP. |
MAB340Mi22 | 肌動蛋白β(ACTB)單克隆抗體 | WB; IHC; ICC; IP. |
CAB340Mi22 | 抗肌動蛋白β(ACTB)單克隆抗體 | Loading Control of WB |
MAB340Mi23 | 肌動蛋白β(ACTB)單克隆抗體 | WB; IHC; ICC; IP. |
MAB340Mi21 | 肌動蛋白β(ACTB)單克隆抗體 | WB; IHC; ICC; IP. |
MAB340Mi24 | 肌動蛋白β(ACTB)單克隆抗體 | WB; IHC; ICC; IP. |
SEB340Mi | 肌動蛋白β(ACTB)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
SCB340Mi | 肌動蛋白β(ACTB)檢測試劑盒(化學(xué)發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
LMB340Mi | 肌動蛋白β(ACTB)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
KSB340Mi01 | 肌動蛋白β(ACTB)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
PGB340Mi01 | 肌動蛋白β(ACTB)引物對 | PCR |
參考文獻
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