肌動蛋白β(ACTB)單克隆抗體
Monoclonal Antibody to Beta Actin (ACTB)
Actin Beta; ACT-B; PS1TP5BP1; Actin, cytoplasmic 1; Actin, cytoplasmic 1, N-terminally processed
- 編號MAB340Mi21
- 物種Homo sapiens (Human，人), Mus musculus (Mouse，小鼠), Rattus norvegicus (Rat，大鼠), Cavia (Guinea pig ，豚鼠), Canis familiaris; Canine (Dog，犬), Sus scrofa; Porcine (Pig，豬), Bos taurus; Bovine (Cattle，牛), Ovis aries; Ovine (Sheep，綿羊), Equus caballus; Equine (Horse，馬), Chicken (Gallus，雞) 相同的名稱，不同的物種。
- 來源單抗制備
- 宿主小鼠
- 效價(jià)(克隆號)C7
- Ig亞型 IgG2b Kappa
- 純化方式蛋白A + 蛋白G親和純化
- 標(biāo)記物無標(biāo)記物
- 免疫原 RPB340Mi01-肌動蛋白β(ACTB)重組蛋白
- 緩沖液成份0.01M 磷酸鹽緩沖液（pH7.4，containing 0.05% Proclin-300，50% glycerol）
- 性狀液體
- 濃度1mg/ml
- 且適物種Oryctolagus cuniculus (Rabbit，兔), Capra hircus; Caprine (Goat，山羊)
- 應(yīng)用WB; IHC; ICC; IP.
如果抗體需用于流式細(xì)胞術(shù)，請參見流式抗體。 - 下載 英文說明書 中文說明書
- 規(guī)格 20μl100μl 200μl 1ml 10ml
- 價(jià)格 ¥ 770 ¥ 1798 ¥ 2568 ¥ 6420 ¥ 25680
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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DAB staining on IHC-P; Sample: Human Kidney Tissue; Primary Ab: 10μg/ml Mouse Anti-Multi-species ACTb Antibody Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
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DAB staining on IHC-P;
Sample: Human Stomach Tissue;
Primary Ab: 10μg/ml Mouse Anti-Multi-species ACTb Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
DAB staining on IHC-P;
Sample: Human Colon Tissue;
Primary Ab: 10μg/ml Mouse Anti-Multi-species ACTb Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
DAB staining on IHC-P;
Sample: Human Amygdalitis Tissue;
Primary Ab: 10μg/ml Mouse Anti-Multi-species ACTb Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
DAB staining on IHC-P;
Sample: Porcine Cardiac Muscle Tissue;
Primary Ab: 20μg/ml Mouse Anti-Multi-species ACTb Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
DAB staining on IHC-P;
Sample: Porcine Skeletal muscle Tissue;
Primary Ab: 20μg/ml Mouse Anti-Multi-species ACTb Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
DAB staining on IHC-P;
Sample: Porcine Heart Tissue
Primary Ab: 20μg/ml Mouse Anti-Multi-species ACTB Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
DAB staining on IHC-P;
Sample: Porcine Skeletal muscle Tissue
Primary Ab: 20μg/ml Mouse Anti-Multi-species ACTB Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
DAB staining on IHC-P;
Sample: Human Tonsil Tissue
Primary Ab: 10μg/ml Mouse Anti-Multi-species ACTB Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
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WB圖
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Western Blot; Sample: Lane1: Human Lung lysate; Lane2: 293T cell lysate; Lane3: Rat Liver lysate; Lane4: Rat Kidney lysate; Lane5: Porcine Liver lysate; Lane6: Porcine Kidney lysate; Lane7: Mouse Liver lysate
Primary Ab: 1μg/ml Mouse Anti-Multi-species ACTb Antibody
Second Ab: 0.2μg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
Western Blot; Sample: Lane1: Mouse Kidney lysate; Lane2: Rabbit Liver lysate; Lane3: Bovine Liver lysate; Lane4: Bovine Kidney lysate; Lane5: Canine Liver lysate; Lane6: Canine Kidney lysate
Primary Ab: 1μg/ml Mouse Anti-Multi-species ACTb Antibody
Second Ab: 0.2μg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
Western Blot; Sample: Lane1: Human Lung lysate; Lane2: Human Placenta lysate; Lane3: Mouse Cerebrum lysate; Lane4: Mouse Liver lysate; Lane5: Rat Cerebrum lysate; Lane6: Rat Liver lysate; Lane7: Cavia Cerebrum lysate
Primary Ab: 1μg/ml Mouse Anti-Multi-species ACTb Antibody
Second Ab: 0.2μg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
Western Blot; Sample: Lane1: Canine Cerebrum lysate; Lane2: Porcine Cerebrum lysate; Lane3: Bovine Cerebrum lysate; Lane4: Gallus Cerebrum lysate; Lane5: Equine Liver lysate; Lane6: Caprine Cerebrum lysate;
Primary Ab: 0.5μg/ml Mouse Anti-Multi-species ACTb Antibody
Second Ab: 0.2μg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
Western Blot; Sample: Lane1: DU145 cell lysate; Lane2: HL60 cell lysate; Lane3: THP1 cell lysate; Lane4: OS-RC-2 cell lysate; Lane5: HEC-1B cell lysate; Lane6: MDA-MB-435S cell lysate; Lane7: T-47D cell lysate
Primary Ab: 0.5μg/ml Mouse Anti-Multi-species ACTb Antibody
Second Ab: 0.2μg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
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通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
該抗體是針對ACTB的小鼠單克隆抗體。在免疫組織化學(xué)染色和免疫印跡實(shí)驗(yàn)中能識別ACTB。
用法
Western blotting: 0.01-2μg/mL;
Immunohistochemistry: 5-20μg/mL;
Immunocytochemistry: 5-20μg/mL;
Optimal working dilutions must be determined by end user.
儲存
經(jīng)常使用則4°C保存。-20°C保存不超過兩年。避免反復(fù)凍融。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定，具體方法如下：在37°C孵育48小時(shí)，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲，損失率低于5%。
贈品
增值服務(wù)
抗體標(biāo)記定制服務(wù)
Protein-A/G純化柱
細(xì)胞和組織染色液
抗體陽性對照品
組織/切片定制服務(wù)
磷酸化抗體定制服務(wù)
免疫印跡(WB)實(shí)驗(yàn)服務(wù)
免疫組織化學(xué)(IHC)實(shí)驗(yàn)服務(wù)
免疫細(xì)胞化學(xué)(ICC)實(shí)驗(yàn)服務(wù)
流式細(xì)胞術(shù)(FCM)實(shí)驗(yàn)服務(wù)
免疫沉淀(IP)實(shí)驗(yàn)服務(wù)
免疫熒光(IF)實(shí)驗(yàn)服務(wù)
緩沖液
DAB顯色試劑盒
SABC試劑盒
長臂生物素標(biāo)記試劑盒
實(shí)時(shí)熒光定量PCR實(shí)驗(yàn)服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Homo sapiens (Human，人), Mus musculus (Mouse，小鼠), Rattus norvegicus (Rat，大鼠), Cavia (Guinea pig ，豚鼠), Canis familiaris; Canine (Dog，犬), Sus scrofa; Porcine (Pig，豬), Bos taurus; Bovine (Cattle，牛), Ovis aries; Ovine (Sheep，綿羊), Equus caballus; Equine (Horse，馬), Chicken (Gallus，雞) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| RPB340Mi01 | 肌動蛋白β(ACTB)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| CAB340Mi01 | 抗肌動蛋白β(ACTB)多克隆抗體 | Loading Control of WB. |
| PAB340Mi01 | 肌動蛋白β(ACTB)多克隆抗體 | WB; IHC; ICC; IP. |
| MAB340Mi22 | 肌動蛋白β(ACTB)單克隆抗體 | WB; IHC; ICC; IP. |
| CAB340Mi22 | 抗肌動蛋白β(ACTB)單克隆抗體 | Loading Control of WB |
| MAB340Mi23 | 肌動蛋白β(ACTB)單克隆抗體 | WB; IHC; ICC; IP. |
| MAB340Mi21 | 肌動蛋白β(ACTB)單克隆抗體 | WB; IHC; ICC; IP. |
| MAB340Mi24 | 肌動蛋白β(ACTB)單克隆抗體 | WB; IHC; ICC; IP. |
| SEB340Mi | 肌動蛋白β(ACTB)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SCB340Mi | 肌動蛋白β(ACTB)檢測試劑盒(化學(xué)發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| LMB340Mi | 肌動蛋白β(ACTB)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| KSB340Mi01 | 肌動蛋白β(ACTB)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) | Main materials for "Do It (ELISA Kit) Yourself". |
| PGB340Mi01 | 肌動蛋白β(ACTB)引物對 | PCR |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| International Journal of Molecular Sciences | Nano-Nutrition of Chicken Embryos. The Effect of in Ovo Administration of Diamond Nanoparticles and L-Glutamine on Molecular Responses in Chicken Embryo Pectoral Muscles [NCBI:PMC3856104] |
| European Journal of BioMedical Research | Expression of chloride channels in mouse skin incisional wounds [96] |
| Cell?Metabolism | Targeted Elimination of Senescent Beta Cells Prevents Type 1 Diabetes [] |
| Biological & Pharmaceutical Bulletin | Neuroinflammation, Oxidative Stress, and Neurogenesis in a Mouse Model of Chronic Fatigue Syndrome, and the Treatment with Kampo Medicine [Pubmed: 31902915] |
| Biological research | Linc-OIP5 in the breast cancer cells regulates angiogenesis of human umbilical vein endothelial cells through YAP1/Notch/NRP1 signaling circuit at a tumor … [Pubmed: 32046779] |
| JOURNAL OF INVESTIGATIVE MEDICINE | Effect of ischemic postconditioning on cell apoptosis and expression of relevant genes in non-culprit coronary arteries [Pubmed: 32784207] |
| bioscience biotechnology research asia | Malted Barley Improved the Structure and Function of Gastrocnemius Muscle of Hypercholesterolemic Mother Rats and their Offspring [] |
| Genes | Tryptophan Depletion Modulates Tryptophanyl-tRNA Synthetase-Mediated High-Affinity Tryptophan Uptake into Human Cells [33261077] |
| Nat Commun | POLRMT mutations impair mitochondrial transcription causing neurological disease [33602924] |
| Life Sci | Indole-linked 1, 2, 3-triazole derivatives efficiently modulate COX-2 protein levels in human THP-1 monocytes by suppressing AGE-ROS-NF-kβ nexus [Pubmed:34990649] |
| J Ethnopharmacol | Isolation and characterization of anti-inflammatory and anti-proliferative compound, for B-cell Non-Hodgkin lymphoma, from Nyctanthes arbor-tristis Linn. [Pubmed:35398498] |
| Multimedia Tools and Applications | Investigation of single beam ultrasound sensitivity as a monitoring tool for local hyperthermia treatment in breast cancer [] |
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