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酸性神經(jīng)鞘磷脂酶(ASM)活性蛋白

Active Acid Sphingomyelinase (ASM)

SMPD1; NPD; SMase; aSMase; Sphingomyelin Phosphodiesterase 1,Acid Lysosomal; Simply Sphingomyelinase

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  • 酸性神經(jīng)鞘磷脂酶(ASM)活性蛋白 產(chǎn)品包裝(模擬)
  • 酸性神經(jīng)鞘磷脂酶(ASM)活性蛋白 Figure . Gene Sequencing (extract)
  • APB360Mu61.jpg Figure. SDS-PAGE
  • Certificate 通過ISO 9001、ISO 13485質量體系認證

活性實驗

Sphingomyelin phosphodiesterase 1 (SMPD1), also known as acid sphingomyelinase (ASM), belongs to the sphingomyelin phosphodiesterase family. The protein Converts sphingomyelin to ceramide. ASM also has phospholipase C activities toward 1,2-diacylglycerolphosphocholine and 1,2-diacylglycerolphosphoglycerol.

Thus, the recombinant mouse ASM activity was measured by its ability to hydrolyze 2-N-Hexadecanoylamino-4-nitrophenylphosphorylcholine (HDA-PC) to p-Nitrophenol. The reaction was performed in 50mM MES, 0.5 μM ZnCl2, pH 7.5 ( Assay Buffer), initiated by addition 50μL of various concentrations of ASM (diluted with Assay Buffer) to 50 μL of 1mM Substrate HDA-PC ( 50mM stock solution in methanol, diluted with Assay Buffer). Incubated at room temperature for 20 minutes in the dark, then read at a wavelength of 405 nm.

用法

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

儲存

避免反復凍融。2-8°C不超過一個月，-80°C不超過12個月。

穩(wěn)定性

熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產(chǎn)生。保質期內，在適當?shù)臈l件下存儲，損失率低于5%。

相關產(chǎn)品

編號 適用物種：Mus musculus (Mouse，小鼠) 應用(僅供研究使用，不用于臨床診斷！)
EPB360Mu61 酸性神經(jīng)鞘磷脂酶(ASM)真核蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APB360Mu61 酸性神經(jīng)鞘磷脂酶(ASM)活性蛋白 Cell?culture;?Activity?Assays.

參考文獻

雜志 參考文獻
Blood.? Acid sphingomyelinase is activated in sickle cell erythrocytes and contributes to inflammatory microparticle generation in SCD [Pubmed:25075126]
Journal of Clinical Lipidology Increase in acid sphingomyelinase level in human retinal endothelial cells and CD34+ circulating angiogenic cells isolated from diabetic individuals is associated with dysfunctional retinal vasculature and vascular repair process in diabetes [10.1016/j.jacl.2017.03.007]
Diabetes & metabolism Fenofibrate decreases plasma ceramide in type 2 diabetes patients: A novel marker of CVD? [pubmed:28499696]
INFLAMMATION Acid Sphingomyelinase and Acid |?-Glucosidase 1 Exert Opposite Effects on Interleukin-1|?-Induced Interleukin 6 Production in Rheumatoid Arthritis Fibroblast-Like Synoviocytes [33665756]
Indian J Ophthalmol Evaluation of sphingolipid metabolism on diabetic retinopathy [34708809]
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