脂聯(lián)素(ADPN)活性蛋白
Active Adiponectin (ADPN)
GBP28; ApM1; AdipoQ; Acrp30; ACDC; APM1; C1Q And Collagen Domain Containing; Adipocyte Complement-Related Protein Of 30 KDa; Adipose Most Abundant Gene Transcript 1
- 編號APA605Ga01
- 物種Chicken (Gallus，雞) 相同的名稱，不同的物種。
- 緩沖液成份PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
- 性狀凍干粉
- 純度> 90%
- 等電點6.4
- 應用Cell?culture;?Activity?Assays.
- 下載 英文說明書 中文說明書
- 規(guī)格 10μg50μg 200μg 1mg 5mg
- 價格 ¥ 1560 ¥ 3900 ¥ 7800 ¥ 23400 ¥ 58500
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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Figure. SDS-PAGE
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通過ISO 9001、ISO 13485質(zhì)量體系認證
活性實驗
Human Adiponectin?(ADPN) is a 53 kDa member of the PNPLA family of phospholipase A2 enzymes. It is a plasma protein secreted by adipose tissue showing pleiotropic effects with anti-diabetic, anti-atherogenic and anti-inflammatory properties. Adiponectin and AdipoR1 regulate PPARgC1a and mitochondria by Ca(2 ) and AMPK/SIRT1. Thus a functional binding ELISA assay was conducted to detect the interaction of recombinant chicken ADPN and recombinant rat PPARgC1a. Briefly, ADPN was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to PPARgC1a-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-ADPN pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37℃, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 μL stop solution to the wells and read at 450/630 nm immediately. The binding activity of recombinant chicken ADPN and recombinant rat PPARgC1a was shown in Figure 1, the EC50?for this effect is 0.12 ug/mL.
用法
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
儲存
避免反復凍融。2-8°C不超過一個月，-80°C不超過12個月。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當?shù)臈l件下存儲，損失率低于5%。
增值服務
相關產(chǎn)品
| 編號 | 適用物種：Chicken (Gallus，雞) | 應用(僅供研究使用，不用于臨床診斷！) |
| RPA605Ga01 | 脂聯(lián)素(ADPN)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APA605Ga01 | 脂聯(lián)素(ADPN)活性蛋白 | Cell?culture;?Activity?Assays. |
| PAA605Ga01 | 脂聯(lián)素(ADPN)多克隆抗體 | WB; IHC; ICC; IP. |
| MAA605Ga21 | 脂聯(lián)素(ADPN)單克隆抗體 | WB; IHC; ICC; IP. |
| SEA605Ga | 脂聯(lián)素(ADPN)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SCA605Ga | 脂聯(lián)素(ADPN)檢測試劑盒(化學發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| LMA605Ga | 脂聯(lián)素(ADPN)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
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