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羧酸酯酶1(CES1)活性蛋白

Active Carboxylesterase 1 (CES1)

ACAT; CEH; CES2; HMSE1; PCE1; SES1; TGH; Retinyl ester hydrolase; Serine esterase 1; Triacylglycerol hydrolase; Egasyn; Cocaine carboxylesterase; Liver Carboxylesterase 1

  • 羧酸酯酶1(CES1)活性蛋白 產(chǎn)品包裝(模擬)
  • 羧酸酯酶1(CES1)活性蛋白 產(chǎn)品包裝(模擬)
  • APC374Hu61.jpg Figure. SDS-PAGE
  • Certificate 通過ISO 9001、ISO 13485質量體系認證

活性實驗

carboxylesterase 1 (CES1) also known as Liver carboxylesterase 1 is a serine esterase and member of a large multigene carboxylesterase family. The protein Involved in the detoxification of xenobiotics and in the activation of ester and amide prodrugs. Hydrolyzes aromatic and aliphatic esters, but has no catalytic activity toward amides or a fatty acyl-CoA ester. Hydrolyzes the methyl ester group of cocaine to form benzoylecgonine. Thus, the recombinant human CES1 activity was measured by its ability to hydrolyze 4-Nitrophenyl acetate (4-NPA) to 4-Nitrophenol. The reaction was performed in 50 mM Tris, pH 7.5( Assay Buffer), initiated by addition 50 μL of various concentrations of CES1 (dilute by Assay Buffer) to 50 μL of 2 mM Substrate 4-NPA(100 mM stock in Acetone, dilute by deionized water). Incubated at 37℃ for 10min, then read at a wavelength of 400 nm.

用法

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

儲存

避免反復凍融。2-8°C不超過一個月,-80°C不超過12個月。

穩(wěn)定性

熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定,具體方法如下:在37°C孵育48小時,沒有顯著的降解或者沉淀產(chǎn)生。保質期內,在適當?shù)臈l件下存儲,損失率低于5%。

相關產(chǎn)品

編號 適用物種:Homo sapiens (Human,人) 應用(僅供研究使用,不用于臨床診斷!)
EPC374Hu61 羧酸酯酶1(CES1)真核蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APC374Hu01 羧酸酯酶1(CES1)活性蛋白 Cell?culture;?Activity?Assays.
RPC374Hu01 羧酸酯酶1(CES1)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APC374Hu61 羧酸酯酶1(CES1)活性蛋白 Cell?culture;?Activity?Assays.
PAC374Hu01 羧酸酯酶1(CES1)多克隆抗體 WB; IHC; ICC; IP.
SEC374Hu 羧酸酯酶1(CES1)檢測試劑盒(酶聯(lián)免疫吸附試驗法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMC374Hu 羧酸酯酶1(CES1)等多因子檢測試劑盒(流式熒光發(fā)光法) FLIA Kit for Antigen Detection.

參考文獻

雜志 參考文獻
International Journal of Nanomedicine Gold Nanoparticles Perturb Drug-Metabolizing Enzymes and Antioxidants in the Livers of Male Rats: Potential Impact on Drug Interactions [Pubmed: 32764932]
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