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羧酸酯酶2(CES2)活性蛋白

Active Carboxylesterase 2 (CES2)

CE-2; CES2A1; PCE-2; iCE; Intestine,Liver; Methylumbelliferyl-acetate deacetylase 2; Cocaine esterase

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  • 羧酸酯酶2(CES2)活性蛋白 產(chǎn)品包裝(模擬)
  • APF282Hu61.jpg SDS-PAGE圖
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活性實(shí)驗(yàn)

Carboxylesterase 2 (CES2) is a member of serine esterase family which can hydrolyze ester and amide bonds. The protein Involved in the detoxification of xenobiotics and in the activation of ester and amide prodrugs. Hydrolyzes aromatic and aliphatic esters, but has no catalytic activity toward amides or a fatty acyl-CoA ester. Hydrolyzes the methyl ester group of cocaine to form benzoylecgonine. Thus, the recombinant human CES2 activity was measured by its ability to hydrolyze 4-Nitrophenyl acetate (4-NPA) to 4-Nitrophenol. The reaction was performed in 50 mM Tris, 150 mM NaCl, pH 7.5 (Assay Buffer), ainitiated by addition 50 μL of various concentrations of CES2 (diluted by Assay Buffer) to 50 μL of 2 mM Substrate 4-NPA (100 mM stock in Acetone, diluted by Assay Buffer). Incubated at 37℃ for 10 min, then read at a wavelength of 400 nm.

用法

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

儲(chǔ)存

避免反復(fù)凍融。2-8°C不超過一個(gè)月，-80°C不超過12個(gè)月。

穩(wěn)定性

熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定，具體方法如下：在37°C孵育48小時(shí)，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲(chǔ)，損失率低于5%。

相關(guān)產(chǎn)品

編號(hào) 適用物種：Homo sapiens (Human，人) 應(yīng)用(僅供研究使用，不用于臨床診斷！)
RPF282Hu01 羧酸酯酶2(CES2)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APF282Hu61 羧酸酯酶2(CES2)活性蛋白 Cell?culture;?Activity?Assays.
EPF282Hu61 羧酸酯酶2(CES2)真核蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
WEF282Hu 羧酸酯酶2(CES2)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,寬范圍) Enzyme-linked immunosorbent assay for Antigen Detection.
SEF282Hu 羧酸酯酶2(CES2)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMF282Hu 羧酸酯酶2(CES2)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) FLIA Kit for Antigen Detection.

參考文獻(xiàn)

雜志 參考文獻(xiàn)
Metallomics Comprehensive and comparative exploration of the Atp7b?/? mouse plasma proteome [Pubmed: 31815268]
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