上皮性鈣黏附蛋白活性蛋白
Active E-cadherin
CDHE; CD324; CDH1; Arc1; ECAD; CDH-E; E-CAD; LCAM; UVO; Cadherin 1 Type 1; Uvomorulin; E-Cadherin; Calcium-Dependent Adhesion Protein, Epithelial; Liver Cell Adhesion Molecule
- 編號(hào)APA017Hu61
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 緩沖液成份PBS, pH7.4, containing 5% Trehalose.
- 性狀凍干粉
- 純度> 95%
- 等電點(diǎn)4.0
- 應(yīng)用Cell?culture;?Activity?Assays.
- 下載 英文說(shuō)明書(shū) 中文說(shuō)明書(shū)
- 規(guī)格 10μg50μg 200μg 1mg 5mg
- 價(jià)格 ¥ 2227 ¥ 5568 ¥ 11136 ¥ 33408 ¥ 83520
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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Figure. SDS-PAGE
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通過(guò)ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
活性實(shí)驗(yàn)
E-Cadherin, also known as Uvomorulin in the mouse and rat, is a 120?kDa member of the Cadherin family of cell surface glycoproteins that mediate cell adhesion. Human E-Cadherin shares 81%?amino acid sequence identity with the rat and mouse proteins. It is a single-pass transmembrane protein that mediates calcium-dependent epithelial cell adhesion. As E-cadherin has the function of cell adhesion, we measure the activity of recombinant human E-cadherin by the ability of the immobilized protein to support the adhesion of MCF7 human breast cancer cells. When 5 x 104?cells/well are added to different concentrations of recombinat human E-cadherin coated plates, cells will adhere after 2?hour incubation at 37 ℃. The adhesion of MCF7 after 2?hour incubation at 37 ℃ observed by inverted microscope was shown in Figure 1. Cell adherent was in a dose dependent manner, the result was shown in Figure 2, the EC50 was 0.19 ug/ml.
用法
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
儲(chǔ)存
避免反復(fù)凍融。2-8°C不超過(guò)一個(gè)月，-80°C不超過(guò)12個(gè)月。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定，具體方法如下：在37°C孵育48小時(shí)，沒(méi)有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲(chǔ)，損失率低于5%。
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| 編號(hào) | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| APA017Hu62 | 上皮性鈣黏附蛋白活性蛋白 | Cell?culture;?Activity?Assays. |
| RPA017Hu01 | 上皮性鈣黏附蛋白重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APA017Hu61 | 上皮性鈣黏附蛋白活性蛋白 | Cell?culture;?Activity?Assays. |
| EPA017Hu62 | 上皮性鈣黏附蛋白真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| EPA017Hu61 | 上皮性鈣黏附蛋白真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA017Hu01 | 上皮性鈣黏附蛋白多克隆抗體 | WB; IHC; ICC; IP. |
| PAA017Hu02 | 上皮性鈣黏附蛋白多克隆抗體 | WB; IHC; ICC; IP. |
| LAA017Hu71 | 上皮性鈣黏附蛋白多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| LAA017Hu81 | 上皮性鈣黏附蛋白多克隆抗體(異硫氰酸熒光素標(biāo)記) | WB; IHC; ICC; IF. |
| MAA017Hu23 | 上皮性鈣黏附蛋白單克隆抗體 | WB; IHC; ICC; IP. |
| MAA017Hu22 | 上皮性鈣黏附蛋白單克隆抗體 | WB; IHC; ICC; IP. |
| MAA017Hu21 | 上皮性鈣黏附蛋白單克隆抗體 | WB; IHC; ICC; IP. |
| MAA017Hu24 | 上皮性鈣黏附蛋白單克隆抗體 | WB; IHC; ICC; IP. |
| SEA017Hu | 上皮性鈣黏附蛋白檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SCA017Hu | 上皮性鈣黏附蛋白檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| LMA017Hu | 上皮性鈣黏附蛋白等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| J Periodontal Res. | Novel method for proliferation of oral keratinocyte stem cells [Pubmed: 24329011] |
| The Journal of?Gene?Medicine | Modulation of the Dual‐Faced Effects of miR‐141 with Chitosan/miR‐141 Nanoplexes in Breast Cancer Cells [Pubmed: 31389101] |
| Cell Adhesion & Migration | BST2 regulates interferon gamma-dependent decrease in invasion of HTR-8/SVneo cells via STAT1 and AKT signaling pathways and expression of E-cadherin [Pubmed: 31957537] |
| DOKLADY BIOCHEMISTRY AND BIOPHYSICS | Personalized Approach to Determination of Histidine-Rich Glycoprotein and E-Cadherin in Supernatants of Immunocompetent Blood Cells and Breast Biopsy … [Pubmed: 32342301] |
| Non-POU Domain-Containing Octamer-Binding (NONO) Protein Stability Regulated by PIN1 is Crucial for Breast Cancer Tumorigenicity Via the MAPK/β?… [] |
