凋亡相關(guān)因子(FAS)活性蛋白
Active Factor Related Apoptosis (FAS)
CD95; ALPS1A; ALPS1-A; APO1; APT1; FAS1; FASTM; TNFRSF6; Fas Receptor; TNF Receptor Superfamily Member 6; Tumor Necrosis Factor Receptor Superfamily Member 6
- 編號APA030Hu01
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 緩沖液成份20mM Tris, 150mM NaCl緩沖液（pH8.0, 含有1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose和Proclin300）
- 性狀凍干粉
- 純度> 95%
- 等電點6.7
- 應(yīng)用Cell?culture;?Activity?Assays.
- 下載 英文說明書 中文說明書
- 規(guī)格 10μg50μg 200μg 1mg 5mg
- 價格 ¥ 1642 ¥ 4104 ¥ 8208 ¥ 24624 ¥ 61560
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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Figure. SDS-PAGE
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通過ISO 9001、ISO 13485質(zhì)量體系認證
活性實驗
FAS (Tumor necrosis factor receptor superfamily member 6) belongs to the tumor necrosis factor receptor superfamily. FAS contains a death domain, which has been shown to play a central role in the physiological regulation of programmed cell death. A binding ELISA assay was conducted to detect the association of FAS with FASL. Briefly, FASL were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul FASL were then transferred to FAS-coated microtiter wells (1ug/ml, 100ul/well) and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-FASL pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of FAS and FASL was shown in Figure 1, and this effect was in a dose dependent manner, the EC50 was approximately 0.012 ug/mL.
用法
Reconstitute in 20mM Tris, 150mM NaCl (PH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
儲存
避免反復(fù)凍融。2-8°C不超過一個月，-80°C不超過12個月。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲，損失率低于5%。
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| RPA030Hu02 | 凋亡相關(guān)因子(FAS)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA030Hu03 | 凋亡相關(guān)因子(FAS)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA030Hu01 | 凋亡相關(guān)因子(FAS)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APA030Hu03 | 凋亡相關(guān)因子(FAS)活性蛋白 | Cell?culture;?Activity?Assays. |
| EPA030Hu61 | 凋亡相關(guān)因子(FAS)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APA030Hu61 | 凋亡相關(guān)因子(FAS)活性蛋白 | Cell?culture;?Activity?Assays. |
| APA030Hu01 | 凋亡相關(guān)因子(FAS)活性蛋白 | Cell?culture;?Activity?Assays. |
| PAA030Hu02 | 凋亡相關(guān)因子(FAS)多克隆抗體 | WB; IHC |
| PAA030Hu03 | 凋亡相關(guān)因子(FAS)多克隆抗體 | WB; IHC; ICC; IP. |
| PAA030Hu01 | 凋亡相關(guān)因子(FAS)多克隆抗體 | WB; IHC; ICC; IP. |
| FAA030Hu01 | 抗凋亡相關(guān)因子(FAS)多克隆抗體 | Flow cytometry. |
| LAA030Hu71 | 凋亡相關(guān)因子(FAS)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| LAA030Hu81 | 凋亡相關(guān)因子(FAS)多克隆抗體(異硫氰酸熒光素標(biāo)記) | WB; IHC; ICC; IF. |
| FAA030Hu41 | 抗凋亡相關(guān)因子(FAS)多克隆抗體(藻紅素標(biāo)記) | Flow cytometry. |
| FAA030Hu51 | 抗凋亡相關(guān)因子(FAS)多克隆抗體(別藻藍蛋白標(biāo)記) | Flow cytometry. |
| FAA030Hu81 | 抗凋亡相關(guān)因子(FAS)多克隆抗體(異硫氰酸熒光素標(biāo)記) | Flow cytometry. |
| MAA030Hu22 | 凋亡相關(guān)因子(FAS)單克隆抗體 | WB; IHC; ICC; IP. |
| SEA030Hu | 凋亡相關(guān)因子(FAS)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SCA030Hu | 凋亡相關(guān)因子(FAS)檢測試劑盒(化學(xué)發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| LMA030Hu | 凋亡相關(guān)因子(FAS)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| KSA030Hu01 | 凋亡相關(guān)因子(FAS)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻
| 雜志 | 參考文獻 |
| European Journal of Pharmacology | Montelukast abrogates rhabdomyolysis-induced acute renal failure via rectifying detrimental changes in antioxidant profile and systemic cytokines and apoptotic factors production [ScienceDirect: S0014299912002580] |
| J Sci Food Agric. | Beneficial effects of Allium sativum L. stem extract on lipid metabolism and antioxidant status in obese mice fed a high fat diet [Wiley: Source] |
| Tumor Biology | The diplotype Fas? 1377A/? 670G as a genetic marker to predict a lower risk of breast cancer in Chinese women [Springer:Source] |
| Naunyn Schmiedebergs Arch Pharmacol | The multi-kinase inhibitor pazopanib targets hepatic stellate cell activation and apoptosis alleviating progression of liver fibrosis [PubMed: 26269412] |
| Pharmacological Reports | The heat shock protein 90 inhibitor, 18-AAG, attenuates thioacetamide induced liver fibrosis in mice [Science: Article] |
| PLoS One | Aronia melanocarpa?Extract Ameliorates Hepatic Lipid Metabolism through PPARγ2 Downregulation [10.1371] |
| Molecular Diagnosis & Therapy | The Utility of Biomarkers in Osteoporosis Management [pubmed:28271451] |
| PLoS One | Aronia melanocarpa Extract Ameliorates Hepatic Lipid Metabolism through PPARγ2 Downregulation [pubmed:28081181] |
| Journal of Microbiology, Immunology and Infection | Identification of a panel of serum protein markers in early stage of sepsis and its validation in a cohort of patients [pubmed:28655573] |
| Journal of Functional Foods | Polysaccharides from Cyclocarya paliurus: Chemical composition and lipid-lowering effect on rats challenged with high-fat diet [10.1016/j.jff.2017.07.020] |
| Toxics | Zinc Oxide Nanoparticles Induced Oxidative DNA Damage, Inflammation and Apoptosis in Rat's Brain after Oral Exposure [Pubmed:29861430] |
| Indian?Journal?of?Medical?and?Paediatric?Oncology | The correlation between the level of doxorubicin-induced cardiac damage and serum soluble Fas in an experimental rat model [Doi: 10.4103/ijmpo.ijmpo_82_17] |
| Scientific Reports | EGF-mediated reduced miR-92a-1-5p controls HTR-8/SVneo cell invasion through activation of MAPK8 and FAS which in turn increase MMP-2/-9 expression [Pubmed: 32703964] |
