IgG-Fc片段低親和力受體Ⅲa(FcgR3A)活性蛋白
Active Fc Fragment Of IgG Low Affinity IIIa Receptor (FcgR3A)
CD16a; CD16-A; FCGR3-A; FCG3; FcgRIII; IGFR3; NA; Neutrophil Antigen; Low affinity immunoglobulin gamma Fc region receptor III-A
- 編號APB278Mu02
- 物種Mus musculus (Mouse，小鼠) 相同的名稱，不同的物種。
- 緩沖液成份PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
- 性狀凍干粉
- 純度> 90%
- 等電點7.0
- 應(yīng)用Cell?culture;?Activity?Assays.
- 下載 英文說明書 中文說明書
- 規(guī)格 10μg50μg 200μg 1mg 5mg
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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Figure. SDS-PAGE
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通過ISO 9001、ISO 13485質(zhì)量體系認證
活性實驗
Fc receptor with low affinity for IgG (Fc gamma RIII, CD16) is encoded by two nearly identical genes, Fc gamma RIII-A and Fc gamma RIII-B, resulting in tissue-specific expression of alternative membrane-anchored isoforms. FcgR3A is expressed in almost all white blood cells and is primarily responsible for the ADCC action of NK cells and the clearance of antibody-antigen immune complexes by macrophages. FcgR3B is mainly expressed in neutrophils and can stimulate the release of superoxide in neutrophils. A functional binding ELISA assay was conducted to detect the interaction of recombinant mouse FcgR3A and mouse IgG. Briefly, biotin-linked FcgR3A were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to IgG-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST 3 times and incubation with Streptavidin-HRP for 30min, then wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 μl stop solution to the wells and read at 450 nm immediately. The binding activity of recombinant mouse FcgR3A and mouse IgG was shown in Figure 1, the EC50?for this effect is 0.26 ug/mL.
用法
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
儲存
避免反復(fù)凍融。2-8°C不超過一個月，-80°C不超過12個月。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲，損失率低于5%。
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Mus musculus (Mouse，小鼠) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| RPB278Mu02 | IgG-Fc片段低親和力受體Ⅲa(FcgR3A)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| EPB278Mu61 | IgG-Fc片段低親和力受體Ⅲa(FcgR3A)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APB278Mu02 | IgG-Fc片段低親和力受體Ⅲa(FcgR3A)活性蛋白 | Cell?culture;?Activity?Assays. |
| RPB278Mu01 | IgG-Fc片段低親和力受體Ⅲa(FcgR3A)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAB278Mu01 | IgG-Fc片段低親和力受體Ⅲa(FcgR3A)多克隆抗體 | WB; IHC; ICC; IP. |
| LAB278Mu81 | IgG-Fc片段低親和力受體Ⅲa(FcgR3A)多克隆抗體(異硫氰酸熒光素標記) | WB; IHC; ICC; IF. |
| LAB278Mu71 | IgG-Fc片段低親和力受體Ⅲa(FcgR3A)多克隆抗體(生物素標記) | WB; IHC; ICC. |
參考文獻
| 雜志 | 參考文獻 |
| Scientific Reports | Specific Plasma Autoantibody Reactivity in Myelodysplastic Syndromes [Nature: Source] |
| PLoS One | Elevated Membrane and Soluble CD64: A Novel Marker Reflecting Altered FcγR Function and Disease in Early Rheumatoid Arthritis That Can Be Regulated by Anti-Rheumatic Treatment [PubMed: 26406605] |
