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半乳糖凝集素8(GAL8)活性蛋白

Active Galectin 8 (GAL8)

LGALS8; PCTA1; Po66-CBP; Prostate Carcinoma Tumor Antigen 1; Lectin,Galactoside-Binding Soluble 8; Po66 carbohydrate-binding protein; Prostate carcinoma tumor antigen 1

  • 半乳糖凝集素8(GAL8)活性蛋白 產(chǎn)品包裝(模擬)
  • 半乳糖凝集素8(GAL8)活性蛋白 產(chǎn)品包裝(模擬)
  • APA308Bo01.png Figure. SDS-PAGE
  • Certificate 通過ISO 9001、ISO 13485質(zhì)量體系認證

活性實驗

Galectin 8 (GAL8), also known as prostate carcinoma tumor antigen 1 (PCTA1) in human, is a tandem repeat-type galectin. is a member of the lectin family, of which 14 mammalian galectins have been identified. It is also a member of the beta-galactoside-binding protein family that plays an important role in cell-cell adhesion, cell-matrix interactions, macrophage activation, angiogenesis, metastasis, apoptosis. In this case, we chose rabbit erythrocyte (RaE) to assay its ability of agglutination. A general procedure for hemagglutination assay (or haemagglutination assay; HA) is as follows, two-fold dilute the recombinant Bovine GAL8 with 0.9% sodium chloride injection, add 50μL a serial dilution of GAL8 to each well of a U or V-bottom shaped 96-well microtiter plate. The final well serves as a negative control with no GAL8, replace with 50μL 0.9% sodium chloride injection. Then add 50μL 1% rabbit erythrocyte to each well and mixed gently. The plate is incubated for 3 hours at room temperature. The results are shown in Figure 1. It was obvious that the minimal effective concentration of GAL8 is 0.781 μg/mL.

用法

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

儲存

避免反復(fù)凍融。2-8°C不超過一個月，-80°C不超過12個月。

穩(wěn)定性

熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲，損失率低于5%。

相關(guān)產(chǎn)品

編號 適用物種：Bos taurus; Bovine (Cattle，牛) 應(yīng)用(僅供研究使用，不用于臨床診斷！)
RPA308Bo01 半乳糖凝集素8(GAL8)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APA308Bo01 半乳糖凝集素8(GAL8)活性蛋白 Cell?culture;?Activity?Assays.
PAA308Bo01 半乳糖凝集素8(GAL8)多克隆抗體 WB; IHC; ICC; IP.
MAA308Bo21 半乳糖凝集素8(GAL8)單克隆抗體 WB; IHC; ICC; IP.
SEA308Bo 半乳糖凝集素8(GAL8)檢測試劑盒(酶聯(lián)免疫吸附試驗法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA308Bo 半乳糖凝集素8(GAL8)等多因子檢測試劑盒(流式熒光發(fā)光法) FLIA Kit for Antigen Detection.

參考文獻

雜志 參考文獻
Cell Metabolism Podoplanin-Expressing Macrophages Promote Lymphangiogenesis and Lymphoinvasion in Breast Cancer [Pubmed: 31447322]
CALCIFIED TISSUE INTERNATIONAL The Dysregulated Galectin Network Activates NF-κB to Induce Disease Markers and Matrix Degeneration in 3D Pellet Cultures of Osteoarthritic Chondrocytes [Pubmed: 33185768]
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