谷胱甘肽S轉(zhuǎn)移酶π1(GSTp)活性蛋白
Active Glutathione S Transferase Pi (GSTp)
GSTP1; GST-P1; DFN7; FAEES3; GST3
- 編號APB090Ha01
- 物種Hamster (Chinese hamster，倉鼠) 相同的名稱，不同的物種。
- 緩沖液成份PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
- 性狀凍干粉
- 純度> 90%
- 等電點7.9
- 應(yīng)用Cell?culture;?Activity?Assays.
- 下載 英文說明書 中文說明書
- 規(guī)格 10μg50μg 200μg 1mg 5mg
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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Figure. SDS-PAGE
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通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
活性實驗
GSTp (Glutathione S-transferase P) is an enzyme that plays an important role in detoxification by catalyzing the conjugation of many hydrophobic and electrophilic compounds with reduced glutathione. GSTP1 is identified as a CDK5 (Cyclin dependent kinase-5) regulatory protein, and is thought to regulates negatively CDK5 activity via p25/p35 translocation. GSTP1 catalyze the endogenous glutathione conjugation 1-Chloro-2,4-dinitrobenzene (CDNB), which can increase in the absorbance at 340 nm. The reaction was performed in adding 10 μl 200 mM glutathione (reduced) and 10 μl 100 mM CDNB in 980 μl 100 mM NaH2PO4 (pH7.0), rapidly mixed. Then add 50 μl mixed substrates to 50 μl different concentrations of recombinant chinese hamster GSTP1, mix gentliy. Incubated at 37℃ for 5min, then read at a wavelength of 340 nm. The specific activity of recombinant chinese hamster GSTp1 is >6500 pmol/min/μg.
用法
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
儲存
避免反復(fù)凍融。2-8°C不超過一個月，-80°C不超過12個月。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲，損失率低于5%。
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Hamster (Chinese hamster，倉鼠) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| RPB090Ha01 | 谷胱甘肽S轉(zhuǎn)移酶π1(GSTp)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APB090Ha01 | 谷胱甘肽S轉(zhuǎn)移酶π1(GSTp)活性蛋白 | Cell?culture;?Activity?Assays. |
| PAB090Ha01 | 谷胱甘肽S轉(zhuǎn)移酶π1(GSTp)多克隆抗體 | WB; IHC; ICC; IP. |
| MAB090Ha21 | 谷胱甘肽S轉(zhuǎn)移酶π1(GSTp)單克隆抗體 | WB. |
| MAB090Ha22 | 谷胱甘肽S轉(zhuǎn)移酶π1(GSTp)單克隆抗體 | WB; IHC; ICC; IP. |
| MAB090Ha23 | 谷胱甘肽S轉(zhuǎn)移酶π1(GSTp)單克隆抗體 | WB; IHC; ICC; IP. |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| International Journal of Medical Sciences | Glutathione S-Transferase P1 Correlated with Oxidative Stress in Hepatocellular Carcinoma [PubMed: PMC3619117] |
| Mol Neurobiol | Human Glutathione S-Transferase Enzyme Gene Polymorphisms and Their Association With Neurocysticercosis [Pubmed:27021019] |
| Journal of Biomaterials and Tissue Engineering | 3D Printing of Differentiated Bone Marrow Mesenchymal Cells as a New Method for Liver Tissue Engineering [art00007] |
| Neuroscience?Bulletin | Comprehensive proteomic profiling of patients' tears identifies potential biomarkers for the traumatic vegetative state [Pubmed:30019218] |
| Arabian Journal of Chemistry | Attenuating effect of Indole-3-Carbinol on gold nanoparticle induced hepatotoxicity in rats [] |
| International Journal of Nature and Life Sciences | The Role of Glutathione S-Transferases in Pleomorphic Adenomas of the Salivary Glands [] |
