谷胱甘肽S轉(zhuǎn)移酶π1(GSTp)活性蛋白
Active Glutathione S Transferase Pi (GSTp)
GSTP1; GST-P1; DFN7; FAEES3; GST3
- 編號(hào)APB090Hu01
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 緩沖液成份20mM Tris, 150mM NaCl緩沖液（pH8.0, 含有1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose和Proclin300）
- 性狀凍干粉
- 純度> 95%
- 等電點(diǎn)6.2
- 應(yīng)用Cell?culture;?Activity?Assays.
- 下載 英文說明書 中文說明書
- 規(guī)格 10μg50μg 200μg 1mg 5mg
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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Figure . Gene Sequencing (extract)
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Figure. SDS-PAGE
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WB圖
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通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
活性實(shí)驗(yàn)
GSTp (Glutathione S-transferase P) is an enzyme that plays an important role in detoxification by catalyzing the conjugation of many hydrophobic and electrophilic compounds with reduced glutathione. GSTP1 is identified as a CDK5 (Cyclin dependent kinase-5) regulatory protein, and is thought to regulates negatively CDK5 activity via p25/p35 translocation. Thus a binding ELISA assay was conducted to detect the interaction of recombinant human GSTp and recombinant human CDK5. Briefly, GSTp were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100ul were then transferred to CDK5-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1 h with anti-GSTp pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 μL stop solution to the wells and read at 450 nm immediately. The binding activity of of GSTp and CDK5 was shown in Figure 1, and this effect was in a dose dependent manner.
用法
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
儲(chǔ)存
避免反復(fù)凍融。2-8°C不超過一個(gè)月，-80°C不超過12個(gè)月。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定，具體方法如下：在37°C孵育48小時(shí)，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲(chǔ)，損失率低于5%。
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| 編號(hào) | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| RPB090Hu02 | 谷胱甘肽S轉(zhuǎn)移酶π1(GSTp)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPB090Hu01 | 谷胱甘肽S轉(zhuǎn)移酶π1(GSTp)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APB090Hu01 | 谷胱甘肽S轉(zhuǎn)移酶π1(GSTp)活性蛋白 | Cell?culture;?Activity?Assays. |
| APB090Hu02 | 谷胱甘肽S轉(zhuǎn)移酶π1(GSTp)活性蛋白 | Cell?culture;?Activity?Assays. |
| PAB090Hu02 | 谷胱甘肽S轉(zhuǎn)移酶π1(GSTp)多克隆抗體 | WB; IHC; ICC; IP; FCM |
| PAB090Hu01 | 谷胱甘肽S轉(zhuǎn)移酶π1(GSTp)多克隆抗體 | WB; IHC; ICC/IF; FCM |
| LAB090Hu81 | 谷胱甘肽S轉(zhuǎn)移酶π1(GSTp)多克隆抗體(異硫氰酸熒光素標(biāo)記) | WB; IHC; ICC; IF. |
| LAB090Hu71 | 谷胱甘肽S轉(zhuǎn)移酶π1(GSTp)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| MAB090Hu21 | 谷胱甘肽S轉(zhuǎn)移酶π1(GSTp)單克隆抗體 | WB; IHC; ICC; IP. |
| MAB090Hu22 | 谷胱甘肽S轉(zhuǎn)移酶π1(GSTp)單克隆抗體 | WB; IHC; ICC; IP. |
| MAB090Hu23 | 谷胱甘肽S轉(zhuǎn)移酶π1(GSTp)單克隆抗體 | WB; IHC; ICC; IP. |
| MAB090Hu24 | 谷胱甘肽S轉(zhuǎn)移酶π1(GSTp)單克隆抗體 | WB; IHC; ICC; IP. |
| MAB090Hu25 | 谷胱甘肽S轉(zhuǎn)移酶π1(GSTp)單克隆抗體 | WB; IHC; ICC; IP. |
| LAB090Hu72 | 谷胱甘肽S轉(zhuǎn)移酶π1(GSTp)單克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| LAB090Hu82 | 谷胱甘肽S轉(zhuǎn)移酶π1(GSTp)單克隆抗體(異硫氰酸熒光素標(biāo)記) | WB; IHC; ICC; IF. |
| WEB090Hu | 谷胱甘肽S轉(zhuǎn)移酶π1(GSTp)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,寬范圍) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SEB090Hu | 谷胱甘肽S轉(zhuǎn)移酶π1(GSTp)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMB090Hu | 谷胱甘肽S轉(zhuǎn)移酶π1(GSTp)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| KSB090Hu01 | 谷胱甘肽S轉(zhuǎn)移酶π1(GSTp)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) | 自己動(dòng)手制作ELISA試劑盒的主要材料 |
| PGB090Hu01 | 谷胱甘肽S轉(zhuǎn)移酶π1(GSTp)引物對(duì) | PCR |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| International Journal of Medical Sciences | Glutathione S-Transferase P1 Correlated with Oxidative Stress in Hepatocellular Carcinoma [PubMed: PMC3619117] |
| Mol Neurobiol | Human Glutathione S-Transferase Enzyme Gene Polymorphisms and Their Association With Neurocysticercosis [Pubmed:27021019] |
| Journal of Biomaterials and Tissue Engineering | 3D Printing of Differentiated Bone Marrow Mesenchymal Cells as a New Method for Liver Tissue Engineering [art00007] |
| Neuroscience?Bulletin | Comprehensive proteomic profiling of patients' tears identifies potential biomarkers for the traumatic vegetative state [Pubmed:30019218] |
| Arabian Journal of Chemistry | Attenuating effect of Indole-3-Carbinol on gold nanoparticle induced hepatotoxicity in rats [] |
| International Journal of Nature and Life Sciences | The Role of Glutathione S-Transferases in Pleomorphic Adenomas of the Salivary Glands [] |
