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組氨酸豐富糖蛋白(HRG)活性蛋白

Active Histidine Rich Glycoprotein (HRG)

HPRG; HRGP; Histidine-Proline Rich Glycoprotein; Thrombophilia Due To Elevated HRG

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  • 組氨酸豐富糖蛋白(HRG)活性蛋白 產(chǎn)品包裝(模擬)
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  • APC534Hu61.jpg Figure. SDS-PAGE
  • 組氨酸豐富糖蛋白(HRG)活性蛋白 WB圖
  • Certificate 通過(guò)ISO 9001、ISO 13485質(zhì)量體系認(rèn)證

活性實(shí)驗(yàn)

Human histidine-rich glycoprotein (HRG) is a multidomain, monomeric, secreted, 67-75 kDa member of the cystatin superfamily of molecules. Its name derives from the fact that 26% of its amino acids (aa) are histidine and proline. In human, it is synthesized as a 525?aa precursor that contains an 18 aa signal sequence and a 507 aa mature region. Five distinct domains are recognized in the mature molecule. As HRG has the function of cell adhesion, we measure the activity of recombinant human HRG by the ability of the immobilized protein to support the adhesion of con-A activated MOLT4 human acute lymphoblastic leukemia cells. When 1 x 105?cells/well with 7.5 ug/ml conA are added to recombinat human HRG coated plates (0.1 μg/mL with 100 μL/well), >70% cells will adhere after 2?hour incubation at 37 ℃. The adhesion of MOLT-4 after 2?hour incubation at 37 ℃ observed by inverted microscope was shown in Figure 1.

用法

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

儲(chǔ)存

避免反復(fù)凍融。2-8°C不超過(guò)一個(gè)月，-80°C不超過(guò)12個(gè)月。

穩(wěn)定性

熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定，具體方法如下：在37°C孵育48小時(shí)，沒(méi)有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲(chǔ)，損失率低于5%。

相關(guān)產(chǎn)品

編號(hào) 適用物種：Homo sapiens (Human，人) 應(yīng)用(僅供研究使用，不用于臨床診斷！)
RPC534Hu01 組氨酸豐富糖蛋白(HRG)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APC534Hu61 組氨酸豐富糖蛋白(HRG)活性蛋白 Cell?culture;?Activity?Assays.
EPC534Hu61 組氨酸豐富糖蛋白(HRG)真核蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAC534Hu01 組氨酸豐富糖蛋白(HRG)多克隆抗體 WB; IHC; ICC; IP.
LAC534Hu71 組氨酸豐富糖蛋白(HRG)多克隆抗體(生物素標(biāo)記) WB; IHC; ICC.
MAC534Hu24 組氨酸豐富糖蛋白(HRG)單克隆抗體 WB; IHC; ICC; IP.
MAC534Hu22 組氨酸豐富糖蛋白(HRG)單克隆抗體 WB; IHC; ICC; IP.
MAC534Hu21 組氨酸豐富糖蛋白(HRG)單克隆抗體 WB; IHC; ICC; IP.
MAC534Hu23 組氨酸豐富糖蛋白(HRG)單克隆抗體 WB; IHC; ICC; IP.
MAC534Hu26 組氨酸豐富糖蛋白(HRG)單克隆抗體 WB; IHC; ICC; IP.
SEC534Hu 組氨酸豐富糖蛋白(HRG)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMC534Hu 組氨酸豐富糖蛋白(HRG)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) FLIA Kit for Antigen Detection.

參考文獻(xiàn)

雜志 參考文獻(xiàn)
J. Proteome Res. Identification and Confirmation of Differentially Expressed Fucosylated Glycoproteins in the Serum of Ovarian Cancer Patients Using a Lectin Array and LC–MS/MS [ACS: pr300330z]
Medical Oncology Evaluation of histidine-rich glycoprotein tissue RNA and serum protein as novel markers for breast cancer [Springer: Source]
Int J Cancer The nuclear bile acid receptor FXR controls the liver derived tumor suppressor histidine‐rich glycoprotein [PubMed: 25363753]
Nucleic Acid Therapeutics Pharmacokinetic and Pharmacodynamic Investigations of ION-353382, a Model Antisense Oligonucleotide: Using Alpha-2-Macroglobulin and Murinoglobulin Double-Knockout Mice [Pubmed:27031383]
United States Patent Application Compositions and Methods for Diagnosing Lung Cancer [y2016:0077095.html]
Hypertension?Research Combination of serum histidine-rich glycoprotein and uterine artery Doppler to predict preeclampsia [Pubmed:29416108]
journal of histochemistry & cytochemistry Ultrastructural Localization of Histidine-rich Glycoprotein in Skeletal Muscle Fibers: Colocalization With AMP Deaminase [Pubmed: 31880188]
DOKLADY BIOCHEMISTRY AND BIOPHYSICS Personalized Approach to Determination of Histidine-Rich Glycoprotein and E-Cadherin in Supernatants of Immunocompetent Blood Cells and Breast Biopsy … [Pubmed: 32342301]
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