低氧誘導因子1α(HIF1a)活性蛋白
Active Hypoxia Inducible Factor 1 Alpha (HIF1a)
HIF1-A; MOP1; PASD8; Basic Helix-Loop-Helix Transcription Factor; ARNT-interacting protein; Basic-helix-loop-helix-PAS protein MOP1; Class E basic helix-loop-helix protein 78
- 編號APA798Hu02
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 緩沖液成份20mM Tris, 150mM NaCl緩沖液（pH8.0, 含有1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose和Proclin300）
- 性狀凍干粉
- 純度> 95%
- 等電點5.0
- 應用Cell?culture;?Activity?Assays.
- 下載 英文說明書 中文說明書
- 規(guī)格 10μg50μg 200μg 1mg 5mg
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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SDS-PAGE圖
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Figure. Western Blot; Sample: Recombinant HIF1a, Human.
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通過ISO 9001、ISO 13485質(zhì)量體系認證
活性實驗
HIF1a (Hypoxia-inducible factor 1-alpha) is a subunit of a transcription factor, which functions as a master transcriptional regulator of the adaptive response to hypoxia. The protein chaperone heat shock protein 90 (Hsp90) is a major regulator of different transcription factors, including HIF1a, thus a binding ELISA assay was conducted to detect the interaction of HIF1a and HSP90. Briefly, recombinant human HIF1a were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to HSP90-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-HIF1a pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of of HIF1a and HSP90 was shown in Figure 1, and this effect was in a dose dependent manner.
用法
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
儲存
避免反復凍融。2-8°C不超過一個月，-80°C不超過12個月。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當?shù)臈l件下存儲，損失率低于5%。
增值服務
相關產(chǎn)品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應用(僅供研究使用，不用于臨床診斷！) |
| APA798Hu02 | 低氧誘導因子1α(HIF1a)活性蛋白 | Cell?culture;?Activity?Assays. |
| RPA798Hu01 | 低氧誘導因子1α(HIF1a)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA798Hu02 | 低氧誘導因子1α(HIF1a)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA798Hu01 | 低氧誘導因子1α(HIF1a)多克隆抗體 | WB; IHC; ICC; IP. |
| PAA798Hu02 | 低氧誘導因子1α(HIF1a)多克隆抗體 | WB; IHC; ICC; IP. |
| LAA798Hu81 | 低氧誘導因子1α(HIF1a)多克隆抗體(異硫氰酸熒光素標記) | WB; IHC; ICC; IF. |
| LAA798Hu71 | 低氧誘導因子1α(HIF1a)多克隆抗體(生物素標記) | WB; IHC; ICC. |
| MAA798Hu21 | 低氧誘導因子1α(HIF1a)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA798Hu23 | 低氧誘導因子1α(HIF1a)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA798Hu24 | 低氧誘導因子1α(HIF1a)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA798Hu25 | 低氧誘導因子1α(HIF1a)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA798Hu22 | 低氧誘導因子1α(HIF1a)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA798Hu27 | 低氧誘導因子1α(HIF1a)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA798Hu29 | 低氧誘導因子1α(HIF1a)單克隆抗體 | WB; IHC; ICC; IP. |
| SEA798Hu | 低氧誘導因子1α(HIF1a)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| MEA798Hu | 低氧誘導因子1α(HIF1a)檢測試劑盒(酶聯(lián)免疫吸附試驗法,小樣本) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| HEA798Hu | 低氧誘導因子1α(HIF1a)檢測試劑盒(酶聯(lián)免疫吸附試驗法,高敏型) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SCA798Hu | 低氧誘導因子1α(HIF1a)檢測試劑盒(化學發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| LMA798Hu | 低氧誘導因子1α(HIF1a)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| KSA798Hu01 | 低氧誘導因子1α(HIF1a)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻
| 雜志 | 參考文獻 |
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| Translational?Oncology | OKN-007 Increases temozolomide (TMZ) Sensitivity and Suppresses TMZ-Resistant Glioblastoma (GBM) Tumor Growth [Pubmed: 30468988] |
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| Bulletin of Experimental Biology and Medicine | Age-Specific Features of Hypoxia Tolerance and Intensity of Lipopolysaccharide-Induced Systemic Inflammatory Response in Wistar Rats [Pubmed: 30903510] |
| The role of amniotic fluid exosome, hypoxia inducible fator-1α, and inflammatory cytokine in cervical insufficiency [] | |
| scandinavian journal of immunology | Vitexin alleviates interleukin‐1β induced inflammatory responses in chondrocytes from osteoarthritis patients: involvement of HIF‐1α pathway [Pubmed: 31055848] |
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| ПОТЕНЦИРОВАНИЕ АНТИГИПОКСАНТАМИ ЭФФЕКТА ГИПОКСИЧЕСКОГО ПРЕКОНДИЦИОНИРОВАНИЯ [] | |
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| Гипоксия-зависимый транскрипционный контроль активности деструктивных изменений пародонта воспалительного и злокачественного генеза [] | |
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| Molecular Biology Reports | Umbilical cord-derived mesenchymal stem cell conditioned medium reverses neuronal oxidative injury by inhibition of TRPM2 activation and the JNK signaling?… [Pubmed:35585377] |
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