B-細(xì)胞κ-輕肽基因增強(qiáng)子抑制因子激酶β(IkBKb)活性蛋白
Active Inhibitor Of Kappa-Light Polypeptide Gene Enhancer In B-Cells Kinase Beta (IkBKb)
IKK2; NFKBIKB; IKK-beta; IKKB; I-kappa-B kinase 2; Nuclear factor NF-kappa-B inhibitor kinase beta
- 編號APJ822Hu01
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 緩沖液成份PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
- 性狀凍干粉
- 純度> 90%
- 等電點(diǎn)5.4
- 應(yīng)用Cell?culture;?Activity?Assays.
- 下載 英文說明書 中文說明書
- 規(guī)格 10μg50μg 200μg 1mg 5mg
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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Figure. SDS-PAGE
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通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
活性實(shí)驗(yàn)
Inhibitor Of Kappa-Light Polypeptide Gene Enhancer In B-Cells Kinase Beta (IkBKb), a subunit of IKK, plays a crucial role in intracellular signaling, especially in inflammation and immune responses. In addition, the binding of TRAF6 to IkBKB is an important link in the immune response, which is not only involved in the initiation of inflammatory signals, but also in its regulation, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human IkBKb and recombinant mouse TRAF6. Briefly, IkBKb was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to TRAF6-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-IkBKb pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37℃, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 μL stop solution to the wells and read at 450/630 nm immediately. The binding activity of recombinant human IkBKb and recombinant mouse TRAF6 was shown in Figure 1, the EC50?for this effect is 0.11 ug/mL.
用法
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
儲存
避免反復(fù)凍融。2-8°C不超過一個(gè)月，-80°C不超過12個(gè)月。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定，具體方法如下：在37°C孵育48小時(shí)，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲，損失率低于5%。
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| RPJ822Hu01 | B-細(xì)胞κ-輕肽基因增強(qiáng)子抑制因子激酶β(IkBKb)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APJ822Hu01 | B-細(xì)胞κ-輕肽基因增強(qiáng)子抑制因子激酶β(IkBKb)活性蛋白 | Cell?culture;?Activity?Assays. |
| PAJ822Hu01 | B-細(xì)胞κ-輕肽基因增強(qiáng)子抑制因子激酶β(IkBKb)多克隆抗體 | WB; IHC; ICC; IP. |
| MAJ822Hu21 | B-細(xì)胞κ-輕肽基因增強(qiáng)子抑制因子激酶β(IkBKb)單克隆抗體 | WB; IHC; ICC; IP. |
| SEJ822Hu | B-細(xì)胞κ-輕肽基因增強(qiáng)子抑制因子激酶β(IkBKb)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMJ822Hu | B-細(xì)胞κ-輕肽基因增強(qiáng)子抑制因子激酶β(IkBKb)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
