欧美综合自拍亚洲综合网_欧美黑人巨大videos异族_可乐操中文字幕_亚洲成av人福利专区网站_国产强奸视频在播放_免费无码国产在线53_最近最新的免費中文字幕_抖音免费视频素材_亚洲精品熟女一二区_国产无套粉嫩白浆在线麻豆

中間α-球蛋白抑制因子H4(ITIH4)活性蛋白

Active Inter Alpha-Globulin Inhibitor H4 (ITIH4)

IHRP; PK120; H4P; ITIHL1; Plasma Kallikrein-Sensitive Glycoprotein; Plasma kallikrein sensitive glycoprotein 120; Inter-alpha-trypsin inhibitor family heavy chain-related protein

  • 中間α-球蛋白抑制因子H4(ITIH4)活性蛋白 產(chǎn)品包裝(模擬)
  • 中間α-球蛋白抑制因子H4(ITIH4)活性蛋白 產(chǎn)品包裝(模擬)
  • APH776Hu03.png Figure. SDS-PAGE
  • 中間α-球蛋白抑制因子H4(ITIH4)活性蛋白 Figure. Western Blot
  • Certificate 通過ISO 9001、ISO 13485質量體系認證

活性實驗

Figure. The binding activity of ITIH4 with Grb2.
Inter Alpha-Globulin Inhibitor H4 (ITIH4) is secreted into the blood, where it is cleaved by plasma kallikrein into two smaller forms. ITIH4 has been detected only in liver, and it seems to be upregulated during surgical trauma. It may also play a role in liver development or regeneration. Besides, Growth Factor Receptor Bound Protein 2 (Grb2) has been identified as an interactor of ITIH4, thus a binding ELISA assay was conducted to detect the interaction of recombinant human ITIH4 and recombinant human Grb2. Briefly, ITIH4 were diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to
Grb2-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-ITIH4 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of ITIH4 and Grb2 was shown in Figure 1, and this effect was in a dose dependent manner.

用法

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

儲存

避免反復凍融。2-8°C不超過一個月，-80°C不超過12個月。

穩(wěn)定性

熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產(chǎn)生。保質期內，在適當?shù)臈l件下存儲，損失率低于5%。

相關產(chǎn)品

編號 適用物種：Homo sapiens (Human，人) 應用(僅供研究使用，不用于臨床診斷！)
APH776Hu01 中間α-球蛋白抑制因子H4(ITIH4)活性蛋白 Cell?culture;?Activity?Assays.
APH776Hu03 中間α-球蛋白抑制因子H4(ITIH4)活性蛋白 Cell?culture;?Activity?Assays.
RPH776Hu02 中間α-球蛋白抑制因子H4(ITIH4)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPH776Hu03 中間α-球蛋白抑制因子H4(ITIH4)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPH776Hu01 中間α-球蛋白抑制因子H4(ITIH4)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAH776Hu03 中間α-球蛋白抑制因子H4(ITIH4)多克隆抗體 WB; IHC; ICC; IP.
PAH776Hu02 中間α-球蛋白抑制因子H4(ITIH4)多克隆抗體 WB; IHC; ICC; IP.
PAH776Hu01 中間α-球蛋白抑制因子H4(ITIH4)多克隆抗體 WB; IHC; ICC; IP.
LAH776Hu71 中間α-球蛋白抑制因子H4(ITIH4)多克隆抗體(生物素標記) WB; IHC; ICC.
MAH776Hu21 中間α-球蛋白抑制因子H4(ITIH4)單克隆抗體 WB; IHC; ICC; IP.
SEH776Hu 中間α-球蛋白抑制因子H4(ITIH4)檢測試劑盒(酶聯(lián)免疫吸附試驗法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMH776Hu 中間α-球蛋白抑制因子H4(ITIH4)等多因子檢測試劑盒(流式熒光發(fā)光法) FLIA Kit for Antigen Detection.
KSH776Hu01 中間α-球蛋白抑制因子H4(ITIH4)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) Main materials for "Do It (ELISA Kit) Yourself".

參考文獻

雜志 參考文獻
Toxicology Letters Biomarkers for methotrexate-induced liver injury: urinary protein profiling of psoriasis patients. [Pubmed: 23830989]
International Journal of Medical Microbiology Proteomic demonstration of the recurrent presence of inter-alpha-inhibitor H4 heavy-chain during aspergillosis induced in an animal model [Pubmed:24360996]
Cancer Research and Treatment Inter-alpha Inhibitor H4 as a Potential Biomarker Predicting the Treatment Outcomes in Patients with Hepatocellular Carcinoma [pubmed:28724284]
Diagnostics Systemic Alterations of Immune Response-Related Proteins during Glaucoma Development in the Murine Model DBA/2J [Pubmed: 32585848]
Liver Cancer Biomarkers for locally advanced hepatocellular carcinoma patients treated with liver-directed combined radiotherapy []
留言咨詢