白介素1家族成員9(IL1F9)活性蛋白
Active Interleukin 1 Family, Member 9 (IL1F9)
IL-1F9; IL36g; IL-1H1; IL-1RP2; IL1E; IL1H1; IL1RP2; Interleukin 36, Gamma; Interleukin-1 Homolog 1; Interleukin-1 epsilon; Interleukin 1-Related Protein 2
- 編號APL621Hu01
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 緩沖液成份20mM Tris, 150mM NaCl緩沖液（pH8.0, 含有1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose和Proclin300）
- 性狀凍干粉
- 純度> 90%
- 等電點6.3
- 應用Cell?culture;?Activity?Assays.
- 下載 英文說明書 中文說明書
- 規(guī)格 10μg50μg 200μg 1mg 5mg
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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Gene sequencing
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SDS-PAGE圖
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Figure. Western Blot; Sample: Recombinant IL1F9, Human.
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通過ISO 9001、ISO 13485質量體系認證
活性實驗
IL36G (Interleukin 36 gamma), also known as IL-1F9 and IL-1H1 is a member of the IL-1 family.?The receptor for IL36 gamma is reported to be a combination of IL-1 R6/IL-1 R rp2 and IL-1 R3/IL-1 R AcP. Thus we have conducted a binding ELISA assay to detect the interaction of recombinant human IL36G with recombinant human IL1R1. Briefly, IL36G were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to IL1R1-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-IL1R1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of IL36G with IL1R1 was shown in Figure 1 and this effect was in a dose dependent manner.
用法
Reconstitute in 20mM Tris, 150mM NaCl (PH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
儲存
避免反復凍融。2-8°C不超過一個月，-80°C不超過12個月。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產(chǎn)生。保質期內，在適當?shù)臈l件下存儲，損失率低于5%。
增值服務
相關產(chǎn)品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應用(僅供研究使用，不用于臨床診斷！) |
| APL621Hu01 | 白介素1家族成員9(IL1F9)活性蛋白 | Cell?culture;?Activity?Assays. |
| RPL621Hu01 | 白介素1家族成員9(IL1F9)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAL621Hu01 | 白介素1家族成員9(IL1F9)多克隆抗體 | WB; IHC; ICC; IP. |
| LAL621Hu71 | 白介素1家族成員9(IL1F9)多克隆抗體(生物素標記) | WB; IHC; ICC. |
| MAL621Hu22 | 白介素1家族成員9(IL1F9)單克隆抗體 | WB; IHC; ICC; IP. |
| MAL621Hu21 | 白介素1家族成員9(IL1F9)單克隆抗體 | WB; IHC; ICC; IP. |
| SEL621Hu | 白介素1家族成員9(IL1F9)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SCL621Hu | 白介素1家族成員9(IL1F9)檢測試劑盒(化學發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| LML621Hu | 白介素1家族成員9(IL1F9)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻
| 雜志 | 參考文獻 |
| PLOS ONE | Interleukin (IL)-1β Is a Strong Inducer of IL-36γ Expression in Human Colonic Myofibroblasts [PubMed: 26562662] |
| Journal of Investigative Dermatology | Increased interleukin‐36γ expression in skin and sera of patients with atopic dermatitis and mycosis fungoides/Sézary syndrome [Pubmed:29314201] |
| Journal of Investigative Dermatology | For Review Only [] |
| international immunopharmacology | Acitretin inhibits IL-17A-induced IL-36 expression in keratinocytes by down-regulating IκBζ [Pubmed: 31863918] |
| J Agric Food Chem | Inhibition of HMGB1/TLR4 Signaling Pathway by Digitoflavone: A Potential Therapeutic Role in Alcohol-Associated Liver Disease [Pubmed:35212223] |
| Br J Pharmacol | Modulation of IL‐36‐based inflammatory feedback loop through hepatocytes‐derived IL‐36R‐P2X7R axis improves steatosis in alcoholic steatohepatitis [Pubmed:35481896] |
